This page contains information about the parts created by our team.
Here is our Parts table
| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4481000 | Coding | smPAL codon optimized for E. coli | Anne Byford, Mingxiao Ma, Xining Wu | 1569 bp |
| BBa_K4481010 | Coding | scCCL codon optimized for E. coli | Anne Byford, Mingxiao Ma, Xining Wu | 1566 bp |
| BBa_K4481020 | Coding | atCCR codon optimized for E. coli | Anne Byford, Mingxiao Ma, Xining Wu | 996 bp |
K4481000 - smPAL
Part Type: Coding
Short Description: smPAL codon optimized for E. coli
Long Description: The coding sequence for phenylalanine-ammonia lyase is originally from Streptomyces maritimus. The coding sequence was codon optimized for use in E. coli using the IDT website.
Source of the part: Part was produced by IDT from a sequence published by Bang, et al (2016) paper published in Microbial Cell Factories.
Citation of the source: Bang, H. B., Lee, Y. H., Kim, S. C., Sung, C. K., & Jeong, K. J. (2016). Metabolic engineering of Escherichia coli for the production of cinnamaldehyde. Microbial Cell Factories, 15(1), 16. https://doi.org/10.1186/s12934-016-0415-9
Design considerations: The published sequence was altered to remove restriction sites without changing the ultimate amino acid sequence and then optimized for E. coli.
Link to Registry Page: http://parts.igem.org/Part:BBa_K4481000
K4481010 - scCCL
Part Type: Coding
Short Description: scCCL codon optimized for E. coli
Long Description: The coding sequence for 4-coumarate:CoA ligase is originally from Streptomyces coelicolor. The coding sequence was codon optimized for use in E. coli using the IDT website.
Source of the part: Part was produced by IDT from a sequence published by Bang, et al (2016) paper published in Microbial Cell Factories.
Citation of the source: Bang, H. B., Lee, Y. H., Kim, S. C., Sung, C. K., & Jeong, K. J. (2016). Metabolic engineering of Escherichia coli for the production of cinnamaldehyde. Microbial Cell Factories, 15(1), 16. https://doi.org/10.1186/s12934-016-0415-9
Design considerations: The published sequence was altered to remove restriction sites without changing the ultimate amino acid sequence and then optimized for E. coli.
Link to Registry Page: http://parts.igem.org/Part:BBa_K4481010
K4481020 - atCCR
Part Type: Coding
Short Description: atCCR codon optimized for E. coli
Long Description: The coding sequence for cinnamoyl-CoA reductase is originally from Arabidopsis thaliana. The coding sequence was codon optimized for use in E. coli using the IDT website.
Source of the part: Part was produced by IDT from a sequence published by Bang, et al (2016) paper published in Microbial Cell Factories.
Citation of the source: Bang, H. B., Lee, Y. H., Kim, S. C., Sung, C. K., & Jeong, K. J. (2016). Metabolic engineering of Escherichia coli for the production of cinnamaldehyde. Microbial Cell Factories, 15(1), 16. https://doi.org/10.1186/s12934-016-0415-9
Design considerations: The published sequence was altered to remove restriction sites without changing the ultimate amino acid sequence and then optimized for E. coli.
Link to Registry Page: http://parts.igem.org/Part:BBa_K4481020
The team successfully added SmPAL, ScCCL, and AtCCR into E. coli cells. All three gene sequences were codon optimized for use in E. coli. Also, we successfully added the stop codon on all the genes in the E. coli cells.