Helicobacter pylori (H. pylori) is a spiral, gram-negative bacterium. Recently identified as a gastric carcinogen, its infection requires rapid and accurate detection methods; however, current mechanisms either lack in conveniency due to their reliance on complex equipment, or in sensitivity due to their tendency to produce false positive results. In our project, we developed an inexpensive, non-invasive, and reliable screening method for H. pylori that can be implemented in both domestic households and hospital settings. Using E. coli as a vector, we successfully synthesized oligo DNA segments to simulate H. pylori. We then designed a CRISPR Cas12a-sgRNA complex to identify and bind to our target DNA sequences, which would result in the non-specific cleavage of our ssDNA fluorescent reporter. To assess its performance, we conducted gel electrophoresis and fluorescence tests. Experimental evidence suggests that our diagnostic test expressed high sensitivity, selectivity, and rapidity.
