All of the parts created and documented over the course of the project are on this page. Below is a table of parts added to the registry. All parts seen here have been engineered by the Virginia iGEM Team using components of publicly-available antibody sequences.
We added a total of ten basic parts (BBa_K4477001-BBa_K4477010) and four composite parts (BBa_K4477011-BBa_K4477014) to the registry.
| Part Number | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4477001 | Coding, Anti-oxLDL IgG Light Chain | This sequence codes for the light chain of an anti-apoB(MDA) immunoglobulin G (IgG), an Orticumab-biosimilar antibody | Miranda Khoury | 654 bp |
| BBa_K4477002 | Coding, Anti-oxLDL IgG Heavy Chain | This sequence codes for the heavy chain of an anti-apoB(MDA) immunoglobulin G (IgG), an Orticumab-biosimilar antibody | Miranda Khoury | 1338 bp |
| BBa_K4477003 | Coding, IK17 Anti-oxLDL scFv Antibody | This sequence codes for a short chain variable fragment (scFv) derived from the anti-oxLDL IK17 antibody. Specifically, this scFv targets a protein structure called MDA-modified apo-B (apoB(MDA)) that is part of an oxLDL molecule. | Miranda Khoury | 795 bp |
| BBa_K4477004 | Coding, McPC603 Anti-oxLDL scFv Antibody | This sequence codes for a short chain variable fragment (scFv) derived from the anti-oxLDL McPC603 antibody, which targets oxidized phosphocholine on oxLDL. | Miranda Khoury | 798 bp |
| BBa_K4477005 | Coding, Anti-mouse Camelid VHH Antibody | This sequence codes for a VHH region antibody fragment, also referred to as an nanobody. It binds to the constant chain region of mouse-derived IgGs. | Miranda Khoury | 408 bp |
| BBa_K4477006 | RBS, Orticumab-Biosimilar Light Chain expression | RBS that Team Virginia 2022 designed with the Salis RBS calculator for 75% relative expression of an IgG light chain for an anti-oxLDL antibody we designed (BBa_K4477001). | Miranda Khoury | 27 bp |
| BBa_K4477007 | RBS, Orticumab-Biosimilar Heavy Chain expression | RBS that Team Virginia 2022 designed with the Salis RBS calculator for 75% relative expression of the heavy chain of our Orticumab biosimilar (anti-oxLDL) antibody (BBa_K4477002). | Miranda Khoury | 26 bp |
| BBa_K4477008 | RBS, IK17 scFv expression | RBS that Team Virginia 2022 designed with the Salis RBS calculator for 75% relative expression of one of our designed IgG heavy chains (BBa_K4477003). | Miranda Khoury | 30 bp |
| BBa_K4477009 | RBS, McPC603 scFv expression | RBS that Team Virginia 2022 designed with the Salis RBS calculator for 75% relative expression of one of our designed scFvs (BBa_K4477004). | Miranda Khoury | 31 bp |
| BBa_K4477010 | RBS, VHH expression | RBS that Team Virginia 2022 designed with the Salis RBS calculator for 75% relative expression of one of our designed VHH antibody fragments (BBa_K4477005). | Miranda Khoury | 39 bp |
| Part Number | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4477011 | Composite, Orticumab-biosimilar Anti-human oxLDL Complete Expression Cassette | This sequence codes for a full IgG anti-apoB(MDA). It is derived partially from Orticumab, a human antibody that is currently in development. | Miranda Khoury | 2186 bp |
| BBa_K4477012 | Composite, IK17 scFv Anti-oxLDL Complete Expression Cassette | TThis sequence codes for a short chain variable fragment (scFv) derived from the anti-oxLDL IK17 antibody, which binds to MDA-modified apolipoprotein B on oxLDL. | Miranda Khoury | 988 bp |
| BBa_K4477013 | Composite, McPC603 scFv Anti-oxLDL Complete Expression Cassette | This sequence codes for a short chain variable fragment (scFv) derived from the anti-phosphocholine McPC603 antibody, which binds to oxidized phosphocholine on oxLDL. | Miranda Khoury | 992 bp |
| BBa_K4477014 | Composite, Anti-mouse Camelid VHH Antibody Complete Expression Cassete | This sequence codes for a heavy chain variable fragment (VHH), also known as a nanobody, that binds to the Fc region of murine IgGs. | Miranda Khoury | 610 bp |