Parts

Overview

    This year, among the 184 parts designed, 92 of them are basic parts and the rest are composite parts. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website.

Basic Parts

    Among the 92 basic parts designed this year, BBa_K4195029 is our favorite one, the gene enables the OMVs and engineering bacteria to display the receptor, rLvAPN1, to neutralize toxins secreted by V. parahaemolyticus. Besides, many other basic parts have also been designed to construct our project.

Composite Parts

    We have designed a series of composite parts this year, and most of them could be useful tools for our design. And our favorite composite part is BBa_K4195130, which contains the sequence of ClyA-rLvAPN1 to display the receptor.

Part Collection

    This year, our team designed Part Collections related to OMVs surface display system and ribozyme splicing system. The surface display system includes two kinds of anchor proteins: ClyA and INPNC, and the ribozyme splicing system consists of three types of reporter proteins: β-lactamase, Luciferase and GFP.
    Surface display systems are often used in displaying functional proteins on the surface of bacteria. We further utilize our Part Collection to produce OMVs with surface displayed proteins. For ribozyme splicing system, different guide sequences and ribozyme splicing sites are designed to determine the most efficient combinations. Gladly, some parts in our Part Collection show outstanding results in detecting the input sequence of toxin proteins, both in vivo and in vitro.
    We’ve detailed our design and recorded all the experiment data to the registry. Surely these can be instructive for the design and characterization of many teams.