Parts

All the parts that have been used in this project are listed in the two following tables. Two parts are compatible with the standards of the iGEM competition. These parts are tal-fjo BBa_K4209000 , and cysP BBa K4209004.

Basic Parts

Registry code Part name Type Description State Compatibility Size
BBa_K4209000 tal-fjo Coding Conversion of tyrosine into coumaric acid New RFC 21 and 1000 1.5 kb
BBa K4209001 SULT1A1 Coding Conversion of coumaric acid into zosteric acid New - 876 bp
BBa K4209002 cysDNCQ Coding Coding Sulphate transporter complex New - 3.7kb
BBa K2194003 cysPUWA Coding Sulphate uptake protein Existing - 3.8kb
BBa K4209004 cysP Coding Coding Sulphate transporter complex New RFC 21 and 1000 1kb
BBa_K4209005 fitD Coding Transmembrane toxin New - 9kb
BBa K4209006 fitG Coding Activator of fitD toxin New - 981bp
BBa K3482011 Ptac promoter Promoter Inducible promoter for composite part containing SULT1A1 and tal-fjo existing - 55bp
BBa_M50032 T7 terminator Terminator Used for composite part containing SULT1A1, tal-fjo, cysPUWA, cysP, cysDNCQ Existing - 48bp
BBa_K4209017 RBS RBS Used for composite part containing SULT1A1, tal-fjo, cysPUWA, cysP, cysDNCQ New RFC 6bp
BBa_K3633015 T7 promoter Promoter Used for composite part containing cysPUWA, cysP, cysDNCQ Existing - 19bp
BBa K4209015 RBS RBS Used for composite part containing fitD and fitG New RFC 9bp
BBa_K4291000 Trc promoter Promoter Inducible promoter for composite parts containing fitD or fitG Existing - 30bp
BBa_K4278705 EM7 promoter Promoter Constitutive promoter for composite parts containing fitD or fitG Existing - 48bp
BBa_K4289010 Lambda t0 terminator Terminator Used for composite parts containing fitD or fitG Existing - 95bp

Composite part

Registry code Part name Type Description State Compatibility Size
BBa_K4209007 SULT1A1-tal-fjo Composite Part Catalytic enzymes New - 2.7kb
BBa_K4209008 tal-fjo-SULT1A1 Composite Part Catalytic enzymes New - 2.7kb
BBa_K4209009 cysP-cysDNCQ Composite Part Sulphate transporters New - 5kb
BBa_K4209010 cysPUWA-cysDNCQ Composite Part Sulphate transporters New - 7.8kb
BBa K4209011 fitD 234 Composite Part Inducible FitD toxin gene New - 10.6kb
BBa_K4209012 fitG 234 Composite Part Inducible FitD toxin activator gene New - 1.1kb
BBa_K4209013 fitD 2313 Composite Part ConstitutiveFitD toxin gene New - 9.2kb
BBa K4209014 fitG 2313 Composite Part ConstitutiveFitD toxin activator gene New - 1.1kb