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CsgA Knockout

Transformation of PKD46 into MC4100 and MC4100-Z1

LC99 + pKD46
LC14 + pKD46
LC14 + pKD46
LC99 + pKD46

pkD3 PCR 8/1/22

Gel Results

lane 1: ladder, lane 2: pdk3 (control), lane 3/4: PCR amplicons

pKD3 PCR 8/3/22

Black
White
Black 2
White 2

lane 1: ladder, lane 2: pdk3 (control), lane 3/4: PCR amplicons

pkD3 PCR 8/4/22

pKD3-PCR-8_4

pkD3 8/4/22 trial 2

PCR Cleanup Protocol

pkD3 Transformation

8/4/22 transformation results

Transformation Results

pkD3 PCR 8/5/22

pKD3 PCR

pkD3 transformation 8/5/22

8/5 Transformation

pkD3 with DMSO 8/8/22

pkD3 with DMSO

pkD3 transformation 8/8/22

Transformation

pkD3 PCR with DMSA 8/9/22

PCR - pKD3 with DMSO 8/9

pkD3 PCR w/ new primers 8/10/22

pKD3 PCR with new primers

pkD3 PCR with old primers 8/10/22

pKD3 PCR 8/10

pKD3 transformation 8/11/2022

LB + cm25 LC14
LB + cm25 LC99
LB LC14
LB LC99

Successful transformation of LC14 and LC99 9/22/2022

Transformation of LC14 and LC99
LB LC99 + pkD46 and LC LC14 + pKD46

Colony PCR 10/5/22

Colony PCR

Lanes:

DNA ladder

MC4100 colony - negative control

MC4100 csgA KO colony

MC4100Z1 csgA KO colony

MG1655 csgA KO colony

MC4100 ackA KO colony

Successfully transformed pkD3 10/5/22

Transformations

The collaborators

Thanks for the collaborator teams and the sponsor of our university

UIUC Wisconsin Lutheran College CABBI

Contact

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