We have built 5 new Basic Parts and 5 Composite Parts in total. Five Composite Parts are used to express CA,CA2,PRK,CA-PRK and CA2-PRK respectively.
| Description | Type | Name |
| CYC1 | Terminator | BBa_K3812006 |
| BW25113 | RBS | BBa_K3812011 |
| 6xHis-tag | Tag | BBa_K3812012 |
| Paox1 | Promoter | BBa_K3812005 |
| pSB1C3 | Plasmid | BBa_K3812014 |
| CA2 | Coding | BBa_K4112005 |
| CA | Coding | BBa_K4112006 |
| PRK | Coding | BBa_K4112007 |
| CA2-PRK | Coding | BBa_K4112008 |
| CA-PRK | Coding | BBa_K4112009 |
| Description | Type | Name |
| CA2 | Composite | BBa_K4112010 |
| CA | Composite | BBa_K4112011 |
| PRK | Composite | BBa_K4112012 |
| CA2-PRK | Composite | BBa_K4112013 |
| CA-PRK | Composite | BBa_K4112014 |
The PTCA-encoded carbonic anhydrase of P. tricornutum was cloned by overlap PCR. As a member of the β-CA family, it can reversibly catalyze the CO2 hydration reaction, which can improve CO2 conversion efficiency and play a key role in CCM, as well as in ion exchange, CO2 acquisition, and photosynthesis.
PTCA2, which is highly homologous to PTCA, encodes a protein with fully conserved β-CA zinc coordination residues and significant levels of mRNA accumulation at low CO2 concentrations.
PRK encodes phospho-ribulose kinase, an enzyme specific to the Calvin cycle, which plays a crucial role in regulating the flow of sugars in the Calvin cycle by catalyzing the formation of the receptor ribulose-1,5-bisphosphate of CO2 with the help of ATP phosphorylation.
Use linker to connect them separately, build CA-PRK and CA2-PRK, transferred to E. coli and tested.
