BBa_K4344000 | Proteins coding sequences | amp R | transcriptional regulator for β‐lactamase gene | 861 | basic |
BBa_K4344001 | Promoter | amp R promoter | promotor for ampicilin resistance | 105 | basic |
BBa_K4344002 | Promoter | cmv enhancer | constitutive expression promoter for use in mammalian cells | 380 | basic |
BBa_K4344003 | Promoter | cmv promoter 1 | constitutive expression promoter for use in mammalian cells | 204 | basic |
BBa_K4344004 | Promoter | CMV promoter 2 | constitutive expression promoter for use in mammalian cells | 125 | basic |
BBa_K4344005 | Primers | CMV-fwd. | primer for CMV promotor sequence | 21 | basic |
BBa_K4344006 | DNA | ColE1 | high-copy-number plasmid | 589 | basic |
BBa_K4344007 | Primers | EcoRI-HSV UL19 | forward primer for HSV UL19 (Part: K4344020) with EcoRI restriction site | 32 | basic |
BBa_K4344008 | Proteins coding sequences | eGFP | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212) | 717 | basic |
BBa_K4344009 | Primers | EGFP-fwd-1 | forward primer eGFP (Part: K4344008) | 20 | basic |
BBa_K4344010 | Primers | EGFP-fwd-2 | forward primer eGFP (Part: K4344008) | 20 | basic |
BBa_K4344011 | Primers | EGFP-fwd-3 | forward primer eGFP (Part: K4344008) | 20 | basic |
BBa_K4344012 | Primers | EGFP-rev-1 | reverse primer eGFP (Part: K4344008) | 20 | basic |
BBa_K4344013 | Primers | EGFP-rev-2 | reverse primer eGFP (Part: K4344008) | 19 | basic |
BBa_K4344014 | Primers | EGFP-rev-3 | reverse primer eGFP (Part: K4344008) | 20 | basic |
BBa_K4344015 | DNA | F1 ori | phage-derived origin of replication | 456 | basic |
BBa_K4344016 | Primers | GAPDH-fwd. | forward primer GAPDH (Part: | 22 | basic |
BBa_K4344017 | Primers | GAPDH-rev. | reverse primer GAPDH (Part: | 22 | basic |
BBa_K4344018 | Primers | HindIII-HSV UL19-rev. | reverse primer HSV UL19 (Part: K4344020) with HindIII restriction site | 35 | basic |
BBa_K4344019 | Translational units | HSV TK polyA | thymidine kinase of Herpes Simplex Virus | 48 | basic |
BBa_K4344020 | Proteins coding sequences | HSV-UL19 AA 816:1148-6xHis | UL19 gene, coding for the capsid protein (VP5) of herpes simplex virus with additional His-tag for purification | 1020 | basic |
BBa_K4344021 | Primers | HSV-UL19-fwd-2 | forward primer HSV UL19 (Part: K4344020) | 17 | basic |
BBa_K4344022 | Primers | HSV-UL19-fwd-3 | forward primer HSV UL19 (Part: K4344020) | 20 | basic |
BBa_K4344023 | Primers | HSV-UL19-fwd-1 | forward primer HSV UL19 (Part: K4344020) | 20 | basic |
BBa_K4344024 | Primers | HSV-UL19-rev-2 | reverse primer HSV UL19 (Part: K4344020) | 20 | basic |
BBa_K4344025 | Primers | HSV-UL19-rev-3 | reverse primer HSV UL19 (Part: K4344020) | 20 | basic |
BBa_K4344026 | Primers | HSV-UL19-rev.-1 | reverse primer HSV UL19 (Part: K4344020) | 20 | basic |
BBa_K4344027 | Proteins coding sequences | Kan R | gene for kanamycin resistance | 795 | basic |
BBa_K4344028 | Ribosome Binding Site / about | Kozak-Seq | protein translation initiation site | 11 | basic |
BBa_K4344029 | Promoter | lac operator | lac operator | 17 | basic |
BBa_K4344030 | Promoter | lac promoter | lac promoter | 31 | basic |
BBa_K4344031 | Proteins coding sequences | Neo R | gene for neomycin resistance | 795 | basic |
BBa_K4344032 | Primers | NotI-eGFP-fwd. | forward primer eGFP (Part: K4344008) with NotI restriction site | 30 | basic |
BBa_K4344033 | Primers | NotI-HSV-UL19-6xHis-fwd. | forward primer HSV UL19 (Part: K4344020) with NotI restriction site and with additional His-tag for purification | 32 | basic |
BBa_K4344034 | Primers | NotI-HSV-UL39-6xHis-fwd. | forward primer HSV UL39 with NotI restriction site and with additional His-tag for purification | 35 | basic |
BBa_K4344035 | Primers | p19-BamHI-rev. | reverse primer p19 with BahHI restriction site | 22 | basic |
BBa_K4344036 | Primers | p19-ClaI-fwd. | forward primer p19 with BahHI restriction site | 24 | basic |
BBa_K4344037 | Primers | p19-HindIII-rev. | reverse primer p19 with HindIII restriction site | 20 | basic |
BBa_K4344038 | Primers | p19-Seq.-fwd. | forward primer for sequencing of p19 | 21 | basic |
BBa_K4344039 | Primers | p19-Tac Extension-fwd. | forward primer for tac-polymerase extension | 60 | basic |
BBa_K4344040 | Plasmid backbones | pEGFP-C1 | cloning vector | 4731 | basic |
BBa_K4344041 | Plasmids | pEX-A258-HSV-UL19-6xHis | with additional His-tag for purification | 3480 | basic |
BBa_K4344042 | Primers | pGEX-SV40A-rev. | Primer for pGEX | 20 | basic |
BBa_K4344043 | Plasmid backbones | pRK5 bb | pRK5 backbone which is used in mammalia cells | 4661 | basic |
BBa_K4344044 | Plasmids | pRK5-HSV UL19 6xHis | with additional His-tag for purification | 5658 | basic |
BBa_K4344045 | Primers | pRK5-Kozak-fwd. | Primer for extinction PCR | 60 | basic |
BBa_K4344046 | Plasmid backbones | pUC19 backbone | cloning vector, prefix suffix problem | 1926 | basic |
BBa_K4344047 | Primers | pUC19-AmpR-rev. | reverse primer for ampR | 20 | basic |
BBa_K4344048 | Primers | pUC19-BamHI-fwd. | forward primer for BamHI | 30 | basic |
BBa_K4344049 | Primers | pUC19-ClaI-rev. | reverse primer for ClaI | 35 | basic |
BBa_K4344050 | Plasmids | pUC19-p19-6xHis-siRNA empty (Tac-LacO-SD) | with additional His-tag for purification | 2852 | basic |
BBa_K4344051 | Plasmids | pUC19-p19-6xHis-siRNA UL19 (Tac-LacO-SD) | with additional His-tag for purification | 3346 | basic |
BBa_K4344052 | Primers | pUC19-pBR322-ori-fwd. | forward primer for puc19-pBR322 | 20 | basic |
BBa_K4344053 | Terminators | RRNBT1 Terminator | terminator from e.coli | 87 | basic |
BBa_K4344054 | Primers | SacI-eGFP-fwd. | forward primer for eGFP | 28 | basic |
BBa_K4344055 | Primers | SacI-HSV-UL19-6xHis-fwd. | forward primer HSV UL19 (Part: K4344020) with SacI restriction site and with additional His-tag for purification | 30 | basic |
BBa_K4344056 | Primers | SacI-HSV-UL39-6xHis-fwd. | forward primer HSV UL19 (Part: K4344020) with NotI restriction site and with additional His-tag for purification | 33 | basic |
BBa_K4344057 | Primers | SalI-eGFP-rev. | reverse primer eGFP (Part: K4344008) with SalI restriction site | 28 | basic |
BBa_K4344058 | Primers | SalI-HSV UL19-rev. | reverse primer HSV UL19 (Part: K4344020) with SalI restriction site | 35 | basic |
BBa_K4344059 | Primers | SalI-HSV-UL19-6xHis-rev. | reverse primer HSV UL19 (Part: K4344020) with SalI restriction site and with additional His-tag for purification | 31 | basic |
BBa_K4344060 | Primers | SalI-HSV-UL39-6xHis-rev. | reverse primer HSV UL39 (Part: ) with SalI restriction site and with additional His-tag for purification | 29 | basic |
BBa_K4344061 | Ribosome Binding Site | Shine-Dalgarno | ribosomal binding site in prokaryotes | 7 | basic |
BBa_K4344062 | RNA | siRNA-eGFP-pos. cntrl | siRNA to check the knockdown-efficiency of eGFP | 24 | basic |
BBa_K4344063 | RNA | siRNA-HSV UL19-1 | siRNA for silencing the UL19 HSV capsid protein | 25;27 | basic |
BBa_K4344064 | DNA | siRNA-Linker | modified linker of the complementary RNA-sequences which leads to a loop | 32 | basic |
BBa_K4344065 | Terminators | SV40 Poly Terminator | SV40 Terminator | 122 | basic |
BBa_K4344066 | Promoter | T7 Promoter | T7 Promoter | 18 | basic |
BBa_K4344067 | Terminators | T7 Terminator | T7 Terminator | 48 | basic |
BBa_K4344068 | Promoter | Tac Promoter | Tac Promoter | 29 | basic |
BBa_K4344069 | Primers | tac-LacO-SD-p19-fwd. | forward primer for tacolaco | 60 | basic |
BBa_K4344070 | Primers | tac-LacO-SD-p19-rev. | reverse primer for tacolaco | 60 | basic |
BBa_K4344071 | Primers | tac-Tac Extension-rev. | reverse primer for extension PCR | 60 | basic |
BBa_K4344072 | Proteins coding sequences | Tomato-Stunt p19 Protein 6xHis | with additional His-tag for purification | 537 | basic |
BBa_K4344073 | DNA | UL19-siRNA Target | UL19-siRNA Target | 249 | basic |
BBa_K4344074 | DNA | UL19-siRNA Target complement | UL19-siRNA Target | 249 | basic |
BBa_K4344075 | Primers | XhoI-HSV-UL19-6xHis-rev. | reverse primer HSV UL19 (Part: K4344020) with Xho restriction site and with additional His-tag for purification | 31 | basic |
BBa_K4344076 | Primers | XhoI-HSV-UL39-6xHis-rev. | reverse primer HSV UL19 (Part: K4344020) with Xho restriction site and with additional His-tag for purification | 29 | basic |
BBa_K4344077 | RNA | siRNA-HSV UL19-2 | siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV | 25;27 | basic |
BBa_K4344078 | RNA | siRNA-HSV UL19-3 | siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV | 25;27 | basic |
BBa_K4344079 | RNA | siRNA-HSV UL19-4 | siRNA sequence which interferes with a part of the viral capsid from the unique long (UL) sequence of HSV | 25;27 | basic |
BBa_K4344080 | DNA | p19-siRNA empty | casette with a p19 His-taged protein and restriction sites to insert a variations of RNA | 473 | basic |
BBa_K4344081 | Promoter | SV40 Promotor | viral promoter derived from Simian Virus 40 | 358 | basic |
BBa_K4344082 | Promoter | TacolacO | improved part to enhance ribosomal binding | 71 | basic |