Bronze

1.All Deliverables accomplished!
2.Attributions accomplished! Please turn to Attribution for detailed information.
3.Description accomplished! Please turn to Description for detailed information.
4.Contribution accomplished! We have added new documentation to 3 existing Parts on their Part's Registry pages. Please turn to BBa_K1807009, BBa_K3022002 , BBa_K2325001 for detailed information
We have developed a rapid polyphosphate staining method and a universal way to obtain algae lysate, that is, boiling the algae. In addition to the final product phosphate, we have developed another two kinds of products: biosynthesis polyphosphate of long mixed chain-length and algae lysate. Please turn to Contribution for detailed information.

Silver

1.Engineering Success accomplished! As a manufacture project, what we concern most is the yield of the final product or the intermediate that is positively related to the final product. Therefore, this year, Engineering Cycle consisting of designing, building, testing, and learning from the data collected has been adopted in the optimization of our expression strategy. During this process, we built 3 new Parts, including BBa_K4257000, BBa_K4257011 and BBa_K4257022. Please turn to Engineering for detailed information.
2. Collaborations accomplished! To promote the progress of each other’s project, we have actively communicated and collaborated with 3 different teams, NJTech_China, NNU-China and ShanghaiTech_China, during which we have received and provided many valuable suggestions that ultimately improved each other’s project. Please turn to Collaborations for detailed information.
3. Human Practices accomplished! Throughout the whole process, we have always been devoted to promoting our project to different communities and gaining feedback and suggestions from experts from various fields. Please turn to HP-Overview for the summarization of our Human Practices. More information is displayed on the Integrated Human Practices below.
4. Proposed Implementation accomplished! We would like to share 3 sections related to our implementation: Market Prospect, Product & Application and Project Safety. Please turn to Implementation for detailed information.

Gold

1. Integrated Human Practice accomplished! Instead of treating Human Practices as the compass for only a part of actions, we decided to let Human Practices involve in every aspect of our project. So, we have constructed the Human Practices Cycle to achieve the closed loop of expression, feedback, engineering progress and building connections. For detailed information, please visit Human Practice , Education, Collaborations and Partnership .
2. Improvement of an Existing Part accomplished! We have improved 3 existing Parts. By doing so, we have obtained a new Basic Part BBa_K4257000 and a new Composite Part BBa_K4257033. Please turn to Parts for detailed information.
3. Project Modeling accomplished! We have designed our model to calculate the prediction of phosphate production and concurrent carbon fixation on terrestrial planets, assisting us to fully comprehend the possible value of our project. Please turn to Model for detailed information.
4. Proof of Concept accomplished! Our Proof of Concept verifies the concepts, theories and methods applied to our project, based on which we have developed several conceptual methods and products in addition to the final product. By subjecting these methods and products to user test, we have received user feedback, collected information about market demand, located target audience and also discovered the critical pain points. Please turn to Proof of Concept for detailed information.
5. Partnership accomplished! In view of our fruitful cooperation with NJTech_China in expressing strategies, we have further developed partnership with them throughout our 2022 iGEM project. The common goal for both teams is "providing available carbon source required for the engineered chassis in the process of making our project realistic on terrestrial planets". Please turn to Partnership for detailed information.
6. Education & Communication accomplished! We have carried out 5 types of educational activities for different audience: students in high schools and universities, grown-ups and internet users. Our materials designed for these activities are portable, accessible, and the phenomena are immediately observable. Please turn to Education and Communication for detailed information.

iGEM Special Prizes

Best Education

Education and public communication are of great importance to us. Young students in high school and colleges, the protagonists of the future development of modern technology and human society, are our primary target audience. We organized various forms of educational activities tightly connected with our research topic and developed novel educational materials for them. Meanwhile, in order to encourage communications and active participation of the target audience, our materials are portable, accessible, and the phenomena are immediately observable. Besides, in order to make iGEM and synthetic biology known to a wider audience, visiting communities and running social media is also used as the means of communication and education. Based on this, we have carried out five types of educational activities. Please turn to Education and Communication for detailed information.

1. Entering high school classrooms

Before the summer vacation, we entered Qingjiang Middle School. Through PowerPoint presentations, explanations and classroom interactions, the students were shown the crucial role of phosphorus in social production and daily lives. Furthermore, to let students truly experience the phosphorus around us, we took out the materials prepared for on-site demonstration experiments and conducted experiments on the cola purchased by the students. The rapid chromogenic reaction visually demonstrated the “daily phosphate” accessible to them every day.

The video of the experiment can be found on Education and Communication.

2. Jumping out of high school classrooms

During the summer vacation, we led some students to Purple Mountain Observatory in Nanjing, and invited specialists to give a live explanation to the students. They learned about the differences between the environment of terrestrial planets and Earth, getting a glimpse of giant astronomical telescopes and meteorites by themselves. We taught them that it is the difference in environmental conditions that leads to phosphorus on terrestrial planets being in lower oxidation state and hard to be converted into phosphate to support manufacturing activities of human society. And that is exactly the reason why we need to manufacture phosphate. We prepared elaborate tickets with team logo, iGEM logo and a picture of Purple Mountain Observatory for them as a souvenir.

3. Into the college campus

We designed a simple and rapid chromogenic reaction which is able to discriminate between phosphate and phosphite, prepared relevant materials including colorimetric cards and went into the campus of China Pharmaceutical University. Such a novel form soon attracted the attention of students. As was expected, they came up with a bunch of questions after trying by themselves, especially about phosphite which they were unfamiliar with. We passed on to them the perception of phosphorus forms on terrestrial planets, the importance of phosphate and the necessity of using phosphite as a substrate to manufacture phosphate.

The video of this outside demonstration is available on Education and Communication.

4. Deep into communications with communities

Our team members went into the community and carried out one-to-one communication with the residents there. We distributed souvenirs such as mugs and key rings printed with the theme of our education project for free after communication, so that they could take our project home. Once the youngsters at home asked them about where these stuffs came from, they would probably tell them stories about phosphorus, the outer space and synthetic biology.

5. Running social media

It is the universal mission for every iGEM team and iGEMer to make synthetic biology known to the public. Synthetic biology, a systematic discipline involving technology for engineering life systems, is very likely to give birth to the next biotechnology revolution. Considering our project's unique feature, we decided to deliver series of public lessons about synthetic biology in Bilibili, a Chinese mainstream social media site targeting specially at young people.