1. BP swot and final edition
2. edit wechat officail account
3. making promotion video
4. contact kindergarten education
5. franchising
6. write wiki franchasing
7. final presentation
8. design advertising leaflets
9. design team cartoon products
10. bp collaboration

1. Design team name and logo
2. Design product peripherals
3. Make Education Video and Promotion Video
4. Contact the community university and determine the preaching venue
5. Purchase peripheral products
6. Write an interview outline
7. Interview expert host
8. Write Wiki interview part and BP's 4P part
9. Make final PPT

1. BP operation management
2. Education ppt for kindergarten
3. collaboration 1 (team progress)
4. interviewing experts
5. Franchising
6. write Wiki
7. final presentation
8. present the education ppt
9. collaboration 2(team progres)
10. advertise

1. Participate in the design of team name and logo, and write team introduction
2. design questionnaires and collect data
3. Collaborate with other iGEM teams
4. Write Wiki collaboration part and Business plan part
5. participate in offline popularization
6. interview experts
7. Collaborate with wet team to promote our project

1. promote the questionnaire to obtain more effective data to support our project
2. Design expert interview questions
3. Interview experts
4. Collaborate with other iGEM teams

1. BP operational management and target consumer part, refine entering barriers part
2. Design team peripheral products
3. organize wet lab's interview questions
4. spread out questionaire
5. host of collaboration1 and made the powerpoint
6. draw education animation
7. dubbing actor of promotion video and education video
8. collaboration2 presenting
9. Final presentation powerpoint making

1. BP executive summary, compnay offerings, target customers and key competitors
2. Education ppt for community college
3. write interview questions
4. design, analyse and spread qustionnaire
5. present education ppt
6. write Wiki quetionnaire and education
7. BP collaboration
8. final presentation

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.
13.Help the dry team to do science popularization

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1. WIKI content
2. Help the dry team to do expert interview and collaborate with other iGEM teams
3. Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

1.Preparation of LB medium
2.PCR & Digestion of PCR Products
3.Plasmid Extraction
4.agarose gel electrophoresis
5.gel extraction
6.Preparation of competent cells
7.Double Digests
8.Assembly and transformation of recombinant plasmid
9.Construction of SbPETase mutagenic plasmids"
10.Purified protein
11.WIKI content
12.Write journals and summaries about daily experimental contents, principles, and steps of experiment.

❖ Medical Experimental Center， China Academy of Chinese Medical Sciences

❖ Beijing International Club
❖ Room e, floor 9, tower C, Oriental Ginza

❖ Project instructor: Prof. Liujie Huo. He has been participating in our project ideation, especially experiment design.
❖ Wet Lab: Dr.Riuifang Nie and Dr. Boya Nie
❖ Dry Lab: Ms.Ke Xu
❖ Business Plan Consultant: Mr, Winston Liu，ex- TMT industry player, business consultant, auditor, certified financial controller

❖ Dr. Zhenggang Han,University of Lubeck, Germany
❖ Dr. Yuxuan Chen, School of Environment and Resources, Zhejiang University
❖ Dr. Shiyuan Li,Director of Synthetic Biology Line in Cathy Fortune Capital Investment,PhD, Key Laboratory of Synthetic Biology, Chinese Academy of Sciences