We have established a deep partnership with several teams. We have carried out discussions on
experiments
and
modeling
and prepared
Human Practices
and
education
activities together.We were passionate about our projects though our communication was almost online due to the impact of the COVID-19.
In this process, we understood the importance of communication and mastered many effective
communication methods
. In the whole process of cooperation and communicantion, we learned from each other and achieved each other's success.
Introduction
We have maintained a close cooperative relationship with XJTLU-CHINA since the first communication on July 16, 2022. Our projects are all about
sewage treatment
. There are many aspects for us to learn from each other which both in experiments and hardware. At the same time, our team also sincerely thanks XJTLU-CHINA for their help.
The timeline about our partnership
What did we learn from each other?
We have conducted in-depth communication in
experiments
,
modeling
,
human practice
,
education
,
safety
and other aspects. We have learned a lot from each other that we have improved our own projects. And we also gained
the valuable friendship
in the process.
XJTLU-CHINA→GXU-China
1.Human Practices
XJTLU-CHINA has rich experience and has achieved great success, which has provided many guiding suggestions for our project.
On July 28, 2022, we went to Beitou Zhongmeng Water Purification Governance CO.LTD, Guangxi Nanning, China to
investigate
and
study
.
They helped to expand our
questionnaire
sample to investigate the water pollution and treatment in different areas.
We established
an alliance
which provides an exchange platform for 2022 freshwater restoration related iGEM projects and various teams jointly carry out some HP activities and education sections.
2.Experiments
We fully understand each other's projects. XJTLU-CHINA uses
biofilm
to adsorb heavy metal ions.
We received
feedback
and
opinions
from XJTLU-CHINA about experiment.
3.Modeling
They shared their team experience with us and guided us how to select the map for
residual analysis
.
We gained new ideas on fitting
the biological growth curve
with their help. And we had further communication on the feasibility of SimBiology.
The modeling team members of XJTLU-CHINA shared their experience and plans in
curve fitting
.
4.Safety
XJTLU-CHINA invited us to discuss the safety in our sewage treatment project.
GXU-China→XJTLU-CHINA
1.Human Practices
We established
an alliance
which provides an exchange platform for 2022 freshwater restoration related iGEM projects and various teams jointly carry out some HP activities and
education sections
.
We helped to expand their
questionnaire
sample to investigate the water pollution and treatment in different areas.
2.Experinent
Our project of a
blue-green light
regulated biological bacteria preparation is an engineered bacteria transformed by means of synthetic biology.
We have rich experience in the
modification
of engineered bacteria. We shared our experience and put forward some guiding opinions to them.
3.Modeling
We shared our experience and ideas in the field of SimBiology.
We believed that the continuous communication between our two teams on modeling issues will make the modeling work of the two teams more and more smooth.
4.Safety
We shared our ideas with them,like suicide mechanism of our engineered bacteria and fully enclosed hardware space.
Introduction
ZJU-China and our projects both use
Bacillus subtilis
as biological chassis. After the online meeting, ZJU-China got in touch with us and hoped to further cooperate in experiments. So we began to learn from each other and help each other in experiments in June.
What did we learn from each other?
Our communication and cooperation with ZJU-China mainly revolved around
experiments
. We learned from each other in the selection of
experimental materials
and the subsequent experimental
verification
. With their help, our project went more smoothly.
ZJU-China→GXU-China
1. We received their plasmid pHY300PLK-Pveg-GFP.
2. We successfully obtained the GFP gene we wanted from the plasmid pHY300PLK-Pveg-GFP in the later experiment.
GXU-China→ZJU-China
1. In June 2022, we sent the plasmid pHY-P43 to ZJU-China for their experiment.
2. We helped them to carry out the plasmid validation .
Water pollution is an extremely serious problem nowadays. Our iGEM teams also has many projects related to sewage treatment. We got in touch with them to exchange experience in the competition and discuss the experimental process.
We also set up
a Freshwater Bioremediation Alliance
for further exchange and cooperate. The teams participating in the alliance include Nanjing_NFLS, XJTLU-CHINA, Nanjing-China, CHINA-FAFU, GXU-China, UM_Macau and NPU-CHINA.
The aim of the alliance is to provide
a platform for communication
on iGEM 2022 freshwater restoration related projects and we can jointly develop some HP activities and educational sections.
The timeline about our alliance
The establishment of the alliance
On 27 July 2022, Nanjing_NFLS, XJTLU-CHINA, Nanjing-China, CHINA-FAFU, GXU-China and UM_Macau officially formed the Freshwater Bioremediation Alliance, which includes three main modules, science promotion, joint interviews and questionnaires.
At this meeting, we discussed the cooperation idea on the establishment of the alliance. Then, each team gave a brief introduction of their project, including experimental design, HP planning and hardware facilities, etc.
Safety
The aims of the meeting:
The main purpose of this meeting is to discuss our safety from three aspects.
1. We aim to prove that our experimental steps meet the safety standards.
2. We aim to prove that our engineering bacteria will not cause leakage.
3. We try to ensure that our projects will not cause harm to the environment and human beings.
Our proven of the safety:
1.The
Bacillus subtilis
used in the project will not cause harm to the environment and organisms.
2. We experimentally designed a light control switch, which activates the suicide mechanism when green light shines, and then kills bacteria.
3. The hardware adopts closed-loop design and the detection devices of temperature, pH, nitrogen oxide, etc. are designed, which ensures the safety to a great extent.
4. The harmful gases that may be discharged during the experimental detection process are collected and processed in a centralized manner so as to avoid being released to the environment.
Co-interview
The aims of the meeting:
We were honored to invite Professor Shen to give detailed answers to the questions raised by each team and also put forward some
practical suggestions
about our projects.
What we have gained from the co-interview?
Our team asked some questions about the experiment and modeling. Without doubt, we got a lot of
feasible advice
from Professor Shen. We also
improved our project
with the help of the useful advice.
