Contribution

Overview

When we meet problems ,which are relevant with our project, we will need to cooperate with many people to improve our project. We had a two-way communication on the feasibility of the project with the Worldshaper-HZ, laying the foundation for our next experiment and publicity. With the help of the seniors from SCUT, we improved our modeling. Meanwhile, its our honor to invite them to our school for joint publicity, and we achieved good results. We also cooperated with authorities to make comic book for children aged 6-12 to spread them the introduction of food safety. These activities improve the degree of project completion, but also draw on the excellent experience of many outstanding teams.

1 Cohost of public propaganda event with seven other iGEM teams

In order to better promote our projects and synthetic biology, We held an online publicity activity with the theme of “Synthetic biology in Life” with IvyMaker China, Worldshaper-HZ, Worldshaper-HZBIOX, Worldshaper-Shanghai, Worldshaper-Nanjing, Worldshaper-NJBIOX. Our propaganda event was held online open to the public on July10th, 2022. This activity is mainly divided into five parts: introduction to synthetic biology, the application of synthetic biology in environmental protection, disease diagnosis, disease treatment and food safety testing. Our team is responsible for the food safety inspection part. We describe the three main goals we are trying to achieve in the process of developing products for bacterial pathogen detection: linking each strain with a specific RBPs, coupling nanomagnetic beads to the RBPs, and ensuring the storage security of samples that may contain bacteria. During this activity, we experienced the application prospect of synthetic biology in food safety detection. Through this joint publicity activity, we not only successfully publicized our project to the public, but also fortunately found Worldshaper-HZ with similar subject contents-cell-free system for subsequent cooperation and communication

Figure 1 Our poster for the propaganda



Figure 2 A picture of all members who took part in the propaganda on July 10th after the activity was over.

2 Communication with Worldshaper-HZ on cell-free system

As we found in the seven-team joint public announcement, we have the same experimental theory with Worldshaper-HZ, which is cell-free system. Therefore, our two teams communicated with each other through social media; we introduced principles of the projects respectively and communicated with each other on the feasibility of using other method to solve our own project. In the final analysis of experimental results, our team adopted fluorescent image to obtain concentration and the design about the app, Worldshaper - HZ in the final experimental results also need to adopt fluorescent image analysis to obtain the final experiment. Our team decided to share our experience with them to help with the analyzation of final results and refine their respective projects. In the process of communication, we built more friendships and shared the resonance of the tense life of high school students in iGEM competition.In the process of communication, our software has given a lot of inspiration to the students of Worldshaper-HZ, which may be used as a part of their future application. Their hardware design also gave us a lot of inspiration.

Figure 3 Screenshot of wechat exchange

3 Be mentored by 2022iGEM team SCUT-China

Our project experiment was mentored by Professor Wang from East China University of Science and Technology. With great honor, we reached a patnership with the SCUT China team. As high school students, we lack comprehensive understanding of professional knowledge, experimental technology and competition experiences. Therefore, during this season, we had frequent exchanges with SCUT - China, they provided us with a lot of academic suggestions and supports, such as guidance in modeling, joint campus publicity activities and so on.

Kick- off meeting

We successfully met offline for the first time in South China University of Technology. In the discussion meeting, we communicated with each other's projects, and SCUT gave us specific suggestions on mathematical modeling, hardware devices, experiments and other aspects. In the discussion, we focused on the experimental content and thought about how to improve the experimental design scheme of our team. In addition, through this face-to-face communication, we deepened our cooperative relationship with SCUT.

Figure 4 A presentation given during the collaboration meeting with team SCUT-iGEM

Public activity in Guangya High Schoo

We held an activity in Guangya High School on September 16th to introduce our topic to our schoolmates and teachers.

We started the preparation process for this activity since July 2022. There are 5 different sub-activities in the event including: the introduction of the topic of SCUT-iGEM, the introduction of the topic of Worldshaper-GYHS-iGEM, Q&A of Synthetic Biology, (plant rubbing) and the matching game of bacteriophage. In this event, we are mainly responsible for publicity and providing venues, gifts. In the activity, we gave a general plan and prepared an activity to understand different bacteriophages and the SCUT is responsible for plant rubbing and knowledge Q&A. All activities are welcomed by the students in Guangya and the whole process took us about 1.5 hours. Both teams played the Promotion Video at the time to show the details of every step during the experiment. Pictures about plant rubbing

Figure 5 Picture of plant rubbing activities

Mathematical modeling

Our team is relatively unfamiliar with the mathematical modeling module, so we invited the teacher to help us explain the specific mathematical modeling knowledge points such as Gauss process. Fortunately, we also successfully invited the seniors of SCUT-iGEM to help us sort out the modeling framework of our project in this course discussion. During the course explanation, students from South China University of Technology also raised questions to the teacher about the premise that "experimental data conform to Gaussian process" and expressed their views on this knowledge point. In order to improve our mathematical modeling ability, we actively communicated with teachers and members of SCUT-iGEM. Through the course communication, SCUT-iGEM put forward many constructive suggestions on the mathematical modeling of our team, and the teacher also inspired SCUT-iGEM's new ideas on mathematical modeling. We hope it will be helpful to them in the future. After the course, we also held several meetings, discussed two different methods, and finally decided to use the statistical method to do our next research.

Figure 6&7 Screenshot of Tecent conference video

4 Collaboration of comics book drawing

During the process of introducing our project on Instagram, we cooperated with igemtecchih, to make a short comics book for ages 6-12 children in elemental/middle school. After accepting the invitation, all our members began brainstorming about how to convey our project more readable to children with no biological knowledge reserve. Finally, after consulting with the teacher, we first briefly introduced the conceptual problem of bacteriophage in the comic book, and then roughly described the whole experiment process with stick figures to attract children’ interests and hopefully we helped them developing a strong interest in biology. In addition, it helps us to share data with other IGEM teams, facilitating our understanding of each team's topics and promoting our team's projects.

Figure 8 comics book series pictures

↑