The Position of Our Platform in the Industrial Chain
The core of our project is a time-saving development platform for conducting proof-of-concept in the lab. Therefore, we are downstream of product selection and upstream of process scale-up in the entire biomanufacturing chain. What we need are a target product and the associated metabolic pathway analysis, and the chassis modification strategy determined from this information will serve as input to our platform.
Our platform is consistent with three parts: 1. Golden gate collection for cross-kingdom strain test, by which we can not only choose and demenstrate the production of the best strain, but also construct different strains so as to apply for more patents. 2. The platform for high level production of FPP, which will contribute the downstream production of value terpenes. 3. Vibrio natriegens and seawater fermentation, which will save fresh water resource and could produce rapidly.Our Target User
We are now building a company and we will use our platform to provide various of synbio products. Others will not be directly using our project, but this project provides us advantages to survive in the industry since we are capable to fit the various, fast-changing market need.
After success, this platform will be opned to those who wants to develope their strain rapidly, they may be synbio companies focusing on biomanufacturing or researchers focusing on strain engineering or bacsic science. In these cases, while this platform is our core competence, we will also authorize and cooperate with others for the boost of synthetic biology research and market in China.
How Do We Envision Others Using Our Project?
Our platform is consistent with three parts:
1. Golden gate collection for cross-kingdom strain test, by which we can not only choose and demenstrate the production of the best strain, but also construct different strains so as to apply for more patents. 2. The platform for high level production of FPP, which will contribute the downstream production of value terpenes. 3. Vibrio natriegens and seawater fermentation, which will save fresh water resource and could produce rapidly.According these, we will first unpload our golden gate collection into Addgene, so as to recommand and share and our parts with others. Next, the details of vibrio natriegens's seawater fermentation process will be open source, because this is a economical and energy efficient production strategy, we believed that many Synbio companies and researchers will paid attention to and pick it. AS for the FPP high level produtcion, the data of our research showed that it is amazing efficient, and Synbio companies and researchers will pay attention it gradually.
About Safety
Our project will be used in lab, as a replacement of traditional develop pipeline. It will not cause more harm than traditional ways.
Example
Here, we take our first product, (-)-α-bisabolol as an example.
(-)-α-bisabolol is a terpenoid whose production is dependent on the production of FPP. Therefore, we first identified the FPP production pathway to be introduced in our target chassis. Subsequently, we identified CcBOS as the downstream enzyme for the production of (-)-α-bisabolol.
Subsequently, we tested the production efficiency of the relevant pathways in E. coli and V. nat using our Golden Gate Collection for design. After obtaining the corresponding data, we completed the whole basic process of development. See results for details of (-)-α-bisabolol production.
For detailed technologies applied in this process, see our project design.
Further
Our project concerned on POC in lab, scale-up is still a problem that is beyond our project. However, with massive products we can develop in a short time, the risk of scale-up failure is considered acceptable.
For more information about our project application in real world, see our entrepreneurship page.