Prof. Wenxiong Lin

Vice President of Fujian Agriculture and Forestry University, Professor, Doctoral Supervisor

In the past five years, he has presided over more than 20 national and provincial research projects

Published more than 200 academic papers at home and abroad

Long-term teaching and research work in rice physiology and molecular ecology, crop chemosensitization and ecological agriculture, etc.




Question

1. Our team have made hardware models, but it is difficult to widely use them due to the cost problem, how can we break this model?

2. We have planned to enter the campus lectures and academic seminars, but the format is rather limited and not interesting enough, how do we attract the public's attention?

3. We are worried that the format of campus lectures and academic talks is rather old-fashioned, how can we effectively close the distance of scientific communication with the public?

4. Do you have any comments on the management of an interdisciplinary and professional team like ours?

5. Eutrophication of water bodies often causes algae to grow like crazy, for this problem, can we turn the environmental problems into a resource to use, such as algae fertilizer, diatomaceous mud, etc., how do you think the feasibility?

6. How do you think science popularization can leave a deeper impression on people?

7. How to avoid homogenization of science popularization?

8. Should the high density culture of microalgae in wastewater be closed culture?

9. We want to spread the knowledge of biology to more rural students in remote areas, what methods can we use?

Prof. Zonghua Wang

Dean of Minjiang College

National Outstanding Young and Middle-aged Experts, Outstanding Experts of Fujian Province

Cultivated by Fujian Province inter-century academic leaders

Presided over a number of scientific research projects including the National 973, industry programs and the key projects of Fujian Provincial Natural Science Foundation

Currently engaged in research on molecular biology of plant-microbe interactions and molecular plant pathology


Question

1. Hello teacher, you just mentioned that the heavy crop problem leads to the decrease of fertility of soil pollution, I would like to ask you if you have done any research on the problem of restoring land fertility, and in this regard, is there any possibility for microalgae to help restore it?

2. Does closed culture inhibit the activity of microalgae in wastewater?

3. Do you have any suggestions for the long-term construction of our team?

4. How do we raise the visibility of our team to the public and get more like-minded people to join us?

5. Do you have any suggestions on how to put our hardware model into commercial use, given its high cost?

6. We hope to break the traditional teaching model, how to combine online and offline teaching what do you think about it?

7.The relationship between water quality and algae in aquaculture and how to effectively control them.

8. The use of a single strain of algae for sewage disposal requires disinfection or sterilization of the sewage, the stability of the system is poor, and microalgae can easily die due to contamination by foreign species, which requires high requirements for cultivation equipment and is not conducive to practical application. Do you have any solution for this?

Prof. Xinhua Chen

Xinhua Chen, Ph.D. He is a recipient of the National Outstanding Youth Fund, a leading talent in science and technology innovation from the Ministry of Organization, a leading talent in science and technology innovation among young people from the Ministry of Science and Technology, a candidate of the National Hundred Million Talents Project, and a young and middle-aged expert with outstanding contribution. He was awarded the honorary titles of National Outstanding Science and Technology Worker, Outstanding Science and Technology Worker of Fujian Province, and Leading Talent of Science and Technology Innovation of Fujian Province, etc. He enjoys the special allowance of the State Council Government.

In recent years, he has presided over 15 projects, including the key projects of the National Fund, the National Outstanding Youth Fund, the "973" program, the key projects of the Cross-Straits Joint Fund and the Special Project for Marine Public Welfare.


Question

1 Do you have any suggestions for our project?

2 Can we cultivate algae for farming effluent?

3 What are the points to note in treating farming effluent?

4What do you think are the other parts of the marine resources that can be used at present?

5. What do you think is the prospect of promoting the technology of microalgae treatment of sewage?

6 what do you think we can do to reduce the cost of microalgae in order to put them into production more quickly?

Prof. Qiang Wang

He received his Ph.D. degree from the Institute of Botany, Chinese Academy of Sciences, Beijing, China, in 2002, and conducted independent research at Hebrew University, Israel, University of Tennessee, University of Arkansas, and Michigan State University from October 2002 to July 2010. In January 2019, he was selected as a Distinguished Professor of Henan University's "Distinguished Talent Special Zone Support Program". He has led or participated in more than ten projects of the National 973 Program, National Key Research and Development Program, National Natural Science Foundation of China, Provincial Natural Science Foundation of China, and Innovation Groups, as well as frontier projects of CAS and the Institute.




Question

1.The multi-algae system has a higher removal rate of pollutants in the wastewater and is more resistant to the external environment, but we need to further explore the relationship between the algae strains in order to build an efficient multi-algae system, how to explore this relationship?

2. How to cultivate the tacit understanding and improve the relationship between the players?

3. Bacteria and algae symbiosis is a more promising application of sewage disposal wrist, compared to single algae and multi-algae disposal system, the use of bacteria and algae symbiosis disposal of sewage on the external environment is more tolerant, good biomass sedimentation, disposal costs are lower. However, the sewage disposal mechanism, community structure and the role of bacteria in the symbiosis are not understood, is there any way to improve it?

4. Compared with other types of wastewater, urban wastewater is less toxic and has a lower content of pollutants such as COD, so it is easy to use microalgae for disposal, but we have encountered difficulties in the promotion path, do you have any suggestions?

5. Although microalgae can effectively remove N, P and other nutrients in the sewage, but due to the poor biochemical properties of the methane, the simple use of microalgae on the methane COD removal is poor, what other methods can be used in combination to achieve better management results?

6. Some food industry wastewater is inhibited by high chromaticity and organic load, so when using microalgae to treat this kind of wastewater, it usually needs to be diluted or used in combination with other methods. Is there any way to break this barrier?

7. How to put the project precisely and commercialize it?

Yi Tao Associate Professor

Associate Professor, Master's Supervisor, Tsinghua University

Shenzhen Overseas High-Level Talent

Member of International Water Association (IWA)

Senior Member of Chinese Society of Environmental Sciences

Reviewer of high level SCI journals in environmental field: water research, scientific reports, earth science, etc.




Question

1. algae bacteria mutualism, what bacteria are generally selected, is it different algae, to select different bacteria? Is there any selection principle?

2. With so many advantages of algae bacteria technology in treating wastewater, how is it currently used in practice?

3. What efforts have been made in the micro and macro design of the technology regarding the biosafety in the process of application?



Jin Zhou Associate Researcher

Associate Researcher, Tsinghua University, M.S. Supervisor

Visiting Scholar, Woods Hole Oceanographic Institute, USA

Shenzhen Outstanding youth, Shenzhen Overseas High-Level Talents

Member of International Society for Microbiology and Applications

Associate Editor of SCI journal Frontier in Microbiology




Question

1. How to improve the interesting nature of science and technology while retaining its depth?

2. Are there any alternatives or shortcomings for some components of the hardware model?

3. How to motivate people when participating in a longer period of competition?

4. The difficulty of algal cell separation and harvesting is an important bottleneck limiting the large-scale industrial application of microalgae technology. The common methods of microalgae separation and harvesting include centrifugation, filtration (including membrane filtration), air flotation, direct gravity sedimentation and flocculation. Centrifugation is a fast and reliable separation and harvesting method. However, due to its very high energy consumption and investment operating costs, are there any affordable alternatives?

5. What do you think is the market prospect of microalgae technology for wastewater treatment?

6. What other activities or activities do you think we can do to show the humanism of our team?

Prof. Chao Zhao

Professor and Doctoral Supervisor, Fujian Agriculture and Forestry University

Outstanding young research talent of the university and "Jinshan Scholar".

Outstanding Young Scientist of Fujian Province, etc.

Chaired the National Natural Science Foundation of China (2 projects), special key projects in Fujian Province, and other vertical and horizontal projects

Currently engaged in research on algae chemistry and molecular nutrition




Question

1. Do you have any good harmless treatment methods for farming wastewater?

2. Do you have any suggestions for the future plans of our team?

3. In an open environment, the digestate can easily harbor bacteria, algae and their predators such as rotifers, which makes the microalgae cells vulnerable to pests during the treatment of digestate with microalgae. Therefore, pretreatment of the digestate is crucial to the entire microalgae culture process. So do you have any comments on the operation of this pre-treatment process?

4. What do you think is the value of the by-products of microalgae decontamination?

5. What is the effect and difference of microalgae wall culture on the treatment of methane wastewater?

6. How to select suitable microalgae according to different scenarios?

Prof. Yanling Zhao

He is also the president of the International Allelopathy Society, the board member of the International Society of Chemical Ecology, and the editorial board member of the International Journal of Entomology (Annual Review of Entomology, SCI journal, IF 13.02). He is also the President of International Allelopathy Society, the Director of International Society of Chemical Ecology, the Editorial Board of Annual Review of Entomology (SCI journal, IF 13.02), the Academic Editor of PLOS ONE (IF 3.53), and the Editor of International Journal of Chemical Ecology (IF 3.53). (He is a member of the editorial board of Journal of Chemical Ecology (SCI journal, IF 2.23) and the editorial board of Journal of Applied Ecology. He is in charge of the National Bilingual Teaching Demonstration Course and Guangdong Province Excellence Course.



Question

1. Do you think it is feasible to combine isolated microorganisms and sterile systems to reveal the causal relationship and interactions between root microorganisms and plant growth phenotypes?

2. Can you briefly tell us how you applied high-throughput isolation culture technology to the isolation of marine important microorganisms?

3. Do you agree with the theory that microorganisms, as a special group of organisms with extremely diverse lifestyles, play an important role in the expansion of mankind to the outer planets?

4. Do you think that the results of microbial genomic research can be transferred to development with shorter application period and higher economic value?

5. Do you think that the study of the structure and function of microbial genomes can provide innovative theoretical research in the fields of medicine, industry and agriculture?

6. Have you encountered any difficulties in the process of comparative genomics research?

Prof. Rensen Zeng

He is also the president of the International Allelopathy Society, the board member of the International Society of Chemical Ecology, and the editorial board member of the International Journal of Entomology (Annual Review of Entomology, SCI journal, IF 13.02). He is also the President of International Allelopathy Society, the Director of International Society of Chemical Ecology, the Editorial Board of Annual Review of Entomology (SCI journal, IF 13.02), the Academic Editor of PLOS ONE (IF 3.53), and the Editor of International Journal of Chemical Ecology (IF 3.53). (He is a member of the editorial board of Journal of Chemical Ecology (SCI journal, IF 2.23) and the editorial board of Journal of Applied Ecology. He is in charge of the National Bilingual Teaching Demonstration Course and Guangdong Province Excellence Course.


Question

1. Do you have any knowledge about synthetic biology in your research on the chemical ecological mechanism of insect and disease resistance and its signal transduction pathway?

2. Do you have any opinion on the mechanism and control of invasive alien organisms?

3. Do you think it is possible to translate basic scientific discoveries into applications in the field environment through the rational design and application of synthetic microbial communities with plant growth-promoting properties?

4. Is the construction of plant-associated microbial communities random or based on the basic rules of community construction?

5. Can traits such as drought tolerance and disease resistance associated with specific microbiota be inherited from the parent plant to the offspring, suggesting that the importance of host genetics and host-associated microbiota are interrelated?

6. In what ways do you think our project can be integrated with your research?

Prof. Yuan Qin

President of School of Life Sciences, Fujian Agriculture and Forestry University

High-level B talents introduced in Fujian Province

The second prize of Fujian Natural Science Award in 2020 (the first finisher)

"Recipient of National Excellent Youth Science Foundation

Recipient of the "Newton Senior Scholarship" of the Sino-British Talent Program

Fujian Province "Hundred Talents Plan", Fujian Province Leading Talents of Universities




Question

1. Hello teacher, how do we widely apply microalgae to the wastewater treatment system?

2. How do we selectively cultivate algae that can purify sewage?

3. How can we make microalgae synonymous with the team and make them widely known to the public?

4. How to make microalgae multiply quickly and efficiently?

5. What do you think about how to introduce microorganisms and microalgae into the classroom?

6. Do you have any suggestions for the long-term development of our team and how our team will improve our project?

7. It is known that the pipeline closed culture is a new culture method, which is mainly used for algae culture with high economic value and easy pollution.


Prof. Xiangying Wei

He is currently the Deputy Secretary of the Party Branch of the Marine Research Institute of Minjiang College. He is mainly engaged in the research on the positive effects of plant-microbe interactions and their mechanisms. Currently, he is mainly engaged in the research on the remediation effects of plants, microorganisms and plant-microbe interactions on environmental pollution and their mechanisms. He has presided over 4 projects of National Natural Science Foundation of China and Fujian Provincial Natural Science Foundation of China, and participated in many projects such as National Key Support Project.




Question

1. Hello, teacher, what is the scope of marine economy? How to develop the ocean economy?

2. What do you think about marine ecology, what do you think microalgae can do?

3. How can we incorporate marine ecology into our team's development goals?

4. Our hardware facilities are more expensive and require more critical technology, how can we break through this shortcoming?

5. Do you have any suggestions for team building?

6. How can we expand our team's visibility?

Communication with Professor Wenxiong Lin

Surely and Support

We encounter some difficulties at the beginning of our project. Professor Lin Wenxiong helped us a lot when we were looking for a new way out. We made a hardware model , but it was difficult to be used widely, because of the cost. Professor Lin told us that the products we made may not currently make great economic profits in the market, but as long as the products are valuable and can help achieve carbon neutrality, it is a valuable product, and in the future we can continue to carefully study ways to reduce cost. In addition, in the education section, we have planned to enter the campus to lecture and hold academic exchanges , but the forms are rather limited and not interesting enough, and we are worried that it will be difficult to attract public attention. After hearing this, Professor Lin first affirmed that our idea was scientific and he expressed his willingness to help us call on the public to pay attention to the water environment management problem. This gave us great encouragement and confidence.

Unique Advice

We were worried that the format of campus lectures and academic lectures were rather rigid and can not effectively shorten the distance between scientific communication with the public. Professor Lin gave us an easy-to-understand example: when watching the cartoons when I was a child, I still remember the characters very clearly. Why is this? Because the animation or cartoon cartoonizes the boring stories, and the interesting conversations make it easier for us to remember. Therefore, when carrying out education and publicity, more scientific knowledge can be personification, which is more conducive to the dissemination. This suggestion helped us open our minds, and we also listened to Professor Lin's advice, and made special hand-painted books to support the reading from primary school to college, and even for adults.

Project Impact

After we finished making the science book and hand-painted book, we contacted Professor Lin to get some guidance and suggestions. Professor Lin admired our science book and hand-drawn book while flipping through them and reaffirmed our approach. After reading them carefully, Professor Lin suggested some changes on the framework to help us further improve the book content, which helped us a lot.

In short, the communication with Wenxiong Lin promoted the progress of the project, and we also got the idea to solve the problem. At the same time, we are very grateful to Professor Wenxiong Lin for his support and encouragement to our project, which is of great significance to put the laboratory results into practical production and application.

Interview with Prof. Qiang Wang

Our Premise

On September 29th of this year, we conducted an interview with Prof. Qiang Wang after the Chinese Society of Plant Physiology and Molecular Biology. We benefited a lot from this activity, and through the professor's guidance, we also understood the future plan and prospect of the project development, which is very meaningful.

Serious Inquiries and Rigorous Answers

In this process, we firstly introduced the composition of our team and and the role of each classification to Prof. Qiang Wang. The professor listened carefully to the composition of our team and gave us some suggestions, such as the appropriate division of labor in the team. We also learned that Prof. Wang's research interests are mainly in algal biology, photosynthetic environmental stress response and synthetic biology. We also learned that Prof. Wang's research is focused on photosynthesis, environmental stress response and synthetic biology, and that he has taken photosynthesis research model materials, cyanobacteria and Chlorella, as the research objects, based on photosynthesis and synthetic biology, focusing on environmental problems, and conducting systematic research around scientific problems such as microalgae environmental adaptation, bioenergy and bioreduction, which is closely related to our subject direction and research direction.

Then we introduced our team's research direction to Prof. Qiang Wang, the feasibility of microalgae for wastewater treatment and the feasibility of extracting the high economic value products Phaeodactylum tricornutum,EPA and biodiesel from microalgae for wastewater treatment, Prof. as the research objects, based on photosynthesis and synthetic biology, focusing on environmental problems, and conducting systematic research around scientific problems such as microalgae environmental adaptation, bioenergy and bioreduction, which is closely related to our subject direction and research direction. Then we introduced our team's research direction to Prof. Qiang Wang, the feasibility of microalgae for wastewater treatment and the feasibility of extracting the high economic value products Phaeodactylum tricornutum,EPA and biodiesel from microalgae for wastewater treatment, Prof. Qiang Wang spoke highly of our ideas and gave full affirmation to our ideas, he explained to us that microalgae have fast growth rate, high CO2 fixation efficiency and He explained to us that microalgae have high growth rate, high efficiency of carbon dioxide fixation and photosynthesis, and can absorb and degrade the pollutants in the sewage during the growth process, thus playing a role in purifying the water. In addition, the application of microalgae in wastewater treatment has the following advantages: the use of microalgae biomass for fertilizer production, the recovery of assimilated nitrogen and phosphorus, and the resulting microalgae biomass can be used for the production of biofuels, functional foods, animal feed and pharmaceuticals, etc. In addition, microalgae reoxygenate wastewater through photosynthesis. Based on these advantages, more and more researchers are focusing on the use of microalgae to treat wastewater, leading to the rapid development of this technology and its gradual move towards practical engineering applications. We asked the professor about the advantages of microalgae treatment of sewage and feasible methods, and Professor Qiang Wang explained to us, "Microalgae assimilate and absorb pollutants such as nitrogen, phosphorus, heavy metals and organic matter in sewage during the growth process to purify the water, which greatly reduces the treatment cost and the chance of secondary pollution compared with physical-chemical methods. In future work, the focus should be on the practical application of microalgae treatment of wastewater and biomass production coupling technology to fully exploit the economic potential and environmental benefits of microalgae.

However, there are still some technical problems to be solved for the industrialization of this technology. Firstly, it is necessary to domesticate and screen high quality algae species that effectively purify water and select those with high content of oil or bioactive substances. Secondly, an economical and efficient technology for separating and harvesting microalgae should be developed. Finally, a light reactor suitable for the growth of microalgae and which can be operated on a large scale should be developed."

Wishes and Prospects

At the end of the interview, we had a group photo with Prof. Qiang Wang. The use of microalgae to treat wastewater provides an environmentally friendly path to alleviate water pollution. It is believed that with the development and breakthrough of technology, the water purification and biomass production coupling technology based on microalgae culture will have a broader prospect in the future when the shortage of resources and energy is becoming increasingly severe.

Interview with Professor Yanling Zhao

Introduction

We often encounter problems in the course of our experiments that cannot be solved by our team members' discussions, so our team keeps a wide open-minded and inquisitive attitude. Seeking help from some experts and scholars can further expand the team experiments and get more novel ideas at the same time.

Communication

Prof. Yanling Zhao is mainly engaged in marine microbial ecology research, and conducts microbial genomics and comparative genomics research with marine dominant bacteria and phages of great ecological significance.

Considering Professor Zhao's area of expertise, we also targeted some questions. Prof. Zhao pointed out that besides purifying the seawater environment and assistant marine carbon balance, we also need to pay attention to the conservation of marine diversity. Therefore, we also consider the fixation of algae strains in the design of the laboratory to prevent them from spreading in the water column and causing pollution. Prof. Yanling Zhao also gave us valuable advice on the genetic recombination of Trichoderma reesei. Prof. Zhao mentioned that we must consider all possibilities when designing experiments, which is a great inspiration for our experiments. Considering the possibility of accidental infection, we will do more biological replicates and repeat sterilization during the operation. When the results of several experiments are inconsistent, we will review the literature to see if the protocol needs to be changed. In the recombinant lab we usually design several protocols to avoid unexpected effects on the results. At the same time, she gave great recognition to our project, "These microorganisms have a huge number and diversity, and what researchers know about them now is just the tip of the iceberg, and it is very meaningful to keep discovering and exploring the value of using microalgae in research to apply them in real life."

Aid

"The burden of cultivating a new generation of young talents is not easy, and I often reflect on myself: what do students need? How to guide students well? Is the guidance adequate?" In Prof. Yanling Zhao's view, the primary responsibility as a graduate student mentor is to guide students well, provide them with a platform for research, and stimulate their creative ideas. She always hopes to spare more time to guide students and promote joint progress. When students encounter difficulties in their experiments, she will work with them to find the reasons for their failures, trace every step and process of the experiment, and find solutions to problems from analyzing the source. Prof. Zhao's words also inspired our team to be more creative and to stimulate their interest in learning when the project reaches out to people of all levels. In the subsequent "Blue Carbon" sessions, time was set aside for students to ask questions and organize craft activities to generate interest.

Vision

With the industrialization of cities and the improvement of people's living standards, more and more domestic and industrial wastewater is generated. The traditional wastewater treatment process turns the organic carbon and ammonia nitrogen in the wastewater into carbon dioxide and nitrogen gas to the atmosphere through massive aeration and alternating anaerobic and aerobic operation, without recycling in the form of energy or resources. With the depletion of fossil energy and the increasing greenhouse effect, the search for more energy-efficient and environmentally friendly wastewater treatment processes has become very urgent. We hope that Brown Finger Algae Triangle will soon be applied as an effective product for wastewater treatment at a low cost.

Interview with Professor Rensen Zeng

Communication

We introduced our project principle to president Zeng in the form of a PowerPoint presentation, which intends to use Phaeodactylum tricornutum with multiple iterations of PTCA and other genes to sequester carbon to slow down global warming. We also aim to improve the ability of microalgae to adsorb heavy metal elements to solve the problem of excessive sewage discharge. President Zeng believes that algae are a good entry point to mitigate climate change, as they do not take up arable land and are not harmful to the ecosystem, but it is worth noting how to fix and apply this tiny organism. In response to this currently unsolved problem of algae fixation, President Zeng gave us new ideas. He pointed out that the algal spheres might block the material flow and that muddy materials might be a better choice. The team members also said they would try more here.

In addition, we showed Principal Zeng the activities that the team has already held in the education section, such as environmental walk, tri-

fold distribution, etc. Principal Zeng gave us high praise and said that this is a field worth exploring, which is full of opportunities and possibilities despite the treacherous road.

Unique Advice

President Zeng pointed out that our PPT was not clear enough and that we could use the carbon and nitrogen cycle diagrams to analyze the links with high emissions to design experiments more realistically, and we could use videos or animations to explain the project process more clearly and intuitively. When reporting the results, we can also use diagrams to express the purification effect of Phaeodactylum tricornutum.







Project Impact

The affirmation from Principal Zeng gave the team confidence that our efforts would allow more audiences of all ages to learn about this algae that does not take up arable land and produces high value by-products. It also provided us with new ideas for our project presentation, learning a new way of visualizing and persuading the cycle diagram.

Interview with CEO Jinjian Xie of Fujian Shenliu Group

Introducation

Many iGEM projects can eventually be put into actual production applications, especially for us who eventually want to put the technology into practice. To move to actual production based on the theoretical basis of the laboratory, this is definitely not enough for the experiments done in the laboratory, which involves other factors such as economic interests, production efficiency, social needs and so on. And there are many processes and difficulties that we don't understand. That means we need to talk to a business that is actually going into production. There is a company in our local area, Fujian Shenliu Group, which has researched on the production and application of spirulina. On July 3, we decided to go to Fujian Shenliu Group to conduct research and interview Xie Jinjian, the person in charge of the group, in order to understand the company's understanding of pollution control technology and its opinions on microalgae treatment technology. Through this interview and communication, we have learned about the company's views on advanced sewage treatment methods and the development status of local pillar industries, have a deeper understanding of the application of project technology, and have also promoted the practical application of our technology.

Pollution Control Cost

Since Fujian Shenliu Group has researched on the production and application of spirulina, we have raised questions about the company's current technical or cost difficulties in treating sewage. We believe that at the technical level, the use of microalgae to treat wastewater is an ecologically sustainable technical method, which can absorb and degrade pollutants in wastewater by exerting the synergistic effect of algae and bacteria. The entire treatment process is not only low in energy consumption, but also promotes the recycling of nutrients such as nitrogen and phosphorus, and at the same time achieve the purpose of controlling greenhouse gas emissions. At the cost level, we believe that the techno-economics are not recognized, so it is necessary to carry out comprehensive development to improve the sustainability of the technology and reduce the cost, and we also consider that the environmental risks are also to be further evaluated. In general, large-scale production and utilization of microalgae remains a technical challenge. Accordingly, Xie Jinjian replied: Different treatment processes will produce different operating effects, and operating costs will also be different. Large-scale sewage treatment plants mostly use traditional activated sludge method and its deformation process, while small-scale sewage treatment plants mostly use oxidation ditch process. However, he believes that microalgae wastewater treatment technology is a green technology with great potential and has broad market prospects. We need to investigate new microalgae wastewater treatment processes from the aspects of pollutant treatment efficiency, process energy requirements and economics, pay attention to product development of algae, propose sustainable treatment technologies, and promote wider application of microalgae wastewater treatment. This may be related to the fact that most of the wastewater treatment technology with microalgae stays in the laboratory stage. For Shenliu Group, the annual expenditure on wastewater treatment is a large amount. Therefore, they do not know much about the cutting-edge sewage treatment technology.

School-Enterprise Cooperation

We also raised the question of where to start our research projects if we want to cooperate with companies. Because our project is currently progressing slowly in cooperation with enterprises, in this regard, we would like to have practical cooperation with Fujian Shenliu Group Company. Fortunately, Xie Jinjian believes that school-enterprise cooperation and the combination of production and education are good ways. He said frankly that he hopes to achieve resource sharing and win-win by establishing a work-study practice base. Let the advanced corporate culture enter the campus, enterprise management into the classroom, production management into the base, in this way to help improve the quality of talent training. It is reported that Fujian Shenliu Group has achieved remarkable results in talent training through several years of school-enterprise cooperation, and team members have also gained a deeper understanding of the company's culture through this exchange and practice exchange to promote the actual promotion of our project.

Project Impact

Through this communication with the company, we learned a new observation angle. From the market point of view, it is necessary to investigate the new microalgae wastewater treatment process from the aspects of pollutant treatment efficiency, process energy consumption and engineering economy. In particular, we should pay attention to the product development of microalgae, propose a sustainable development route based on the concept of circular economy, and use the microalgae treatment wastewater technology as the source rather than the terminal of the microalgae product development process, so as to promote the wider application of microalgae wastewater treatment. President Xie Jinjian's introduction to the historical development of Shenliu Group also made us understand the company's views on sewage treatment methods and the development status of local pillar industries, and gained a new understanding of the sewage treatment work carried out in this project.

Reflect and Further Improve

We are very grateful to have this opportunity to interview the president of Fujian Shenliu Group Xie Jinjian while conducting on-the-spot investigation of Fujian Shenliu Group. Throughout the year, we encourage dialogue with different groups, as we hope to measure the practical significance of our projects. Regarding the suggestion given to us by President Xie Jinjian, we have determined that if we have the opportunity to personally go to the enterprise to practice, the practical application of the project will be greatly improved.

Interview with the Head of Fuzhou Jinshan Wastewater Plant

Introduction

In a meeting summary, one of the team members mentioned that we should go to the field to study the wastewater plant further understand the mainstream of the current market for wastewater treatment, and to find some inspiration for our team project. After tcommunication, we contacted the person in charge of the Jinshan sewage plant, who agreed to visit the plant and answer our questions The person in charge told us that the drainage system of Jinshan Wastewater Plant adopts a split flow system, and adopts a modified process of SBR - ICEAS wastewater treatment process , using the depth treatment process of " high efficiency sedimentation + filter cloth filter tank ", and the wastewater treatment reaches the standard of "urban wastewater treatment plant pollutant discharge standard" in Table 1 of Class A. After the initial understanding, we also told the person in charge of the principle and method of microalgae treatment of sewage. Microalgae have certain treatment effect on harmful sewage containing pesticides, antibiotics, heavy metals, etc., and have the effect of adsorbing toxic and harmful substances such as heavy metals within a certain concentration range. Our project is to modify the ability of microalgae to absorb N and P elements through transgenic technology and play a greater role in sewage treatment. He appreciated our project and mentioned that most of the wastewater plants have not yet used microalgae to treat wastewater, which is mainly due to the fear of high cost, unknown risks of the new technology, etc.

Government's Concern

According to the United Nations Sustainable Development Goal No. 6: Everyone should have the right to clean drinking water and water use, in order to ensure that more people understand the knowledge related to water conservation, water love and water protection, and to raise good awareness of resource conservation, our government has also formulated relevant policies to monitor the sewage data testing of factories, enterprises, sewage plants, etc., and adopted a transparent and open system so that the public has the right to know what social institutions have to say about sewage treatment programs. This will help to improve the quality of life of people and the natural ecological environment.

Help Received

Although the Jinshan sewage plant does not use the technology of microalgae to treat the sewage, the person in charge knows something about the method of microalgae treatment of sewage. After communication, he told us that pesticides, antibiotics, etc. can be degraded or adsorbed within the tolerance limit of microalgae. This point gave us a direction to think about, and for the adsorbed algae, we plan to study it again later to achieve pollution-free treatment through thermal cracking and other forms to further improve the project's treatment system.

Interview with Government Officials of Yangkou Town

Introduction

Yangkou Town, located in Shunchang County, Nanping City, has a history of more than a thousand years. Currently, is now recycling excrement jointly with enterprises to obtain more additional production value and promote zero-carbon industries. Huang Naigan, Minister of Yangkou Town Government, is an expert in applying environmental governance to practice. We were inspired by the story of the governance environment he conveyed, and his efforts to capture the audience's attention, so we decided to reach out to him for their feedback on the way we present our projects. We hope that through this interview, we can learn about the measures the local government has taken on the governance of the rural ecological environment, so that we can better understand what publicity we need to do to contribute to raising the public's awareness of environmental protection.

Interview with Naigan Huang

In July this year, we interviewed Mr. Huang Naigan, Minister of the Armed Forces of Yangkou Town, Nanping City, at the Yangkou Town Government. The subject of this interview is mainly about how Yuyangkou Town has taken the zero-carbon Shunchang concept as its foothold and realizes a series of innovative measures such as environmental governance and ecological protection. As far as we know, Shunchang County has been working hard for rural environmental governance in recent years. Huang Naigan also proposed: In order to implement the zero-carbon Shunchang concept. Yangkou Town's work on ecological governance has been implemented in all aspects of life, starting from the management of rural domestic waste and in accordance with "reduction first, encourage classification, urban and rural overall planning, and comprehensive management". Our team also thought of this in advance, so we put forward more in-depth discussions on the rural governance environment. After learning about our project, Mr. Huang Naigan now expresses his affirmation. He said that in the early stage of treatment, we were very troubled with sewage treatment. Many villagers would wash clothes by the creek, but the water quality was poor. It took a lot of energy and material resources. He said that our project can, to a certain extent, conduct popular science on water control for the masses. It can be seen from our active discussions on site that the government is very concerned about this aspect. During our communication, we talked about the need to strictly implement a series of measures such as the implementation of the management system in order to control the rural water environment.

Project Impact

We not only exchanged our experience in rural environmental governance with him, but he also gave positive comments on our educational activities over the past year. The focus is on popular science education for people in underdeveloped areas, and we take into account the scientific and environmental aspects of the project, as well as privacy. In his opinion, our interesting popular science propaganda has caught his attention. Regarding the method of popular science education, he strongly suggested that we use the form of art to popularize some incomprehensible synthetic biology knowledge. All in all, this interview brought fun to everyone and gave us new ideas on how to conduct educational activities.

Vision

First of all, thank you very much for having this opportunity to communicate with Mr. Huang Naigan. In this interview, we learned about Mr. Huang Naigan's measures to manage the rural environment. At the same time, our team also thought about how to apply our technology to less developed areas. , so we have a new direction, we hope to control the cost of our technology and the acceptance of new technology by people in underdeveloped areas as much as possible. This has promoted the progress of the project, and we have also been affirmed by Mr. Huang Naigan. We are very grateful for the project ideas he provided, and he also led us to understand the relevant policies, which is of great significance for us to put the laboratory results into practical production applications.

Online Teaching with Elementary School in Guangxi

Our Purpose

On October 9th of this year,We carried out a small class to support teaching to children in remote mountainous areas at Guangxi Laibin City Wuxuan County Experimental Junior High School , explaining to them the basic content and knowledge about synthetic biology, giving them an initial enlightenment about biology, which has a significant and far-reaching impact.

Process and Development

We took this opportunity to go into the mountain village and learn about the lack and scarcity of educational resources for the children in the mountainous area, and we also learned that the environment and conditions are very backward due to the geographical location and transportation. After understanding the situation, we wanted to help them learn more with our own power. So we started our teaching activities here. In the process, we educated the children about synthetic biology and briefly about our project. We not only talked about the origin of life, the cell, but also taught them about the life of a cell and that people are made of cells, and the children were very interested. At the same time, we showed them the hand-drawn book that we designed and drew ourselves and introduced them to our topic of microalgae through the hand-drawn book. The children were very curious about the biological knowledge and asked us questions, and we answered them patiently.

Good Wishes

After the popularization of science, we took a group photo with the children to record their thirsty faces for biological knowledge. At the same time, we also asked them what they wanted to know so that we could do the science fair next time. We to protect the water resources, advocate garbage classification as a starting point, hope can for high school students to preach about sewage treatment technology, the prevention of water pollution, garbage classification and so on, so as to make students form and strengthen the consciousness of environmental protection and garbage classification and form a relatively complete system of environmental protection and the related method, the important thought of the promotion of green environmental protection, Make garbage sorting and water resources protection a good trend and conscious action for more people.

Community Mobile Classroom

Presentation

We are very aware of the importance of reaching out to people associated with the discipline of synthetic biology and informing them about it. This year, we decided to communicate with our students on campus about our project in order to gain a comprehensive understanding of the impact, utility and nature of the project and the application of our proposed solution. The traditional teaching model is fixed in the room and limited by time, space, equipment, etc. We wanted to break this barrier in an easy way, so that students are not limited by the school LAN and geographical restrictions; break through the spatial restrictions, teaching and learning not necessarily indoors, but also outdoors. So we decided to set up a mobile classroom in Topside Square of Fujian Agriculture and Forestry University to promote our project through posters, video broadcasting, brochures, etc.

on Site

We noticed that some students in our school are relatively backward in their understanding of science knowledge, and we popularized science content as close to life as possible, such as the introduction of microalgae and the interpretation of gene editing. We believe that similar outdoor classes can inspire students to learn intensely and will be an unforgettable experience for everyone. In addition, we produced some interesting science cartoons, hand-drawn books and brochures on biological evolution, synthetic organisms, etc. For example, used microalgae to treat sewage as an example to tell students about the amazing effects of microalgae, so that students could understand that biology is not only limited to the visible range, but also the amazing microscopic organisms. At the same time, we distributed a paper version of our questionnaire to collect the knowledge of synthetic biology among the university students.

Reflection and Further Improvement

Our idea was well received by students as well as school teachers, who believed that the teaching content of modern education system should not be limited to traditional subjects such as language, mathematics and English, and that this kind of science popularization and competition could open up students' horizons and cultivate their interest in science, as well as contribute to the sustainable development of cultivating new STEM talents with the 2022CHINA-FAFU iGEM team's modest contribution.




Interviews with Experts: Including Prof.s from the School of Public Health, CDC Staff, and River Coast Researchers

Conference Background

On September 17, we conducted an online meeting with Guangxi University GXU-China and Nanjing Foreign Language School Nanjing_NFLS to interview experts together. At the same time, we formed the Fresh Freshwater Bioremediation Alliance (FBA). We jointly invited Professor Shen Xia from Nanjing Academy of Water Resources and asked him questions about wastewater management.

Conference in Progress

During the meeting, we asked Prof. Shen Xia about the governmental and private aspects of wastewater management, and she explained to us critically the channels and methods of wastewater management. Then, she introduced to us in detail the methods of sewage treatment, i.e. through physical, chemical and biological methods. The application of engineering bacteria is the biological method, in fact, it has two major advantages, first of all, it is a relatively low cost and no secondary pollution. In our national water treatment, including the treatment of sewage treatment plants, as well as the treatment of natural water bodies, all include the application of microorganisms. At the same time, Prof. Shen Xia also taught us the method of microbial treatment and the feasibility of machine treatment of sewage. We also consulted Prof. Shen Xia about our project - about the feasibility of microalgae treatment of sewage, and the professor fully affirmed the authenticity and feasibility of our project after understanding it, and encouraged us to continue our efforts towards the research direction.

Wishes and Prospects

At the end of the meeting, FBA had a relevant discussion, we deeply understood the current situation of sewage pollution and treatment, we gathered together to discuss each team's treatment and solution for sewage, and understood the situation of each of them. We each provided suggestions and plans for each other's development, which were approved and supported by everyone. With the development of science, the progress of the times, and the rapid growth of population, the water resources on which human beings depend are polluted and the amount of water we can drink and use is unknowingly decreasing. The issue of water recycling is gradually receiving widespread attention from the society, and the solution of a series of problems such as eutrophication of water bodies, excessive bacterial content and harmful residues is imminent. In implementing the 17 sustainability goals proposed by the United Nations, we also discussed the feasibility of microalgae treatment of wastewater technology in practical production applications in this conference, which provides experimental ideas for the laboratory and is of great significance for putting the laboratory results into practical production applications.