Marta Marangoni, Zosteric Acid : Marta is one of our team captains. With her personality and dedication she lead, together with Alison, the team throughout the summer. She helped with the conceptualization of the subproject about Zosteric Acid as well as with the design of the primers and plasmids needed. In addition, she worked in the laboratory all the time leading the zosteric acid subproject, checked and provided laboratory supplies too. She presented our project at the Synbio conference in Zurich! She was also very involved with the Human Practices, by actively participating to meetings with experts and being involved in multiple interviews.
Alison Gandelin, Zosteric Acid, FitD : Alison is the team captain alongside Marta. She worked on the project promotion video with all her soul and dedication. Alison was a strong presence who would always ensure to keep everything on track. She worked on the conceptualization of the subproject about Zosteric Acid. She also worked on FitD, mainly on laboratory experiments and mussels treatment, and minorly on the wiki design. She hosted a podcast on invasive species on radio, at the “Fréquence Banane” with Mehdi. Additionally, she is the team contact with UManitoba regarding our partnership.
Emi Pedrazzi, FitD, Human Practices : Emi took to heart the idea of communicating the global problem of invasive species to a global audience. To do so, she created a whole book for children to help them understand the problem of invasive species. The book contains chapters about our team and our project, but also definitions, riddles, crosswords, and descriptions of invasive species in Switzerland. The book is translated into English, Italian, and French. She also translated a questionnaire into Italian for another iGEM team. Emi spent time in the laboratory and collected data during our experiments. She helped with the general organization and performed PCRs, and electrophoresis gels for the FitD project. She also communicated a lot with our assistants too to obtain relevant information and guidance when needed by the team. At the end of the summer, she cleaned the whole laboratory with Salomé. A true swiss-knife!
Filippo Rey, Zosteric Acid : Filippo is one of our laboratory managers. He ensured that everything went smoothly in the laboratory. He is also a Quagga provider and often went to the lake to get some mussels for our experiments. Also, he worked on the needs of the mussels and designed some ways to keep them alive long enough for the experiments. In the first part of the project he helped design the primers for the Zosteric Acid subproject and in the last weeks of the project he also assisted the group responsible for FitD toxin during the testing on mussels.
Douhan Wicht, Zosteric Acid, Wiki : He is a part of the subproject about Zosteric Acid and designed all of the primers for this part. During the summer, he first worked in the laboratory to build the plasmids for the production of zosteric acid and figured out what media was best for each of our strains of bacteria by creating growth curve plots in R. In the second part of the summer, apart from helping in the process of measuring how much zosteric acid our bacteria were producing, he mainly dedicated himself to the creation of the wiki and later the proofreading of all the wiki pages.
Albane Schmid, FitD : One of our laboratory managers, she spent all of her summer in the laboratory, working on the Fit D toxin part. She did a lot of cloning experimentation and also helped with the mussel tests. In the first steps of the project, she helped with the conceptualization and the background research. She also contributed to the design of our plasmids and primers. Furthermore, she translated our project promotion video into German and helped present our project at the ISME conference.
Jérémy Berger, FitD : He, too, passed all of his summertime in the laboratory. He is one of our lab managers and often stayed late to finish experiments with the Fit D toxin. In the early days, he worked on the conceptualization of the project and engineered some plasmids for FitD. He was responsible for the data curation, especially in the production of the Fit D toxin. He also often devoted himself to harvesting mussels in the lake.
Mehdi Naiah, FitD, DJ : Mehdi is our treasurer, so he always made sure that we got our money back. He worked on the FitD project from beginning to end and took over some experiments for the ZA even though he wasn’t originally a part of this project. He helped Emi with the kids’ book : he wrote some species sheets and also the page about DNA and genome. He wrote and presented the podcast at “Fréquence Banane” with Alison. In addition, he participated in the blogs’ collaboration with Chalmers-Gothenburg by doing an article about insulin. Mehdi also helped with the primers and plasmids design. Moreover, he often devoted himself to getting quaggas in the lake for our experiments. Finally, he also participated in the ISME18 presentation of our iGEM project. He is also our DJ and always blasted music in the laboratory to cheer us up!
Rosalie Marguerat, Human Practices, Blog : Rosalie helped the team to communicate with the rest of the world by being on time with social media posts and outreach activities. She wrote and posted on our blog, „The transcriptome“, with Chalmers-Gothenburg’s team. She often went by the lake to retrieve Quaggas for the laboratory. She managed the collaboration part on behalf of our team and ensured smooth communications with other teams. She participated in the elaboration of the species portraits for the collaboration with “La maison de la rivière”. She also took care of our team's equipment.
Salomé Ifergan, Human Practices : Salomé is also our communication officer. Together with Rosalie, she connected the team with social networks. Salomé is responsible for promoting blog articles on Instagram. She also worked on the promotional video along with Alison. Salomé also helped at the beginning of the summer with the anti-fouling project and with the kid's book at the end of the summer, when she also took part in the wiki and helped to design the pages for a better look!
Cindy Ramel, Human Practices, Quagga : Cindy is an outreach officer. She worked in collaboration with « La maison de la Rivière » for the exposition. Also, she helped with the writing of the children’s book with Emi, correcting it. She contacted teachers to promote it. She drew the first draft of the logo. Cindy also studied the various conditions necessary to keep the mussels alive in the lab and to observe their attachment to surfaces. She wrote all the main protocols needed for the lab.
Julie Tschanz, Human Practices, Wiki : Julie is involved in establishing human practice projects and as one of our webmasters. She first worked on the project's conceptualization with the human practice part, deciding who to call and what to do to make our project known. She then dedicated herself to the design of the logo. During the second part of the summer, she mainly worked on the wiki writing and styling.
Adrien Burki, FitD : Adrien was our most dedicated lab worker. He designed our plasmids and primers for the FitD project with Jérémy. He participated in the cloning of the different plasmids into E. coli and then into P. protegens. He did many PCRs, clean-ups, minipreps, nanodrops, and so on ! Our most important lab force !
Leïla Ouhamma, Human Practices, Secretary : She is our secretary and took care of the administrative part on our behalf. She organized our trip to Paris for the Jamboree. She also participated in the Human Practices team by contacting outside people needed to keep the project moving forward, and other people to help and advise us. She often participated in zoom meetings with other teams we collaborated with and ensured smooth communication with them, especially when organizing the SynBio conference. As she is keen on mathematics, she worked with Albane to develop a useful model for our project. She developed a slight talent for writing while contributing to the blog's perpetuation. She also joined the Wiki team at the end to fine-tune some final details. Working with mussels is not an easy task, therefore she worked on how to keep them alive from the very beginning and on the final concentration test experiments.
Primary PI
Yolanda Schaerli : Our teacher provided us with general and project support. She also helped us with our lab difficulties and was our overall project advisor support. She also coached us on the presentation and the parts of the human practice.
Instructors
Audam Chhun : Audam was responsible for our boot camp training and was one of our lab, technical support. He helped us a lot throughout the project by always being there and answering every question we had. He also was responsible for the safety of the laboratory.
Emanuele Boni : He supported us through the wiki by taking the time to try and understand it to help us. He also was big lab support and would always provide us with advice and materials when needed.
Astri Kusumawardhani: Astri was always there to give us great advice and to support us throughout the project. She corrected many pages for the wiki.
Sjoerd Seekles: Sjoerd was there from the beginning and helped us elaborate and conceptualize the project. He was also very present throughout the summer to help with the lab and corrected our pages for our wiki.
Roberto Avendaño: Roberto has a unique way of doing experiments. He helped us with ours and always found answers to our problems, such as finding a special machine for sonication, corrected our final versions of pages for our wiki.
Linh Ho: Linh Ho is the wiki advisor. She revised most of the pages and helped us to improve them accordingly. She was a great help, without her we wouldn’t have such a good wiki to present. We thank her a lot and are very glad for her help.
Project Advisors
Jordan Vacheron: Jordan Vacheron helped us a lot with the protocol for the Pseudomonas transformation. He supported us thoroughly during our project and gave us the wild-type strains for our experiments. We thank him deeply.
Michael Taschner: Michael Taschner helped tremendously in the making of the SDS page gel for our experiment. This allowed us to confirm our cloning part. We are very thankful for his help.
Frédéric Gumy: Frédéric Gumy ran the HPLC for our experiment on the Zosteric acid part. By doing so, we could measure the production of Zosteric Acid. We thank him deeply for his advice and his help for this part of our project.
Acknowledgments
Pierre Goloubinoff: Pr. Pierre Goloubinoff gave us advice concerning our SDS page gel for our fit D part. We thank him deeply.
Sara Mitri: We would like to thank Mrs. Mitri for her help and advice in our modeling.
Vincent de Bakker: We thank Vincent de Bakker for his help concerning the statistical analysis of our experiment.
Andrew Quinn: We thank Andrew Quinn, chemical engineer, for his help regarding the zosteric acid and cumeric acid quantification through CG-MS.
Nathalie Menetrey: Nathalie Menetrey is a specialist in the field of Quagga mussel. After getting a look at our project, Nathalie Menetrey helped us understand the Quagga mussel more. She gave us plenty of documents we lacked. We are very thankful for the time she managed to give to us.
Brigitte Schmidt and Eloisa Villalpando: Brigitte Schmid is responsible for the quagga problem in the city of Lausanne, a task which she carefully handed over to Mrs. Villalpando, who is now her successor. They both work at the water department. They gave us meaningful documentation on the quagga but also on the global situation of their invasion. We thank them deeply for the help provided.
La Maison de la Rivière: « La Maison de la Rivière » is an association dedicated to the communication and to the education of problematics around water ecosystem. They gave us a place to expose our project. Furthermore, they gave us indications of the current solutions used against the mussels and gave us ideas for an application of our product in real conditions. They suggested a coating for the pipes or even a solution of the Fit-D toxin to pour directly into the water.
UManitoba's team: We gladly thank the UManitoba iGEM’s team for their collaboration with us.
Gothenburg's team: We deeply thank the Gothenburg iGEM’s team for their collaboration with us on the blog « The proteasome ». We were very happy to write articles with their team.
Fréquence Banane: Fréquence banane is the live-radio from the university of Lausanne. They gave us the stage to talk about our project in a podcast and therefore allowed us to communicate our idea to all of the UNIL students. We thank them deeply for this opportunity.
L'Auditoire Unil: « L’Auditoire » is the UNIL journal. They interviewed Marta and published an article on our project, allowing us to reach more people in our university.
ISME: ISME is the International Symposium on Microbial Ecology that was held in Lausanne from the 14th of August to the 19th. They allowed us to have a booth at their exposition. We could discuss our project with people from all over the world. We gladly thank them for such an opportunity.
YouthScience: YouthScience is a collaboration aiming at the vulgarization of scientific subjects. They kindly posted an article about our project on their blog, allowing us to reach even more people. We gladly thank YouthScience for the opportunity.
"Comment ça marche": Jérémy contacted the vulgarization journal « comment ça marche ? » and they were very interested in our project. We thank them deeply for the article they published about our project and our team.
Alexander Schanne: : Before the summer, Alexander contacted us to participate in the Synbio conference organized in Zurich. Thanks to him, we were able to meet other teams and share our projects.
Joachim Ifergan: Joachim Ifergan is the brother of Salomé. He works as an informatician and helped us to design the CSS code for our wiki template. We are very thankful to him. Without him, we wouldn’t have such a good layout.
Sponsors
Promega
New England Biolabs
Integrated DNA Technologies, Inc
Microsynth AG
Twist Bioscience
SnapGene
Mora Mora
University of Lausanne, Faculty of Biology and Medicine