Part Collection | Heidelberg - iGEM 2022

Part Collection

Basic Parts

partnumber namedescriptionlength [bp]part
BBa_K4344000Proteins coding sequencesamp Rtranscriptional regulator for β‐lactamase gene861basic
BBa_K4344001Promoteramp R promoterpromotor for ampicilin resistance105basic
BBa_K4344002Promotercmv enhancerconstitutive expression promoter for use in mammalian cells380basic
BBa_K4344003Promotercmv promoter 1constitutive expression promoter for use in mammalian cells204basic
BBa_K4344004PromoterCMV promoter 2constitutive expression promoter for use in mammalian cells125basic
BBa_K4344005PrimersCMV-fwd.primer for CMV promotor sequence21basic
BBa_K4344006DNAColE1high-copy-number plasmid589basic
BBa_K4344007PrimersEcoRI-HSV UL19forward primer for HSV UL19 (Part: K4344020) with EcoRI restriction site32basic
BBa_K4344008Proteins coding sequenceseGFPgreen fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)717basic
BBa_K4344009PrimersEGFP-fwd-1forward primer eGFP (Part: K4344008)20basic
BBa_K4344010PrimersEGFP-fwd-2forward primer eGFP (Part: K4344008)20basic
BBa_K4344011PrimersEGFP-fwd-3forward primer eGFP (Part: K4344008)20basic
BBa_K4344012PrimersEGFP-rev-1reverse primer eGFP (Part: K4344008)20basic
BBa_K4344013PrimersEGFP-rev-2reverse primer eGFP (Part: K4344008)19basic
BBa_K4344014PrimersEGFP-rev-3reverse primer eGFP (Part: K4344008)20basic
BBa_K4344015DNAF1 oriphage-derived origin of replication456basic
BBa_K4344016PrimersGAPDH-fwd.forward primer GAPDH (Part:22basic
BBa_K4344017PrimersGAPDH-rev.reverse primer GAPDH (Part:22basic
BBa_K4344018PrimersHindIII-HSV UL19-rev.reverse primer HSV UL19 (Part: K4344020) with HindIII restriction site35basic
BBa_K4344019Translational unitsHSV TK polyAthymidine kinase of Herpes Simplex Virus 48basic
BBa_K4344020Proteins coding sequencesHSV-UL19 AA 816:1148-6xHisUL19 gene, coding for the capsid protein (VP5) of herpes simplex virus with additional His-tag for purification1020basic
BBa_K4344021PrimersHSV-UL19-fwd-2forward primer HSV UL19 (Part: K4344020)17basic
BBa_K4344022PrimersHSV-UL19-fwd-3forward primer HSV UL19 (Part: K4344020)20basic
BBa_K4344023PrimersHSV-UL19-fwd-1forward primer HSV UL19 (Part: K4344020)20basic
BBa_K4344024PrimersHSV-UL19-rev-2reverse primer HSV UL19 (Part: K4344020)20basic
BBa_K4344025PrimersHSV-UL19-rev-3reverse primer HSV UL19 (Part: K4344020)20basic
BBa_K4344026PrimersHSV-UL19-rev.-1reverse primer HSV UL19 (Part: K4344020)20basic
BBa_K4344027Proteins coding sequencesKan Rgene for kanamycin resistance795basic
BBa_K4344028Ribosome Binding Site / aboutKozak-Seqprotein translation initiation site11basic
BBa_K4344029Promoterlac operatorlac operator17basic
BBa_K4344030Promoterlac promoterlac promoter31basic
BBa_K4344031Proteins coding sequencesNeo Rgene for neomycin resistance795basic
BBa_K4344032PrimersNotI-eGFP-fwd.forward primer eGFP (Part: K4344008) with NotI restriction site30basic
BBa_K4344033PrimersNotI-HSV-UL19-6xHis-fwd.forward primer HSV UL19 (Part: K4344020) with NotI restriction site and with additional His-tag for purification32basic
BBa_K4344034PrimersNotI-HSV-UL39-6xHis-fwd. forward primer HSV UL39 with NotI restriction site and with additional His-tag for purification35basic
BBa_K4344035Primersp19-BamHI-rev.reverse primer p19 with BahHI restriction site22basic
BBa_K4344036Primersp19-ClaI-fwd.forward primer p19 with BahHI restriction site24basic
BBa_K4344037Primersp19-HindIII-rev.reverse primer p19 with HindIII restriction site20basic
BBa_K4344038Primersp19-Seq.-fwd.forward primer for sequencing of p1921basic
BBa_K4344039Primersp19-Tac Extension-fwd.forward primer for tac-polymerase extension60basic
BBa_K4344040Plasmid backbonespEGFP-C1cloning vector4731basic
BBa_K4344041PlasmidspEX-A258-HSV-UL19-6xHiswith additional His-tag for purification3480basic
BBa_K4344042PrimerspGEX-SV40A-rev. Primer for pGEX20basic
BBa_K4344043Plasmid backbonespRK5 bbpRK5 backbone which is used in mammalia cells4661basic
BBa_K4344044PlasmidspRK5-HSV UL19 6xHiswith additional His-tag for purification5658basic
BBa_K4344045PrimerspRK5-Kozak-fwd.Primer for extinction PCR60basic
BBa_K4344046Plasmid backbonespUC19 backbonecloning vector, prefix suffix problem1926basic
BBa_K4344047PrimerspUC19-AmpR-rev.reverse primer for ampR20basic
BBa_K4344048PrimerspUC19-BamHI-fwd.forward primer for BamHI30basic
BBa_K4344049PrimerspUC19-ClaI-rev.reverse primer for ClaI35basic
BBa_K4344050PlasmidspUC19-p19-6xHis-siRNA empty (Tac-LacO-SD)with additional His-tag for purification2852basic
BBa_K4344051PlasmidspUC19-p19-6xHis-siRNA UL19 (Tac-LacO-SD)with additional His-tag for purification3346basic
BBa_K4344052PrimerspUC19-pBR322-ori-fwd.forward primer for puc19-pBR32220basic
BBa_K4344053TerminatorsRRNBT1 Terminatorterminator from e.coli87basic
BBa_K4344054PrimersSacI-eGFP-fwd.forward primer for eGFP28basic
BBa_K4344055PrimersSacI-HSV-UL19-6xHis-fwd.forward primer HSV UL19 (Part: K4344020) with SacI restriction site and with additional His-tag for purification30basic
BBa_K4344056PrimersSacI-HSV-UL39-6xHis-fwd.forward primer HSV UL19 (Part: K4344020) with NotI restriction site and with additional His-tag for purification33basic
BBa_K4344057PrimersSalI-eGFP-rev.reverse primer eGFP (Part: K4344008) with SalI restriction site28basic
BBa_K4344058PrimersSalI-HSV UL19-rev.reverse primer HSV UL19 (Part: K4344020) with SalI restriction site35basic
BBa_K4344059PrimersSalI-HSV-UL19-6xHis-rev.reverse primer HSV UL19 (Part: K4344020) with SalI restriction site and with additional His-tag for purification31basic
BBa_K4344060PrimersSalI-HSV-UL39-6xHis-rev.reverse primer HSV UL39 (Part: ) with SalI restriction site and with additional His-tag for purification29basic
BBa_K4344061Ribosome Binding SiteShine-Dalgarnoribosomal binding site in prokaryotes7basic
BBa_K4344062RNAsiRNA-eGFP-pos. cntrlsiRNA to check the knockdown-efficiency of eGFP24basic
BBa_K4344063RNAsiRNA-HSV UL19-1siRNA for silencing the UL19 HSV capsid protein25;27basic
BBa_K4344064DNAsiRNA-Linkermodified linker of the complementary RNA-sequences which leads to a loop32basic
BBa_K4344065TerminatorsSV40 Poly TerminatorSV40 Terminator122basic
BBa_K4344066PromoterT7 PromoterT7 Promoter18basic
BBa_K4344067TerminatorsT7 TerminatorT7 Terminator48basic
BBa_K4344068PromoterTac PromoterTac Promoter29basic
BBa_K4344069Primerstac-LacO-SD-p19-fwd.forward primer for tacolaco60basic
BBa_K4344070Primerstac-LacO-SD-p19-rev.reverse primer for tacolaco60basic
BBa_K4344071Primerstac-Tac Extension-rev.reverse primer for extension PCR60basic
BBa_K4344072Proteins coding sequencesTomato-Stunt p19 Protein 6xHiswith additional His-tag for purification537basic
BBa_K4344073DNAUL19-siRNA TargetUL19-siRNA Target249basic
BBa_K4344074DNAUL19-siRNA Target complementUL19-siRNA Target249basic
BBa_K4344075PrimersXhoI-HSV-UL19-6xHis-rev.reverse primer HSV UL19 (Part: K4344020) with Xho restriction site and with additional His-tag for purification31basic
BBa_K4344076PrimersXhoI-HSV-UL39-6xHis-rev.reverse primer HSV UL19 (Part: K4344020) with Xho restriction site and with additional His-tag for purification29basic
BBa_K4344077RNAsiRNA-HSV UL19-2siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV25;27basic
BBa_K4344078RNAsiRNA-HSV UL19-3siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV25;27basic
BBa_K4344079RNAsiRNA-HSV UL19-4siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV25;27basic
BBa_K4344080DNAp19-siRNA emptycasette with a p19 His-taged protein and restriction sites to insert a variations of RNA473basic
BBa_K4344081PromoterSV40 Promotorviral promoter derived from Simian Virus 40358basic
BBa_K4344082PromoterTacolacOimproved part to enhance ribosomal binding71basic

Composite parts

partnumberbasic part compositionnamedescriptionlength [bp]part
BBa_K4344083BBa_K4344082 BBa_K4344072enhanced P19-Protein translation casette(TacoLaco)-(Tomato-Stunt-p19-Protein-6xHis)-(RRNBT1-Terminator)608composite
BBa_K4344084BBa_K4344002 - BBa_K4344003 - BBa_K4344004enhanced CMV Promoter(CMVenhancer)-(CMV1)-(CMV2)709composite
BBa_K4344085BBa_K4344028 - BBa_K4344020 - BBa_K4344065Shortened Transgen Sequence UL19(Kozak)-(HSV-UL19-AA816:1148)-(SV40polyA)1153composite
BBa_K4344091BBa_K4344083 - BBa_K4344053 - BBa_K4344066 SacI-restrictionsite XhoI-restrictionsite BBa_K4344064 SalI-restrictionsite NotI-restrictionsite BBa_K4344067 p19 pulldown gene and siRNA target expression casette empty(enhanced P19)-(rrnBT1 Terminator)-(T7-Promoter)-(SacI)-(XhoI)-(siRNA-Linker)-(SalI)-(NotI)-(T7-Terminator)862composite
BBa_K4344086BBa_K4344084 - BBa_K4344085CMV regulated shortened transgen sequence(CMVenhancer)-(CMV1)-(CMV2)-(Kozak)-(HSV-UL19-AA816:1148)-(SV40polyA)1870device
BBa_K4344089BBa_K4344066 Sac XHO BBa_K4344064 Sal Not BBa_K4344067 dsRNA expression casette empty(T7 Promoter)-(SacI)-(XhoI)-(siRNA-Linker)-(SalI)-(NotI)-(T7 Terminator)132device
BBa_K4344090BBa_K4344083 - BBa_K4344053 - BBa_K4344066 - BBa_K4344073 - BBa_K4344064 - BBa_K4344073 - BBa_K4344067p19 pulldown gene and UL19 siRNA target expression casette(enhanced P19)-(rrnB T1 Terminator)-(T7-Promoter)-(UL19-siRNA-Target)-(siRNA-Linker)-(UL19-siRNA-Target-complement)-(T7-Terminator)1358device