1 Executive Summary
1.1 Project Background
1.2 Team Overview
1.3 Product Technology
1.4 Market Analysis
1.4.1 Rockweed Flavoprotein
1.4.2 Eicosapentaenoic acid(EPA)
1.4.3 Biodiesel
1.5 Marketing Strategy
1.6 Corporate Strategy
1.7 Corporate Management
1.8 Financial Analysis
1.9 Risk analysis
2 Product Technology
2.1 Raw material advantage
2.2 Process advantage
2.3 Technical advantages
2.4 Product efficacy
2.4.1 Rockweed Flavoprotein
2.4.2 Eicosapentaenoic acid(EPA)
2.4.3 Biodiesel
3.2 Market Status Segmentation
3.2.1 Team Product Analysis
3.2.2 Rockweed Flavoprotein Market
3.2.3 Eicosapentaenoic Acid(EPA) Market
3.2.4 Biodiesel Market
3.2.5 Cooperating Companies
3.3 Market Size and Potential Analysis
3.3.1 Questionnaire Analysis and Modeling
3.3.2 Market Potential Analysis
3.4 SWOT Analysis
4 Marketing
4.1 Product strategy
4.2 Pricing strategy
4.3 Sales target
4.4 Promotion strategy
4.5 Sales Service
4.6 Marketing Management
5 Production management
5.1 Production requirements
5.2 Plant location and layout
5.3 Main production equipment
5.4 Production Technology Roadmap
5.5 Warehouse Management
5.5.1 Storage mode
5.5.2 Shelf life
5.5.3 Expired product handling
5.5.4 Fire safety measures
5.6 Product quality management
6 Company Management
6.1 Company Overview
6.2 Environmental Analysis
6.3 Team Management System
6.4 Company strategy
6.4.1 Initial Strategy
6.4.2 Mid-term strategy
6.4.3 Long-term strategy
6.4.4 Strategy Summary
6.5 Corporate Culture
6.6 Corporate Social Responsibility
7 Investment and Financial Analysis and Forecast
7.1 Financing Analysis
7.1.1 Capital requirements and flow of funds
7.1.2 Financing methods
7.1.3 Investor exit methods
7.2 Financial Analysis
7.2.1 Analysis and forecast of operating costs
7.2.2 Operating Revenue Forecast
7.2.3 Analysis of company's profit plan
7.2.4 Project investment benefit analysis
8 Risk Analysis and Control
8.1 Risk analysis
8.2 Risk response strategy
Born from the sea, to the sea and rise, "micro" to the new creation, "algae" has the action. The team of "micro" to create a new, "algae" have a role to play always adhering to the mission of "the use of marine resources, for the benefit of human health", adhere to the "a seaweed The team always adheres to the mission of "using marine resources to benefit human health", adheres to the development line of "one seaweed into a big health industry", adheres to the company philosophy of "people-oriented, leading in science and technology, serving agriculture and caring for people's livelihood", adheres to the development strategy of "developing green products and protecting ecological environment", and focuses on We always start from the realm of great love, practice social values, and devote ourselves to promote the sustainable development of ecological environment, which will last for a long time.
With China's dream in mind, we want to dream of deep algae. On the road of deep plowing blue marine economy, the team will not forget the original intention, forge ahead, take up the responsibility, dream of deep blue, and make new contributions to the cause of human health!
The project focuses on the microalgae industry, and always carries out the great goal and enterprise core concept of "developing energy-saving and environment-friendly ecological agriculture, building a sustainable and harmonious society", and is committed to building a famous brand of microalgae in China, so that "micro" can be newly created and "algae" can be the pioneer of "new era, algae health" low-carbon energy revolution. The "algae" has become the forerunner of the "new era, algae health" low-carbon energy revolution.
Marine diatoms are the main group of unicellular photosynthetic eukaryotes, widely involved in the biogeochemical cycle [1]. They are of interest because of their high photosynthetic efficiency, short growth cycle, and strong remediation effect on environmental eutrophication. With the growing world population, the use of microorganisms to establish cell factories to alleviate land and water scarcity is gradually gaining attention, and microalgae are expected to be developed as materials for low-cost mass production of clean energy and high nutritional value components, and have been widely used in basic science research and applied research [2]. As a typical single-celled marine diatom with high environmental adaptability, wide distribution, high bioactive components and high carbon sequestration capacity, brown finger algae of Triangulum is also expected to have broad application prospects in the fields of clean energy, carbon neutral and food and drug additives, and has become one of the hot research organisms for new energy and new food additives [3-4]. Studies have shown that 1.83 kg CO2 is required for the accumulation of each kg of microalgal biomass, and the use of microalgae to fix CO2 is expected to be one of the methods to mitigate the greenhouse effect in the future [5].
Carotenoids are carotenoids rich in marine brown algae, and their special chemical structure makes them useful in plant photoprotection, as well as in anti-inflammatory, antioxidant, anti-obesity, anti-diabetic, and in the treatment and prevention of various diseases, such as Alzheimer's disease [6-7], with important applications in biomedicine and functional foods. The marine diatom Trionyxus triangularis contains relatively high levels of lithoflavin and is considered as a new source of algae-based lithoflavin other than brown algae. Macroalgae are currently the main source of lithophanin, but their low content is only about 0.01% of their dry weight [8], which is one of the main reasons for limiting the production efficiency.
The lipid component of microalgae is influenced by various factors, and part of it can be converted into biodiesel, while another part, such as eicosapentaenoic acid (EPA), can be added as a nutraceutical product. ePA is a polyunsaturated fatty acid of the ω-3 series and an essential fatty acid that the human body cannot synthesize on its own. Some studies have shown that EPA has preventive and therapeutic effects on cardiovascular and cerebrovascular diseases, diabetes, and kidney diseases [9]. However, at present, most commercially produced EPAs come from deep-sea fish oil, whose processing and production process has irreversible effects on the marine ecological environment, and there are uncertainties such as marine pollution and resource depletion. As a kind of marine oil-producing diatom, the extraction of brown finger algae of the Triangle plays a positive role in carbon fixation and efficient utilization in the environment by using its extraction to produce EPA and biodiesel.
Biodiesel is the fatty acid methyl ester or ethyl ester formed by the esterification of vegetable oil, animal oil, waste grease or microbial grease and methanol or ethanol. Biodiesel is a typical "green" fuel, the best alternative to petroleum diesel, and one of the main directions of future energy development, with good environmental performance, good engine starting performance, good fuel performance, wide range of raw material sources, renewable and other characteristics [10-11]. Compared to conventional fossil diesel, biodiesel can significantly reduce exhaust emissions, and studies have shown that pure biodiesel has 46.7% less combustion carbon dioxide (CO) emissions, 66.7% less particulate matter emissions, and 45.2% less unburned hydrocarbons. Third-generation biomass using microalgae as the main feedstock has even more significant advantages due to its high yield per unit area, which can contribute positively to the realization of a carbon-neutral economy [12-13].
The accumulation of bioactive components in microalgae is influenced by many factors, such as the type of nitrogen source, nitrogen concentration, salt concentration, temperature and light intensity [14-15]. Achieving elevated lipid and lithophanin content in individual algal cells is currently mostly done by nutrient deficiencies or external environmental stress, but their total yield often decreases due to a decrease in biomass and does not reach the desired level [15]. In the study of antioxidant function in microalgae, ascorbic acid is considered to play a crucial role in the defense against photooxidative stress in all microalgae. It was found that the addition of ascorbic acid at appropriate concentrations under cadmium stress significantly increased the growth rate of S. cerevisiae, along with a significant increase in chlorophyll content [16]. So far, there is no established culture method by adding exogenous L-ascorbic acid that can increase the lithophanin content of microalgae without inhibiting their growth while promoting lipid accumulation.
Microalgae, as one of the known photosynthetic organisms with high carbon sequestration efficiency, have become a popular research area with great potential to achieve the goal of "carbon peaking and carbon neutrality". The team took Phaeodactylum tricornutum, a common single-cell oil-producing diatom in the ocean, as the starting point, and used synthetic biology, molecular biology, bioinformatics, biochemistry, pharmacology, metabolomics, transcriptomics, etc. to explore the linkage between microalgae lipid anabolism, microalgae high-value product anabolism and related key genes. The construction of engineered algal strains was carried out on this basis. At the same time, the completed strains will be combined with laboratory results for modeling and further screening of engineered algae suitable for large-scale plant applications.
The team has many years of research in the field of new energy and resources of microalgae, and some of the results have been published in international mainstream journals and applied for several national invention patents. The research results obtained by the team will provide a new impetus for China to cope with climate change, energy shortage and green low-carbon development; the research based on microalgae will implement the "big food concept" and build a controlled, safe and secure cell factory to provide efficient engineered algae strains based on new biomanufacturing technology. At the same time, we will work with domestic and international experts, scholars and entrepreneurs to promote the goal of carbon neutrality in China and the world.
Figure 1 "Micro" to create a new, "algae" as a team to develop the product technology performance.
The core technology of this project is the development of a culture method that can increase the content of algae and promote lipid synthesis, and the use of synthetic biology to build an engineered algae strain to achieve the increase of lipid and high economic value products of Fucus trituberculatus. The components with different polarity size, boiling point height and molecular weight size were extracted separately by CO2 supercritical extraction technique selectively.
1.4.1 Rockweed Flavoprotein
Laminarin has become one of the research hotspots in the field of natural products, and more and more reports have been made about it as its applications become more and more widespread. As one of the main active ingredients of various marine plants, the application of lithophanin is valued by the society, and it can be used as one of the nutritional and pharmaceutical ingredients for the prevention and treatment of chronic diseases [17].
The selling price of lithophanin in the domestic and international markets has gradually increased in recent years because of the high technical cost of its factory preparation. For example, the price of 5% lithophanin ranges from RMB 1908 to 2862/kg. 41.1 g of standard dissolved in 2.5 ml of ethanol solution has a market price of RMB 7080 [18]. At present, the global market size of lithophanin maintains an average annual growth rate of 2.47%, and the research and technological development regarding the purification, purification and rapid detection of lithophanin is the key to promote the application of lithophanin in food additives, health food and pharmaceuticals in China as soon as possible in the future.
The market size of lithophanin is likely to expand further in the future, with sales expected to reach RMB 821 million in 2022. global lithophanin revenue was approximately RMB 796 million in 2021 and is expected to reach RMB 885 million in 2028.
1.4.2 Eicosapentaenoic acid (EPA)
Eicosapentaenoic acid (EPA) has been widely used in health food products because of its significant role in delaying aging and preventing and treating many human diseases. However, the main commercial source of EPA is from deep-sea fish oil. The extraction of EPA from deep-sea fish oil has many instabilities in terms of odor, taste and product quality, as well as the presence of a large amount of other unsaturated fatty acids in fish oil, thus increasing the production cost of purified EPA, which has led to a high price of EPA pure products.
At present, the global deep-sea fish resources have been declining due to overfishing, and the production of fish oil cannot meet the increasing market demand. With a series of policies such as marine conservation, parties are exploring alternative sources of EPA for commercial production. Among them, marine microalgae have high EPA content, and in several foreign countries, such as Israel and Australia, microalgae are cultured on a large scale and used for the production of high purity EPA [19]. The price of EPA extracted from microalgae with purity greater than or equal to 98% is RMB 80,000/kg.
1.4.3 Biodiesel
Nowadays, China is a net importer of oil, and the domestic oil reserves are not enough, so it is impossible to meet the development needs by simply relying on imported oil. Since the 1970s, China has been transformed from an oil-exporting country to an oil-importing country, and the total annual import of oil has reached 70 million tons and is increasing at a rate of 6%. The development of new energy sources such as biodiesel is of great significance to solve the oil constraints.
At present, the market of biodiesel is in short supply, and with the gradual normalization of the epidemic and the recovery of traffic and shipping, its market demand will be further enlarged. 2021 since the price of biodiesel has risen all the way, from 7000 yuan/ton to 11000 yuan/ton today, an increase of more than 50%.
The development of biodiesel is suitable for the implementation of China's "sustainable development" strategy because of its three advantages of clean, safe and renewable, and it will promote the implementation of "carbon neutral and carbon peak" and other goals.
The marketing method adopts a combination of point and surface approach, focusing on terminal marketing, specifically refers to the early in-depth market research and technical guidance of the factory, the middle of communication with downstream product enterprises, publicity and promotion of the team's extract of algae flavonoids, eicosapentaenoic acid (EPA) and biodiesel and other extract materials, this approach is to enhance the recognition of our technology, on the one hand, can also enhance product awareness The post-innovation design.
Later on, we will design a smart microalgae bioreactor and cooperate with the government to install it at the end of the exhaust pipe of large buses, which can reduce the carbon dioxide in the exhaust gas through absorption by microalgae, and use the Internet of Things and Internet technology to monitor the growth of microalgae in the reactor in real time and save the site cost for the team to cultivate microalgae.
We will publicize through various media methods to improve the visibility of the technology and products, communicate with the team through experts and authoritative guidance to enhance the advancement of the technology, and expand the influence through advertising and marketing, online bidding ad placement, etc.
Under the dual pressure of economic growth and environmental protection, technological progress is the key to achieve the goals of economic growth and carbon reduction. The "double carbon" is a profound change, which will bring about huge changes in consumption and market, and is both an opportunity and a challenge, requiring the joint efforts of the whole society and even the whole mankind [20].
In response to the "double carbon" goal of carbon neutrality and carbon peaking, the team has incorporated the policy factors of ocean carbon sink, green technology, carbon tax and "big food concept" into the consideration of long-term development strategy, and strived to develop the production line of refined microalgae through the optimization of technology and process. In addition, we will strive to achieve the goal of "zero CO2 emission" in the industrial line of extracting raw materials such as algae flavin, eicosapentaenoic acid (EPA) and biodiesel through the optimization of technology and process development, and actively carry out the construction of green manufacturing system and build a complete system from green factory, green industrial park, green supply chain to green design products in response to the call of the national and local governments. We will respond to the call of national and local governments.
Focusing on microalgae industry, the team always carries out the great goal and enterprise core concept of "developing energy-saving and environment-friendly ecological agriculture and building a sustainable and harmonious society", and is committed to building a famous brand of microalgae in China, so that "micro" can be a new creation and "algae" can be a pioneer of the blue carbon energy revolution. "Algae" has become the forerunner of the "new era, algae health" blue carbon energy revolution.
Through a set of specific task allocation and completion mechanism to achieve the management of personnel and team building, a series of complete daily management system, financial system, salary system and leave system. It is of great significance to sort out the business process of the team and determine the responsibilities of each person's position, etc. At the same time, our team will continue to adjust and optimize our management system through specific practical effects, and strive to be people-oriented and lead team members to a higher platform.
The project will make full use of the project bank loan of nearly 800,000 RMB to meet the basic requirements of product research and development, such as agency cooperation and product research and development in the early stage. On the basis of product development, we will cooperate with other project business-related companies such as "Fujian Shenliu Group", so as to contact the market, and strive to occupy a certain market share, and prepare for the later stage of establishing companies, independent research and development, and opening factories, etc. Among them, since the production link is mainly represented by factories, the investment in construction in the preliminary investment is 0.00 million yuan, product development costs are expected to be 40.00 million yuan, accounting for 50% of the project's preliminary capital, labor costs are expected to be 20.00 million yuan, accounting for 25%, and other costs are expected to be 200,000 yuan, accounting for 25%.
The pre-operating income of the project includes main business income and other business income. The main business income is the income from the team relying on the unique culture technology of Brown Finger Algae Triangle with engineered algae species and extracting eicosapentaenoic acid (EPA), rock algae flavin, biodiesel, etc. from them, and the income from the sales of Brown Finger Algae Triangle extract; at present, the team has two patents under application, and when the application is successful, it will also generate patent licensing fees as part of the income source. Other business income refers to the income obtained from other daily activities other than the main business of each type of enterprise. Since the first year was the start-up stage, with large investment capital, relatively high costs and low total operating income, the net profit was -$223.2 million. As the team gradually stabilizes in the market and expands its target groups and cooperation channels, the net profit is on an upward trend, and it is expected that the net profit from team sales can reach 237,365,000 Yuan by the third year.
In the course of the team's operation, asset risk may arise due to ownership issues, financial risk due to insufficient working capital for expenses in daily operation or excessive credit accounts, management risk due to poor management, etc.
Based on all of the above, the team should establish a sound financial management system, monitor financial risks, improve asset utilization and minimize total inventory costs in order to avoid financial risks in the course of operation. At the same time to improve the capital structure, the team plans to introduce two to three venture capital companies into the stock in the next five years to diversify the risk. In addition, the team will establish an effective management mechanism and set up a professional financial management team to provide reasonable financial management for the team and the future company in order to avoid management risks to the maximum extent. It will also improve the accuracy of asset evaluation to make the ownership of intangible property clear and reduce asset risks.
As a typical marine diatom, marine brown finger algae culture does not require fresh water, does not occupy arable land, and is easy to cultivate; compared with large algae, its growth cycle is shorter and the content of high economic value components is higher; at the same time, as a model organism of diatom family, the sequencing of the genome of brown finger algae has been completed, which is easy to observe and analyze and regulate. It is worth mentioning that due to the existence of both C3 and C4 photosynthetic pathways, B. triangularis has high photosynthetic efficiency and high carbon sequestration capacity, which is crucial for achieving the zero carbon economy desired by the project.
The project uses microalgae and molecular biology technology to promote the development of biomass energy and algae-based high economic value products, promote the promotion and development of microalgae products through low biotoxicity, low emission or even zero emission culture methods, increase ecological carbon sinks, and strive to achieve carbon neutrality in the life cycle of microalgae culture utilization.
The supercritical carbon dioxide technology used in the material extraction stage is characterized by high efficiency, fast speed, good safety performance, low cost and easy separation of products. It can maintain the biological activity of the active ingredients better, and at the same time, because the whole process does not use organic solvents, so the extracts have no residual solvent, which also prevents the extraction process from being toxic to human beings and pollution to the environment.
The core technology of this project is the development of a culture method that can increase the content of fucoxanthin and promote lipid synthesis, which can increase the content of fucoxanthin and at the same time increase the lipid content of fucoxanthin, especially the content of eicosapentaenoic acid (EPA). The method is simple, fast, practical, efficient, safe and controllable, and can promote the accumulation of fucoxanthin and lipids in Fucus triangularis, which can be used in industrial production of biodiesel or as algae food additives in the food industry on a large scale. We have also constructed two engineered algae strains with high lipid and lutein content, which could bring more economic benefits in some regions while mitigating climate change.
2.3.1 Flavoprotein
Fig1.
Fig2.
Rockweed flavins have various physiological functions such as antidiabetic, antioxidant, anti-inflammatory, and anti-malarial activities, which can be easily developed as safe marine drugs and nutritional products [21]. Studies have shown that lithophanin may also affect the expression of genes related to lipid metabolism, which can assist in achieving weight loss to some extent [22-23]. Also, based on its function of inducing apoptosis, lithophanin is an effective method for cancer inhibition. The results of animal experiments have shown that lithophanin is effective in the prevention and treatment of some lifestyle-related diseases, such as cardiovascular diseases, fatty liver, etc [24-25]. Laminarin can be easily developed as a safe marine medicine and nutritional product.
2.3.2 Eicosapentaenoic acid (EPA)
EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) belong to the omega-3 fatty acid family, a class of fatty acids that humans cannot synthesize on their own. They promote the reduction of triglycerides and are beneficial to heart health, and the combination of DHA and EPA has the effect of protecting the eyes and improving the transmitter function of the retina.
Past studies have shown that EPA can treat autoimmune defects, promote a healthy circulatory system, and help with growth and development. It can have a positive effect on the treatment of lung disease, kidney disease, type II diabetes, colorectal ulcers and segmental ileitis. It may also help to lower cholesterol [26].
EPA and DHA have aiding brain cell development, anti-aging effects, improving blood circulation, lowering blood lipids, antagonizing allergic allergic reactions, preventing allergic dermatitis, bronchial asthma, and relieving rheumatoid arthritis; reducing hepatic neutral fat and preventing fatty liver; in recent years, scientists have found that it has anti-cancer effects and can effectively inhibit tumor metastasis, enhance the sensitivity of tumor cells to chemotherapy drugs, and Improve the cachexia condition of the body and prolong the survival time of the tumor-bearing body; it can lower blood sugar and relieve the symptoms of diabetes. It can be used to strengthen the brain and improve memory and thinking ability, and has certain curative effect on memory loss and senile dementia [27-28].
2.3.3 Biodiesel
Biodiesel is the fatty acid methyl ester or ethyl ester formed by the esterification of vegetable oil (such as rapeseed oil, soybean oil, peanut oil, corn oil, cotton seed oil, etc.), animal oil (such as fish oil, lard, beef oil, sheep oil, etc.), waste grease or microbial grease and methanol or ethanol. Biodiesel is a typical "green" fuel, the best alternative to petroleum diesel, with good environmental performance, good engine starting performance, good fuel performance, wide source of raw materials, renewable, highly degradable, etc. [29-30]. Compared to conventional fossil diesel, biodiesel can significantly reduce exhaust emissions, and studies have shown that pure biodiesel has 46.7% less combustion carbon dioxide (CO) emissions, 66.7% less particulate matter emissions, and 45.2% less unburned hydrocarbons.
With simple composition, abundant raw materials, no pollutants and degradable, and friendly to the environment, biodiesel is green as a renewable, clean and safe energy source. Microalgae grow mainly in land, lakes and oceans, and are a class of tiny algal groups whose morphology can only be discerned under a microscope. Microalgae are rich in proteins, algal proteins, cyanobacterial proteins, fatty acids and other substances, and their oil content can reach 20% to 80%, which can be used as the main raw material for biodiesel [31-32].
"The core of "micro" is the gospel of the earth's organisms - microalgae. It is the main creator of oxygen during the evolution of the earth, and is also one of the oldest surviving organisms in the world. At the same time, microalgae also have a strong carbon neutral ability, farming out one ton of microalgae can consume at least 1.8 tons of carbon dioxide. In the global environment of carbon reduction, microalgae farming industry has unlimited possibilities. Its derivatives are widely used in various fields, including: pharmaceutical field, food processing, health care nutrition and animal feed.
The team focuses on the extraction of raw materials, product development and commercialization of the microalgae industry. "We are a technical team focusing on the research and development, culture and production of microalgae industry technology. The core shortcoming of China's microalgae industry is the lack of its own technological barriers. The team visited experts in various fields to explore the practicability of interdisciplinary integration, and after several years of hard work, "Micro" came to a new creation, "Algae" has a microalgae research and development laboratory and a professional microalgae technology research and development team of more than 20 people, from algae species screening and cultivation The team has its own core technology from algae species selection and cultivation, culture production and oil application.
At present, the core technology of the team is a culture method to promote the accumulation of microalgae luteolin and lipid synthesis, which is simple and fast, practical, efficient, safe and controllable, and can promote the accumulation of algae luteolin and lipid content of brown finger algae, which can be used in industrial production of biodiesel or as algae food additives in the food industry on a large scale.
In the early stage of development, the team's priority is to produce and sell the raw materials for extracts of fucoxanthin, eicosapentaenoic acid (EPA) and biodiesel, etc. Through the technology developed by the team, the biomass per unit volume of fucoxanthin, EPA and biodiesel extracted from Fucus triangularis can be increased at low cost, quickly and safely, and the photosynthetic activity of Fucus triangularis can be improved, so that the raw material extraction can finally The team will provide the technical basis for the final "zero emission" goal of raw material extraction.
In the middle of the team's development, we will build downstream industries such as cosmetics, health food and new energy supply.
In the later stage, the team will design a smart microalgae bioreactor, cooperate with the government, provide services at gas stations, apply the integration of "Internet" + "Internet of Things" + "supply chain", and adopt the "sharing" model to promote the use of microalgae. The "sharing" model, the promotion of intelligent microalgae bioreactor, installed in the tail of the exhaust pipe of large buses, one can reduce the carbon dioxide in the exhaust gas through the absorption of microalgae, the second can use the Internet of things and Internet technology, real-time monitoring of the growth of microalgae in the reactor, saving the team to cultivate microalgae site costs.
Nowadays, the application of lithophanin in the food and drug and cosmetic industries is increasing, but its expensive selling price is limiting the development of related products. Laminarin is an important carotenoid resource with great potential for development in the food and pharmaceutical sectors. Currently, the production of lithophanin from macroalgae such as brown algae has limited capacity and high cost. Many microalgae are not only rich in luteolin, but also have high biomass yield with rapid growth, which is expected to be an ideal cell factory for commercial production of luteolin.
Fig3. Analysis of global revenue estimation of lithophanin
Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid obtained from brown finger algae of the Triangle, is one of the nutrients that the human body cannot produce on its own and is essential, with anti-inflammatory, antioxidant and vascular softening properties, which are useful in cardiovascular disease treatment, depression, weight loss and exercise. Because of its important effect in the treatment of cardiovascular diseases, it has the name of "vascular scavenger".
VASCEPA (EPA high-purity preparation) has been introduced in Boao Le Cheng International Medical Tourism Early Zone in Hainan, and the first prescription drug has been issued. Meanwhile, the new resource food certificate of Microcystis aeruginosa will be approved in April 2021, which means the domestic and global market of EPA will enter a period of high growth.
"Micro-algae has been committed to research and development of high purity algae-derived EPA products for many years, and has the first-mover advantage and technical barriers in the domestic market.
3.3.1 Biodiesel Market
The U.S. Energy Administration predicts that the future market size of clean fuels will maintain a growth rate of about 10%, and will double in size by 2022, i.e., a total value of $24 billion. The estimated commercial demand in the market at this time is $6 billion, which is a very significant amount. As traditional fossil energy sources are consumed and their market fades away, clean fuels, fuel cells will become the all-important alternative [33].
China's crude oil production showed positive growth in 2019, but crude oil imports also exceeded 500 million tons, up 10.1% year-on-year. According to statistics, China's demand for oil accounts for about 30% of the world's demand for oil, while the demand gap for coal in China will reach 360 million tons in 2040.
At present, there are two main factors restraining the algae products market, on the one hand, the culture cost of microalgae is high, accounting for 60%-80% of the cost of biodiesel. On the other hand, high value bioactive ingredients such as rock algae flavins are currently mainly from macroalgae, but the content is low only accounting for 0.01% of the dry weight of the organism left, but the market demand is large, there is an imbalance between supply and demand contradiction. The market demand for new bio-energy and algae-based bioactive ingredients is high, and there are no mature companies providing algae-based products in mainland China yet. China is the second largest oil consumer in the world, but due to various reasons, the current domestic biodiesel production is only 15% of the demand, the market gap is large, I believe that the green new energy industry will be driven by the double carbon target, and will be developed significantly.
3.3.2 Cooperative enterprises
Ltd., a national health food GMP certified enterprise, is a technology-based industrial company specializing in spirulina farming, research and development, production and sales integration. The company's subsidiaries are: Shenliu Spirulina Biological Research Institute, Lvsheng Biological Engineering Co. We can provide more than 200 tons of high quality Spirulina powder per year. Spirulina has been awarded as "Spirulina Project Unit of the United Nations South-South Cooperation Network Demonstration Base" by the United Nations South-South Cooperation Organization to carry out international research, product development, project matching and exchange of Spirulina. The company has passed the approval of the State Food and Drug Administration for two Spirulina health food products, including two health food approvals for Spirulina tablets and Spirulina capsules; Spirulina series food products have passed four food safety QS certifications; the company's production has passed the national health food GMP certification, ISO9001-2000 international certification, and HACCP international certification for food safety control points.
The Spirulina series products developed and produced by Shenliu Company include: Spirulina tablets, Spirulina capsules, broken wall Spirulina concentrate tablets, Spirulina protein powder, Spirulina nutritional breakfast, Spirulina tablets, Spirulina concentrate powder. Spirulina soft capsule, sport spirulina food, spirulina vermicelli, spirulina noodles, spirulina moon cake, etc. Spirulina series products have been popular in the market. The products are sold all over the country and exported to Hong Kong, Philippines, Russia, Japan, Taiwan and other countries and regions.
3.4.1 Questionnaire analysis and model building
The "micro" to create a new, "algae" as a science and technology team through the pre-placement of questionnaires for market research, and then combined with the market design to establish a mathematical model for analysis, to get more objective and accurate market data.
In the model part, the team has built three models. The team established a stagnant growth model based on the relationship between the growth quantity and growth trend of microalgae to study the growth curve of microalgae to analyze the best culture environment for brown finger algae of Triangle. After that, the team collected data on the cultivation cost, time cost, and labor cost of the plant to build a target planning model to predict the optimal microalgae cultivation time and the corresponding yield of the plant. In order to microalgae effectively reduce the total cost of plant production and provide some support for the economic feasibility of microalgae production of biodiesel, we explored the best site for urban cultivation of microalgae through DEA model, fully considered the environmental factors affecting the growth of microalgae such as rainfall, wastewater volume and sunlight, and studied the economic factors such as transportation cost and land cost, and deeply researched the most suitable cultivation sites among 31 provinces in China. microalgae cultivation in 31 provinces in China.
We established a user demand model based on the CRITIC weighting method through a questionnaire survey, based on user questionnaire data and Maslow's demand theory, to study the correlation between user acceptance of transgenic microalgae and its products and each demand level. Based on the user needs model, we analyze the acceptance of users, use K-means cluster analysis method to explore potential users, and deeply explore the value of five major potential users and their personalized characteristics.
3.4.1.1 Model 1: Microalgae growth model
Solution target
Fucus triangularis is a marine eukaryotic unicellular algae with fast growth rate, high lipid content and easy to culture. By analyzing the growth of Fucus triangularis under different ascorbic acid concentrations and using cell density, lipid density and lithophanin content as evaluation indexes, we study the growth of Fucus triangularis and analyze the optimal culture environment and the maximum growth rate of Fucus triangularis, as well as its corresponding time and maximum production volume.
Effect of ascorbic acid concentration on the growth of Fucus trituberculatus
With the change of culture time, the change of cell density content of Fucus trituberculatus is shown in Fig4.
Fig4. Microalgal density profile
Model solving
The above process was solved by MATLAB software, and the fitted curve can be obtained as shown in Fig. 2, the
Figure 5 shows that the density of Fucus triangularis basically conforms to the S-shaped growth curve, reaching the first peak on the 10th day, and then entering the plateau period. Ascorbic acid had a promoting effect on the growth of Fucus triangularis, and the promoting effect of ascorbic acid addition in group D was the most obvious, which obviously increased the growth density threshold of the algae.
From this, we can find the growth pattern of B. triangularis: B. triangularis basically showed J-shaped curve growth in the first 8 days, which was due to sufficient nutrition in the medium and low growth resistance. 8-10 days, the density of algae increased, the growth resistance became larger, and the growth trend gradually slowed down. On day 11, the maximum capacity was reached, and thereafter the growth curve fluctuated above and below the maximum capacity. There was no significant senescence of the algal bloom in one growth cycle.
Conclusion
According to the actual growth curve of microalgae and the model fitting curve, it is easy to find that the cultivation environment in group D can not only make the brown finger algae of Triangle grow better and extract more oil, but also facilitate the accumulation of lithophanin. Meanwhile, the best incubation time for microalgae was 13 days, and the maximum density that could be achieved was 8.23×106 units/ml.
3.4.1.2 Model 2: Cost Target Planning Model
Problem Background
As the global population is booming and humans are faced with increasing food and energy demand and decreasing fossil fuel reserves, the use of untapped organisms to support sustainable development is a very popular research direction at present. Microalgae, as a biological resource with high photosynthetic efficiency and adaptability to the environment, have great potential to address carbon emissions and provide new energy sources. However, the commercial application of microalgae is severely hindered by their high production cost, and it is a serious practical problem to reduce the production cost of microalgae so that they can play their role in the new energy era.
Therefore, by studying the main factors affecting the production cost of microalgae, we establish a target planning model based on the plant's cultivation cost and time cost, and find a balance between the minimum value of cost and the maximum value of production to arrive at the optimal algae cultivation time and the corresponding production and cost.
Fig5. Cost and profit variation curve
Model evaluation
Based on the above results, it is clear that adding the right amount of ascorbic acid can increase the biomass of algae and thus the economic efficiency of the plant. Compared to not adding any additive, the profit is expected to increase by 15.7% year-on-year. By observing the production curve of microalgae, it can also be found that the growth rate of microalgae can be effectively increased with the addition of ascorbic acid in group D. It can effectively promote the profit growth of the plant and improve the economic efficiency. While other additives did not promote the growth of microalgae and produced lower economic benefits than those without any additive.
Further sensitivity analysis of the experimental results of group D and without adding any additives showed that when the labor cost was less than $232, the profits obtained from the microalgae cultured in group D culture conditions were all greater than those without adding any additives. And when the labor cost is greater than $232, the profit of group D may be lower than the profit without adding any additives. This is because the microalgae in group D culture condition reached the maximum density on the 10th day, while the microalgae without any additives reached the maximum density on the 7th day. When the labor cost is larger, the time cost of production is also larger at this time, so there will be a situation that the profit of group D is lower than the profit without any additive. However, on the whole, as long as the labor cost is controlled and the production scale is expanded, group D can still increase the profit of the plant by increasing the production of microalgae thus effectively.
3.4.1.3 Model 3: DEA-based model for siting microalgae plants
Research Background
Microalgae have a high potential to contribute to world energy and mass commodity demand as a raw material for the production of energy and other commodities, but have always been inhibited by their production costs. After studying the optimization of plant production cost of microalgae, we select the best site for microalgae production by data envelopment analysis method for the geographical environment and economic situation of 31 provinces and regions in China. Optimizing the site for microalgae production can effectively reduce the total cost of plant production and provide some support for the economic feasibility of microalgae production of biodiesel.
Fig6. Site selection map of microalgae plant
Discussion of the results
In the results of data envelopment analysis, if the optimal solution of linear programming, the decision unit DEA is said to be valid, indicating that the input to output ratio is optimal, if, then the decision unit non-DEA is said to be valid, indicating that the input to output ratio is not optimal, generally speaking, the larger theta indicates the better effect.
We solved the model by python and obtained the evaluation results of 31 provinces as detailed in Appendix I. Therefore, synthesizing the above results, we concluded that when selecting sites for the production of microalgae in 31 provinces in China, the seven provinces of Shanghai, Jilin, Jiangsu, Sichuan, Xinjiang, Guangxi, and Shaanxi are the most suitable sites for cultivating microalgae in China, while the provinces of Heilongjiang, Tianjin, Henan, and Shandong are not suitable for cultivating microalgae.
The questionnaire analysis and the specific model data processing are detailed in Appendix I.
3.4.2 Market potential analysis
The current situation of algae culture in Fujian Province is shown in the figure below, and the national production of marine aquaculture algae grew from 1.962 million tons to 2.414 million tons from 2014 to 2019, and its annual growth rate increased year by year, from 2.6% in 2015 to 7.1% in 2019, with an average annual growth rate of 4.2%. Compared with the national production of marine shellfish culture in the same period,
the production of marine algae culture maintained a higher growth rate. In addition, kelp culture dominates the total production, with its annual average share of production up to 68.9%, but its share of total production decreases slightly year by year, from 70.0% in 2015 to 67.3% in 2019.
It is worth mentioning that the farming share of Gracilaria japonica is slightly increasing year by year, from 13.4% in 2014 to 14.4% in 2019, and its average annual growth rate can be 5.8%. From 2014 to 2019, the production of marine algae culture in Fujian Province maintained a high growth rate [Fig. (b)], from 796,000 tons to 1,135,000 tons, with an average annual growth rate of 7.3%, and even reached 9.7% in 2016, which was significantly higher than the national growth level of marine algae culture in the same period.
Similar to the national seawater algae culture, kelp culture dominates the seawater algae culture in Fujian Province, and its average annual production accounts for about 73.3% of the total production of seawater algae culture in Fujian Province. And kelp culture production is increasing year by year, from 60.0 thousand tons in 2014 to 803 thousand tons in 2019, with an average annual growth rate of 6.0%.
In addition, seawater algae culture in Fujian Province accounts for more than 40% of the total national culture production, indicating that the contribution of seawater algae culture in Fujian Province is clearly in a dominant position in China. Moreover, the share of marine algae culture in Fujian Province in the total national production increased year by year from 40.56% in 2014 to 47.02% in 2019, indicating that marine algae culture in Fujian Province is developing rapidly.
Fig7. (a) Production of mariculture algae from 2014 to 2019
(b) The percentage content in (b) is the proportion of algae culture production in Fujian Province to the national production
Eicosapentaenoic acid (EPA) intake alone (without the addition of DHA) is also significant for human cardiovascular health and in the treatment of related diseases. In the nutraceutical market, the demand for plant-based EPA and DHA for vegetarians is growing at a relatively high rate.
At present, the product application development direction includes functional blended oil, vegetarian microencapsulated powder, vegetarian soft capsule, tablet, punch, high purity API, etc. The corresponding process and equipment development is also in progress. With the deepening of related research and the gradual market launch of products, it is believed that EPA extracted from microalgae will be more and more widely used in food and pharmaceutical fields [34].
With the growth of expectations for sustainable green energy and the development of economy and society, biodiesel has received widespread attention and is the main direction of future energy development because of its good fuel performance, environmental performance, good engine starting performance, wide range of raw material sources, and renewable properties. As an alternative energy source, life cycle analysis is used to evaluate the energy and environmental impacts of biodiesel produced from cultivated soybeans, corn ethanol, gutter oil, kohlrabi fruit oil and microalgae, among which biodiesel produced from microalgae is of considerable interest.
Microalgae can convert water and CO2 into O2 and organic macromolecules through photosynthesis, and the oil from them can be extracted and converted to produce high quality microalgae biodiesel. Microalgae are considered as a potential alternative energy source because of their high photosynthetic efficiency, short growth cycle, high yield and quality of oil and grease, good adaptability, and no occupation of arable land [35].
The third generation of biodiesel using microalgae as the main raw material has even more significant advantages due to its high yield per unit area. China has 150 million mu of saline land. Under the condition of mature technology, if 14% of saline land is used to cultivate microalgae, the amount of diesel produced can meet 50% of the national oil demand. The production scale of biodiesel can reach tens of millions of tons with large-scale cultivation of engineered high-oil algae in the vast coastal and inland waters of China [36].
SO Strategy.
With the support of national policies, improve the products and increase the innovation and safety of the products
WO strategy.
Actively cooperate with related industries with the help of the government to ensure sufficient funds. Improve the experimental technology and master the core technology in key areas
ST Strategy.
Increase the publicity of product technology and improve public relations ability. Take advantage of technological innovation and continuously increase product market share
WT Strategy.
Refine experimental technology and master core technology; increase public relations and publicity to enhance product awareness
Ltd. as the technology provider, the team will borrow the microalgae plant of Fujian Shenliu Health Food Co., Ltd. to cultivate microalgae and extract rock algae flavin, EPA, biodiesel and other raw materials.
In the second quarter of the third year, with the previous technology fee as the start-up capital, the team built its own plant and cultivated microalgae, extracted and sold rockweed flavin, EPA, biodiesel and other raw materials.
In the middle stage, the team started to expand downstream industrial lines, such as cosmetics containing luteolin, health products containing EPA, and sales of biodiesel.
At the later stage, the team innovated and designed a smart microalgae bioreactor, which was installed at the end of the exhaust pipe of a large bus in cooperation with the government, to reduce the carbon dioxide in the exhaust gas through absorption by microalgae, and to use "Internet of Things" and "Internet" technology to combine with the "supply chain" to provide a real-time supply chain. "The supply chain can monitor the growth of microalgae in the reactor in real time and save the cost of the site for the team to cultivate microalgae.
Our raw materials are divided into three types: rock algae flavin extract, eicosapentaenoic acid (EPA) extract, and biodiesel extract. Our product pricing will refer to the market price to set the product price.
The price of 5% rockweed flavin is RMB 1908~2862/kg. 41.1g of standard dissolved in 2.5ml of ethanol solution, the market price is RMB 7080. So our Rockweed Flavoprotein extract products are priced at 1908~2500 RMB/kg.
The price of eicosapentaenoic acid (EPA) extracted from microalgae with purity greater than or equal to 98% is RMB 80,000/kg. our EPA extract is priced at RMB 75,000/kg.
The market price of biodiesel is $11,000/ton, and our biodiesel extract is priced at $10,000/ton.
Daily prices will be adjusted in real time following the fluctuation of the market.
The team's customer type is specifically oriented to cosmetic companies containing rockweed flavonoids, health care companies containing eicosapentaenoic acid (EPA), new energy companies using biodiesel, and cooperation with government
Research findings.
Users' demand for microalgae and their products mainly comes from safety needs and emotional needs.
When tapping potential users, companies need to focus on middle-aged and elderly people who are highly educated or experienced.
Pre-marketing price strategy:
In order to quickly open the market and gain a certain market share, we will use 20% purchase discount for new customers in the early stage, so as to attract new customers to deal through preferential way, enhance the order closing rate, improve the market share of the product, and play the purpose of accumulating customers and helping the company to obtain cash flow quickly.
The first phase of product sales take bulk wholesale mode:
As the initial team is to do is the source of the product factory, will not spend a lot of energy to build a retail network, so our product sales model will be the wholesale of raw materials products, wholesale products to downstream enterprises, such as cosmetics containing rock algae flavin enterprises, health products containing EPA enterprises, new energy enterprises and other bulk customers.
In the middle and later stages of product sales, we will adopt the cooperation between government and enterprises, "Internet" + "Internet of things" + "supply chain", and "shared ecological The "shared ecology" mode of operation.
The team innovates and designs the intelligent microalgae bioreactor, and cooperates with the government, and plans to put it in gas stations to be installed at the end of the exhaust pipe of large buses. The social benefits of the device are first, to reduce the carbon dioxide emitted into the external environment from the exhaust through absorption by microalgae, and second, to save the team's site costs for cultivating microalgae.
The team plans to: first, design sensors in the device, which can detect the light condition, carbon dioxide absorption rate and other indicators in the device; second, design positioning software, so that microalgae strains that follow the bus, so that the destination of microalgae can be traced; third, the Internet of Things and Internet technology can be used to monitor the growth of microalgae in the reactor in real time, so that the source of microalgae can be traced; fourth, develop the " Fourth, develop a "shared ecology" model, in which the bus unloads the cultivated microalgae in the microalgae biosensor to the gas station periodically and replaces it with a new microalgae biosensor, assigning a code to the smart microalgae bioreactor and adopting the sharing mode of scanning the code to serve the public intelligently.
Fig8. The left picture shows the exhaust emission without the smart microalgae bioreactor.
The right diagram shows the improvement of tail gas emission after the installation of smart microalgae bioreactor.
Fig9. shows the internal design of the smart microalgae bioreactor
The interior of the Smart Microalgae Bioreactor device is initially planned to consist of a spiral tube (to reduce the exhaust gas flow rate), a desulfurization and denitrogenation membrane (to treat SO2 and NO2 in the exhaust gas) and a microalgae absorption chamber. Together with sensors and other chips, it will achieve the goals of standardization of emission process, efficiency of tail gas treatment and wisdom of service.
Marketing is targeted through four modes: government-enterprise cooperation mode, direct sales mode, joint sales mode and customized mode.
In particular, according to the third phase of the plan, in order to promote the public to accept the team's intelligent microalgae bioreactor products, we plan to adopt the "fuel price full reduction" strategy, that is, the use of a certain number of replacement and recovery of intelligent microalgae bioreactor, a certain amount of microalgae for the team to cultivate, can enjoy a certain amount of fuel price discount when refueling at gas stations! The team can enjoy a certain amount of discounted gas prices at gas stations.
Production cycle: The best culture cycle of microalgae is 11 days as shown by the analysis of cost target planning model.
Worker requirements: college or university degree in related field, three months of professional training.
Technical key: algae culture technology, mastering the production operation of oil and algae flavin.
In recent years, the production of biodiesel from microalgae has attracted much interest. The advantages of urban cultivation of microalgae are urban wastewater use, oxygen production and employment sustainability, and the commercialization of biodiesel production using microalgae is supported by scientific evaluation and practical experience. We identified the most suitable provinces for cultivating microalgae in 31 provinces in China by DEA model, fully considered the environmental factors affecting the growth of microalgae such as rainfall, wastewater volume and sunlight, and studied the economic factors such as transportation cost and land cost, and the obtained results can effectively reduce the raw material cost of diesel production, and also verified the validity and In the next step, we will continue to study the location of the microalgae plant in more detail.
From the results of the analysis in 3.3.1, we concluded that in the 31 provinces of China for the site selection of the production origin of microalgae, the seven provinces of Shanghai, Jilin, Jiangsu, Sichuan, Xinjiang, Guangxi, and Shaanxi are the most suitable locations for cultivating microalgae in China, while the provinces of Heilongjiang, Tianjin, Henan, and Shandong are not suitable for cultivating microalgae.
The specific plant construction layout and costs are shown in the following table.
Table1
The team will initially pilot the cooperation in Fujian Province, and then migrate to Guangxi Province to cultivate microalgae in the middle of the company's development.
Table 2 List of main equipment
As shown in Table 2, the list of major equipment for our team.
Fig10. Process flow diagram
The production technology route for our team is shown in Figure 12.
5.5.1 Storage mode
Biodiesel: stored in the form of storage tank (vertical cylindrical metal oil tank)
Rockweed Flavoprotein: 1kg aluminum foil vacuum bag/25kg cardboard drum; stored in dry and cool place (low temperature), avoid sunlight and high temperature.
Eicosapentaenoic acid (EPA) particles: EPA is dispersed in vegetable oil, microencapsulated in carbohydrates, and produced by fluidized bed cold air drying method to produce microencapsulated spherical particles with excellent fluidity. It is stored in cans, packed in cartons, dry, protected from light and stored at low temperature.
5.5.2 Shelf life
Biodiesel: Theoretically, it can be kept for at least 1 year. (synthetic antioxidants can be added subsequently)
Eicosapentaenoic acid (EPA): 24 months (unopened in original package below 15℃)
Rockweed Flavin: 3 years at -20℃; 2 years at 4℃
5.5.3 Expired product disposal
Biodiesel is an extremely flammable substance, which can easily cause fire, so expired products should be recycled and sealed in containers for preservation, not to be dumped at will. After settling for a period of time, it can be used to clean the parts. A small amount can be stored properly. If the amount is very large, you can contact a company that specializes in recycling waste oil to sell it and increase revenue for the company.
The expired algin and eicosapentaenoic acid (EPA) will be collected and given to the garbage collection department for high temperature incineration.
5.5.4 Fire safety measures
The fire fighting of pure biodiesel and EPA can be done with dry powder, foam, carbon dioxide, or direct water spray, but do not let the burning liquid splash and spread. As biodiesel is flammable, it should be kept away from oxidizer, overheated environment and open flame source.
Rockweed Flavin is a powdered combustible solid, not only has the risk of fire, but also once it is suspended in the air and mixed with air evenly and reaches a certain concentration range, it will explode when it meets the fire source. We need to pay attention to keep the warehouse clean and keep a relatively dust-free environment and away from fire sources.
5.6.1 Product quality control of biodiesel
China national standard of biodiesel (BD100) - "Biodiesel for Diesel Fuel Blending (BD100) GB/T 20828-2007" has been implemented since May 1, 2007. The biodiesel produced by our company must meet the requirements of GB/T 20828-2007 before the products can be sold.
5.6.2 Product quality control of algae flavonoid
Homogeneity test (HPLC-UV method)
According to GB/T 15000.3-2008, the homogeneity test of the purity of the standard sample of rockweed flavin is carried out by single-factor multi-level test ANOVA method, and the sample is repeatedly measured three times by HPLC-UV method (high performance liquid chromatography-UV detection). The assay data were analyzed by ANOVA.
Stability test
In order to investigate the stability of the samples and determine the expiration date, the standard samples were taken to simulate the market packaging and subjected to a long-term test for 24 months at -18℃. Samples were taken and tested at 0, 6, 12, 18 and 24 months, respectively, to investigate the stability of the samples by examining the changes in the purity of the standard samples. The stability testing method was the same as the homogeneity testing method, both using the HPLC-UV method with two consecutive injections of each sample, and then the average value of its purity was obtained by the normalization method [37].
Other detection standards
Table 3 National standard numbers and corresponding items of other testing standards
Focusing on microalgae industry, the team always carries out the great goal and enterprise core concept of "developing energy-saving and environment-friendly ecological agriculture and building a sustainable and harmonious society", and is committed to building a famous brand of microalgae in China, so that "micro" can be a new creation and "algae" can be a pioneer of the blue carbon energy revolution. "Algae" has become the forerunner of "new era, algae health" blue carbon energy revolution.
The team relies on the Strait Joint Research Institute of Fujian Agriculture and Forestry University. In terms of research hardware, the institute has a laboratory area of more than 10,000 square meters, with the second and third generation of high-throughput gene sequencers, ultra-high resolution and ultra-high sensitivity laser confocal microscopes, mass spectrometers, single-molecule genomic imaging systems and other large precision instruments, and has established an open research platform including genomics, proteomics, bioinformatics, metabolomics, cell We have established an open research platform including genomics, proteomics, bioinformatics, metabolomics, and cell biology.
With outstanding research talents and perfect hardware and software research and teaching platforms, the Joint Research Institute of the Taiwan Strait has become a pilot base for domestic and foreign cooperation, a demonstration base for joint training of high-level talents, a pioneer base for scientific and technological innovation and transformation of scientific research results in the West China Sea, and a cradle for cultivating scientific and innovative talents.
The Strait Joint Research Institute is committed to cultivating master and doctoral students with rigorous scientific attitudes and mastering skills in core fields, aiming to create high-level composite innovative talents.
Under the call of the times of mass entrepreneurship and innovation, our team always upholds the original intention of innovation and entrepreneurship, and serves the society with our feet on the ground, practicing our original intention and mission with practical actions.
The above diagram shows the organizational structure and functions of the team. Through a set of specific task assignment-completion mechanism to achieve the management of personnel and team building, a series of complete daily management system, financial system, salary system and leave system are formulated. It is of great significance to sort out the business process of the team and determine the responsibilities of each person's position. At the same time, our team will continue to adjust and optimize our management system through specific practical effects, and strive to be people-oriented and lead team members to a higher platform.
6.4.1 Initial Strategy
Ltd. to cultivate microalgae and extract raw materials such as rock algae flavin, eicosapentaenoic acid (EPA) and biodiesel.
6.4.2 Medium-term strategy
In the second quarter of the third year, the team will acquire shares in Fujian Shenliu Health Food Co., Ltd. and build a factory to cultivate microalgae and extract and sell raw materials such as rock algae flavin, eicosapentaenoic acid (EPA) and biodiesel.
6.4.3 Long-term strategy
The team starts to expand the downstream industrial lines in the middle term, such as cosmetics containing rock algae flavonoids, health products containing eicosapentaenoic acid (EPA), and sales of biodiesel.
In the later stage, the team will design intelligent microalgae bioreactors, cooperate with the government, provide services at gas stations, apply the integration of "Internet" + "Internet of Things" + "supply chain", and adopt "The smart microalgae bioreactor can be installed at the end of the exhaust pipe of large buses to reduce the carbon dioxide in the exhaust gas through absorption by microalgae, and to monitor the growth of microalgae in the reactor in real time by using Internet of Things and Internet technology to save the site cost of cultivating microalgae by the team.
6.4.4 Strategy Summary
The team relies on seaweed as a strategic resource to develop seaweed active substances in depth, and then develop marine animal and marine microbial resources for the benefit of human health.
Ultimate Pursuit
Higher standard of product pursuit, more extreme user experience
Staying focused
Focus on microalgae industry, focus on technology research and development, focus on product innovation
Focus on the environment
Focus on the environment and nature to create a more friendly, healthy and green development and production model
Open and tolerant
Encourage differences and voices, respect the region and culture, and coexist with multiple values
Born from the sea, we are developing towards the sea, "micro" for new creation, "algae" for action.
For the benefit of the cultivator, for the benefit of the eater, for the national glory, for the national role.
The team of "micro" to create a new, "algae" has always adhered to the "use of marine resources for the benefit of human health" mission, adhere to the "a seaweed The team always adheres to the mission of "using marine resources to benefit human health", adheres to the development line of "one seaweed into a big health industry", adheres to the company philosophy of "people-oriented, leading in science and technology, serving agriculture and caring for people's livelihood", adheres to the development strategy of "developing green products and protecting ecological environment", and focuses on We always start from the realm of great love, practice social values, and devote ourselves to promoting the sustainable development of ecological environment, which is always new.
We are committed to promoting the sustainable development of ecological environment. "Micro" to new innovation, "algae" as a science and technology team will target the frontier of agricultural high-tech, innovative products, service to people's livelihood. With the mission of "rooting in the fertile soil of China for the benefit of future generations", we will light the fire of scientific development and illuminate the road of agricultural biology, and always stand at the tide of agricultural bioengineering technology, striding forward with our heads held high and moving forward. The "micro" to new creation, "algae" as a team has always inherited the spirit of craftsmanship, committed to the road of scientific and technological innovation, relying on innovation-driven, and constantly stretch the marine high-tech industry chain, the development and growth of emerging blue industry, to achieve the optimization and upgrading of industrial structure and enterprise transformation. Development.
Looking to the future, the grand goal of a strong ocean state and the beautiful blueprint of a healthy China have given the team a valuable opportunity to leap forward again. The team will practice the core value of "forgetting self-interest, cooperating and coexisting", use the same broad-mindedness and broad perspective as the sea, look forward to a longer-term goal, place the development of the enterprise in the national strategy and global coordinates, continue to practice the supply-side structural reform, strive to be the leader of the transformation of the old and new dynamics, and promote the sustainable and high-quality development of the enterprise. High-quality development.
With China's dream in mind, we will think about the dream of deep algae. On the road of deep plowing blue marine economy, the team will not forget the original intention, forge ahead, take up the responsibility, dream of deep blue, and make new contributions to the cause of human health!
7.1.1 Capital requirements and flow of funds
According to the whole operation needs of this project, in the middle of the project reaching steady operation, the team plans to raise 10 million RMB.
The main use of funds: factory construction, equipment purchase, personnel salary, product development, etc.
Table 4 Table of expected use of financing amount
7.1.2 Financing methods
(1) The way of raising this project is mainly in cash. After the investor injects funds through this team, the proportion of equity held by the investor in the company will be determined according to the investor's investment.
(2) A special account will be established for the investor's funds. If necessary, the funds raised can be placed in the custody of a third party trusted by both parties to ensure the exclusive use of the funds. Upon the arrival of the new shareholders' funds, the same team will hold a shareholders' meeting and sign an equity transfer agreement to clarify the shareholders' responsibilities and rights, as well as the proportion of the new shareholders' equity.
(3) The equity transferred by the fund raising shall not exceed the absolute majority of the equity held by the team, ensuring two-thirds of the voting rights of the core members of the team or the status of the actual controller.
(4) The investor shall not draw back the funds within 2 years after investing the first installment, and shall complete each installment as scheduled in accordance with the equity participation agreement.
(5) The investor enjoys all the rights of a shareholder and may send personnel to participate in the management of the team and supervise the operation of the project. The investor shall enjoy the dividends of the project in accordance with the shareholding agreement, and at the same time bear the losses in proportion to the capital contribution and assume limited liability.
7.1.3 Investor exit methods
Initial public offering, M&A, buyback, liquidation, etc. There are great differences in the level of capital return and riskiness of different exit methods. Regarding the exit method of venture capital, we envisage repurchase.
Specific scenarios for repurchase.
Given that an IPO is not possible, venture capital is considered to be launched in the form of management buyback and employee buyback. Management buyback is an initiative taken by the company's management to buy back the Company's shares in the hands of venture capitalists at the time of the venture capital launch in order not to have the shares owned by other companies. Employee buyback is when the employees of the company collectively acquire and hold the shares held by the venture capitalist. Employee buybacks are more motivating for the company's employees than other buyback methods, and venture capitalists can transfer some of their equity to achieve a partial launch of venture capital.
Management buybacks result in management holding a majority or even all of the company's equity, and management's interests are closely related to the company's interests, when synergies dominate. In management buybacks, on the other hand, management is buying the equity of the venture capitalist through a bank loan. The increase in corporate debt ratio also stimulates management to work hard. Therefore, this approach also serves as an incentive for management when management buybacks have been implemented initially.
7.2.1 Analysis and Forecast of Operating Costs
The main operating cost is the cost incurred by the enterprise for recurring activities such as selling goods and providing labor services. Other operating costs are expenses incurred in operating activities other than the main business activities recognized by the enterprise.
This project will make full use of the direct project application cost of $642,600,000, mainly for product development, to meet the basic requirements of agency cooperation, assets, employees, and so on in the early stage. On the basis of product development, the project will cooperate with other companies related to the project business, such as "Fujian Shenliu Group", so as to get in touch with the market and capture a certain market share, and prepare for the establishment of a company, independent research and development, and the opening of a factory at a later stage (the capital investment structure of the project in the early stage is shown in Table 5).
Table 5 Preliminary capital investment structure
7.2.2 Business revenue forecast
Operating income includes main business income and other business income. The main business income is the income from the team's unique Brown Finger Algae Triangle culture technology and the extraction of eicosapentaenoic acid (EPA), rock algae flavin and biodiesel from it, the income from the sales of Brown Finger Algae Triangle extract, and the future patent licensing fees; other business income is the income from other daily activities other than the main business of each type of enterprise (business income forecast as shown in Table 6).
Table 6 Business Revenue Forecast Table
7.2.3 Analysis of the company's profit plan
As the first year is the start-up stage, the investment capital is large, the cost is relatively high, the total operating income is small, therefore, the net profit is small and even negative, as the team gradually stabilizes in the market, expands the target group, the total operating income increases at the same time, the cost also gradually accounts for a relatively small amount, the team starts to make profit, the net profit margin is on the rise (the project revenue forecast is shown in Table 7).
Table 7 Revenue Forecast Table
7.2.4 Project investment benefit analysis
Investment payback period
Through the net cash flow, discount rate, investment amount and other data calculated by interpolation method, the payback period is about 1 year, and the investment program is feasible. Payback period = cumulative NPV years-1 + (unrecovered cash / present value of the year).
Project sensitivity analysis
The company has uncertainties from various aspects in sales revenue, investment and operating costs, and this team does sensitivity analysis for the three according to the unit factor changes of 10% increase and 10% decrease. Calculation of the return on investment and embedded payback can be seen: the team is most sensitive to the increase and decrease in sales revenue, operating costs are the next most reliable.
Return on Investment
According to the forecast of the team's operation in the next few years, the team can maintain a high profit growth and proposes to withdraw a reasonable proportion of funds from net profit as shareholders' return. For this purpose, no dividends will be paid in the first year and 30% of the net profit will be paid annually after the second year.
Asset risk
The issue of ownership seems to be simple, but in fact hides great risks. Efforts are made to improve the accuracy of asset billing, improve the degree of inventory realizability, improve the accuracy of asset valuation, make the ownership of intangible assets clear, and clarify the share allocation and management structure of the team (a company will be established in the future, hereinafter referred to as the company) to reduce asset risk.
Financial risks
Risks in the business process: Insufficient working capital for expenses in the daily operation of the team (company), too many loans resulting in heavy interest burden, too many credit accounts that cannot be collected in time, and improper profit distribution will seriously affect the normal operation of the team (company) and even lead to bankruptcy.
Management Risk
The management operation process affects the level of management due to information asymmetry, mismanagement, error in judgment, etc., which leads to the departure of personnel, poor team execution and other problems. We strive to improve the quality of managers, improve the organizational structure, improve the corporate culture, and standardize the management process to minimize management risks.
Venture capital exit risk, the team (company) in the process of operation, venture capital in various forms of exit and lead to turnover failure. Coping strategies are as follows:
Establish a sound financial management system to monitor financial risks, strengthen the construction of internal accounting control system, establish a sound authorization system, budget system, incompatible position separation system, internal reporting system, electronic information management system, risk warning system and a series of financial monitoring system to prevent and monitor various potential risks such as guarantee business.
Strengthen the internal financial management of enterprises, improve the efficiency of asset use and reduce the risk of business operation Strengthen the internal management of enterprises, optimize the structure of assets, reduce unreasonable use of funds and improve the efficiency of fund use in the following three aspects.
(1) Physical asset management: Many enterprise managers attach great importance to capital management, but neglect the management of physical assets, so the management of physical assets has become a soft spot for financial risk management. For inventory, it is necessary to minimize the total inventory cost while meeting the production and sales requirements. As for fixed assets, we should establish an accounting system for fixed assets and make reasonable depreciation and provisions.
(2) Money fund management: poor management of money funds will lead to idle funds and insufficient funds. If funds are idle, it means that they are not properly utilized and fail to preserve their value; if they are insufficient, the enterprise cannot pay the much-needed funds, which causes liquidity risk. Therefore, while improving the capital structure and making proper use of funds, a portion of funds should be set aside for daily operations to ensure the normal operation of the enterprise.
(3) Accounts receivable management: Poor control of accounts receivable can lead to serious loss of capital. The enterprise will strengthen the prevention and monitoring of accounts receivable, establish an enterprise accounts receivable management system, assess the financial and credit status of customers, sign contracts carefully, closely track repayments, and formulate collection plans and measures to reduce the occurrence of bad debts.
Strengthen the awareness of financial risk prevention and establish the correct concept of financial risk. All departments and employees of the enterprise should strengthen the awareness of risk prevention, especially the management of the enterprise and the employees of the finance department, and the concept of risk prevention should be carried out in every aspect of the production and operation of the enterprise and put into practice. Financial personnel should have solid knowledge of accounting, do a good job of collecting, organizing and analyzing financial information, make predictions and assessments of potential financial risks, and deal with and solve the financial risks that have occurred.
Establish effective management mechanism to reduce enterprise financial risks. Enterprises should also strengthen managers' awareness of financial risk prevention, make scientific decisions, set up a professional financial management team and establish a perfect financial risk management mechanism.
Optimize financing structure and reduce financing risks. An excessively high gearing ratio can easily trigger the financial risk of the enterprise. Therefore, this enterprise takes this factor into full consideration when raising funds, and strictly controls the asset-liability ratio to avoid the financial risks caused by debt financing is a crucial part of solving the enterprise's financial crisis.
According to the actual situation, look for diversified financing channels to ensure the availability of funds; use various channels to find well-known enterprises to guarantee loans for the company; and actively seek support from the agricultural sector fund.
Develop a reasonable marketing strategy, strengthen the management of accounts receivable, and make a reasonable forecast and distribution of the company's profit. Develop a complete development strategy to establish the company's brand and attract more loans and investments. And sign agreements with venture capitalists to protect the company's legal rights and interests.