| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4436017 | Primer | Forward Primer for Lambda Red csgA knockout | Vedanta Kompella | 60 bp |
| BBa_K4436666 | Primer | Reverse Primer for Lambda Red csgA knockout | Vedanta Kompella | 60 bp |
| BBa_K4436690 | Promoter | narG CC(-40.5) promoter | Vedanta Kompella | 232 bp |
| Plasmid | Squence | Color Key |
|---|---|---|
| csgA_SpyTag_GFP w/ native promoter | aaatcataaaaaatttatctgctttgtgagcggataacaattataat agattcaattgtgagcggataacaatttcacactactagagaaggag gaattatactagatgaaacttttaaaagtagcagcaattgcagcaat cgtattctccggtagcgctctggcaggtgttgttcctcagtacggcg gcggcggtaaccacggtggtggcggtaataatagcggcccaaattct gagctgaacatttaccagtacggtggcggtaactctgcacttgctct gcaaactgatgcccgtaactctgacttgactattacccagcatggcg gcggtaatggtgcagatgttggtcagggctcagatgacagctcaatc gatctgacccaacgtggcttcggtaacagcgctactcttgatcagtg gaacggcaaaaattctgaaatgacggttaaacagttcggtggtggca acggtgctgcagttgaccagactgcatctaactcctccgtcaacgtg actcaggttggctttggtaacaacgcgaccgctcatcagtactacta gagggctcaggcggcagcggtgcacatattgtcatggtagatgcgta caaaccaaccaaatactagagaaggaggaattatactagactagacg taaaggagaagaacttttcactggagttgtcccaattcttgttgaat tagatggtgatgttaatgggcacaaattttctgtcagtggagagggt gaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac tactggaaaactacctgttccatggccaacacttgtcactactttcg gttatggtgttcaatgctttgcgagatacccagatcatatgaaacag catgactttttcaagagtgccatgcccgaaggttatgtacaggaaag aactatatttttcaaagatgacgggaactacaagacacgtgctgaag tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt attgattttaaagaagatggaaacattcttggacacaaattggaata caactataactcacacaatgtatacatcatggcagacaaacaaaaga atggaatcaaagttaacttcaaaattagacacaacattgaagatgga agcgttcaactagcagaccattatcaacaaaatactccaattggcga tggccctgtccttttaccagacaaccattacctgtccacacaatctg ccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt gagtttgtaacagctgctgggattacacatggcatggatgaactata caaacataatccggcaaaaaaacgggcaaggtgtcaccaccctgccc tttttctttaaaaccgaaaagattacttcgcgttattagcagaaagt caaaagcctccgaccggaggcttttgactaaaacttcccttggggtt atcattggg |
Red = T5 promoter Orange = T5 RBS Yellow = csgA Blue = GS linker Cyan = SpyTag Green = GFP Dark Blue = Terminator #1: His operon terminator Gray = Terminator #2 |
| csgA_SpyTag_GFP w/ sensitive promoter | ccattaatatgttacccatggggaatactccttaatacccatctgca taaaaatcttaatagtttaaataactacaggtataaaacgtcttaat ttacagtctgttatgtggtggctgttaattatcctaaaggggtatct taggaatttactttatttttcatccccatctaaatgtgatgtacatc acatggatccccctcactcctgccataattctgatatactagagaag gaggaattatactagatgaaacttttaaaagtagcagcaattgcagc aatcgtattctccggtagcgctctggcaggtgttgttcctcagtacg gcggcggcggtaaccacggtggtggcggtaataatagcggcccaaat tctgagctgaacatttaccagtacggtggcggtaactctgcacttgc tctgcaaactgatgcccgtaactctgacttgactattacccagcatg gcggcggtaatggtgcagatgttggtcagggctcagatgacagctca atcgatctgacccaacgtggcttcggtaacagcgctactcttgatca gtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtg gcaacggtgctgcagttgaccagactgcatctaactcctccgtcaac gtgactcaggttggctttggtaacaacgcgaccgctcatcagtacta ctagagggctcaggcggcagcggtgcacatattgtcatggtagatgc gtacaaaccaaccaaatactagagaaggaggaattatactagactag acgtaaaggagaagaacttttcactggagttgtcccaattcttgttg aattagatggtgatgttaatgggcacaaattttctgtcagtggagag ggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg cactactggaaaactacctgttccatggccaacacttgtcactactt tcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa cagcatgactttttcaagagtgccatgcccgaaggttatgtacagga aagaactatatttttcaaagatgacgggaactacaagacacgtgctg aagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaa ggtattgattttaaagaagatggaaacattcttggacacaaattgga atacaactataactcacacaatgtatacatcatggcagacaaacaaa agaatggaatcaaagttaacttcaaaattagacacaacattgaagat ggaagcgttcaactagcagaccattatcaacaaaatactccaattgg cgatggccctgtccttttaccagacaaccattacctgtccacacaat ctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt cttgagtttgtaacagctgctgggattacacatggcatggatgaact atacaaacataatccggcaaaaaaacgggcaaggtgtcaccaccctg ccctttttctttaaaaccgaaaagattacttcgcgttattagcagaaagtcaaaagcctccgaccggaggct tttgactaaaacttcccttggggttatcattggg |
Red = narG CC promoter (nitrate-sensitive) Orange = T5 RBS Blue = GS linker Yellow = csgA Cyan = SpyTag Green = GFP Dark Blue = Terminator #1: His operon terminator Gray = Terminator #2 |
| SpyCatcher_GFP-His w/ native promoter | caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc agctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa cgcaattaatgtgagttagctcactcattaggcaccccaggctttac actttatgcttccggctcgtatgttgtgtggaattgtgagcggataa caatttcacacatactagagaaagaggagaaatactagatgtcttat tatcatcatcaccatcaccacgattatgacattcctaccaccgagaa cctctacttccaaggcgccatggtcgataccctttccggactgtcca gcgaacagggccaatctggcgatatgacgatcgaagaggactctgca acgcacattaaatttagcaagcgggatgaggatggtaaggaactggc gggtgcgacgatggaactgcgggactcgagtggtaagacaatttcga cttggatttctgatggtcaagtcaaagatttctacttatacccgggt aaatacacttttgttgaaaccgcagcgccggatggctatgaagtggc aacggcaattaccttcaccgttaacgagcagggtcaggttaccgtga atggaaaggcaaccaagggcgatgctcatatttactagggctcaggc ggcagcggtactagacgtaaaggagaagaacttttcactggagttgt cccaattcttgttgaattagatggtgatgttaatgggcacaaatttt ctgtcagtggagagggtgaaggtgatgcaacatacggaaaact tacccttaaatttatttgcactactggaaaactacctgttcca tggccaacacttgtcactactttcggttatggtgttcaatgct ttgcgagatacccagatcatatgaaacagcatgactttttcaa gagtgccatgcccgaaggttatgtacaggaaagaactatattt ttcaaagatgacgggaactacaagacacgtgctgaagtcaagt ttgaaggtgatacccttgttaatagaatcgagttaaaaggtat tgattttaaagaagatggaaacattcttggacacaaattggaa tacaactataactcacacaatgtatacatcatggcagacaaac aaaagaatggaatcaaagttaacttcaaaattagacacaacat tgaagatggaagcgttcaactagcagaccattatcaacaaaat actccaattggcgatggccctgtccttttaccagacaaccatt acctgtccacacaatctgccctttcgaaagatcccaacgaaaa gagagaccacatggtccttcttgagtttgtaacagctgctggg attacacatggcatggatgaactatacaaacacatcaccatcaccatcactaatcc ggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaa accgaaaagattacttcgcgttattagcagaaagtcaaaagcctccg accggaggcttttgactaaaacttcccttggggttatcattggg |
Red = lacI regulated promoter Orange = RBS Yellow = SpyCatcher Blue = GS linker Cyan = SpyTag Green = GFP Magenta = 6x His Tag Dark Blue = Terminator #1: His operon terminator Gray = Terminator #2 |
| SpyCatcher_nrtA-His w/ native promoter | caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc agctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa cgcaattaatgtgagttagctcactcattaggcaccccaggctttac actttatgcttccggctcgtatgttgtgtggaattgtgagcggataa caatttcacacatactagagaaagaggagaaatactagatgtcttat tatcatcatcaccatcaccacgattatgacattcctaccaccgagaa cctctacttccaaggcgccatggtcgataccctttccggactgtcca gcgaacagggccaatctggcgatatgacgatcgaagaggactctgca acgcacattaaatttagcaagcgggatgaggatggtaaggaactggc gggtgcgacgatggaactgcgggactcgagtggtaagacaatttcga cttggatttctgatggtcaagtcaaagatttctacttatacccgggt aaatacacttttgttgaaaccgcagcgccggatggctatgaagtggc aacggcaattaccttcaccgttaacgagcagggtcaggttaccgtga atggaaaggcaaccaagggcgatgctcatatttactagggctcaggc ggcagcggtatgtccaatttttcccggtccacccggcggaaatttat gtttaccgccggcgccgccgccattggcggcgtggtgttgcatggct gtacctcccccaccaccacctccaccggcaccggcaccggctcctcc accgatcaagccatttcccccttggtggaaggcgaaaatgcccccga agtgaccaccgccaaattgggctttattgccttgaccgatgccgccc ccttgattattgccaaagaaaaaggcttttatgccaaatatggcatg cccgatgtggaagtgttgaaacaagcctcctggggcaccacccggga taatttggtgttgggctccgcctccggcggcattgatggcgcccata ttttgacccccatgccctatttgattaccatgggcaccgtgaccgat ggcaaacccacccccatgtatattttggcccggttgaatgtgaatgg ccaaggcattcaattgggcaataattataaagatttgaaagtgggca ccgatgccgcccccttgaaagaagcctttgccaaagtgaccgatccc aaagtggccatgacctttcccggcggcacccatgatatgtggattcg gtattggttggccgccggcggcatggaacccggcaaagatttttcca ccattgtggtgccccccgcccaaatggtggccaatgtgaaagtgaat gccatggaatccttttgtgtgggcgaaccctggcccttgcaaaccgt gaatcaaggcgtgggctatcaagccttgaccaccggccaattgtgga aagatcatcccgaaaaagcctttggcatgcgggccgattgggtggat caaaatcccaaagccgccaaagccttgttgatggccgtgatggaagc ccaacaatggtgtgatcaagccgaaaataaagaagaaatgtgtcaaa ttttgtccaaacgggaatggtttaaagtgccctttgaagatattatt gatcggtccaaaggcatttataattttggcaatggccaagaaacctt tgaagatcaagaaattatgcaaaaatattgggtggataatgcctcct atccctataaatcccatgatcaatggtttttgaccgaaaatattcgg tggggctatttgcccgcctccaccgataccaaagccattgtggataa agtgaatcgggaagatttgtggcgggaagccgcccaagccttggaag tgcccgccgatcaaattccctcctccccctcccggggcattgaaacc ttttttgatggcattacctttgatcccgaaaatccccaagcctattt ggattccttgaaaattaaatccattaaagcccatcaccatcaccatc actagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttt tctttaaaaccgaaaagattacttcgcgttattagcagaaagtcaaa agcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg |
Red = lacI regulated promoter Orange = RBS Yellow = SpyCatcher Blue = GS linker Cyan = nrtA Magenta = 6x His Tag Dark Blue = Terminator #1: His operon terminator Gray = Terminator #2 |
| SpyCatcher_nrtA-His w/ sensitive promoter | ccattaatatgttacccatggggaatactccttaatacccatctgca taaaaatcttaatagtttaaataactacaggtataaaacgtcttaatt tacagtctgttatgtggtggctgttaattatcctaaaggggtatctta ggaatttactttatttttcatccccatctaaatgtgatgtacatcaca tggatccccctcactcctgccataattctgatatactagagaaa gaggagaaatactagatgtcttattatcatcatcaccatcaccacga ttatgacattcctaccaccgagaacctctacttccaaggcgccatgg tcgataccctttccggactgtccagcgaacagggccaatctgg cgatatgacgatcgaagaggactctgcaacgcacattaaattt agcaagcgggatgaggatggtaaggaactggcgggtgcgacga tggaactgcgggactcgagtggtaagacaatttcgacttggat ttctgatggtcaagtcaaagatttctacttatacccgggtaaa tacacttttgttgaaaccgcagcgccggatggctatgaagtgg caacggcaattaccttcaccgttaacgagcagggtcaggttac cgtgaatggaaaggcaaccaagggcgatgctcatatttactagggctcaggcggcagcggtatgtccaatttttc ccggtccacccggcggaaatttatgtttaccgccggcgccgcc gccattggcggcgtggtgttgcatggctgtacctcccccacca ccacctccaccggcaccggcaccggctcctccaccgatcaagc catttcccccttggtggaaggcgaaaatgcccccgaagtgacc accgccaaattgggctttattgccttgaccgatgccgccccct tgattattgccaaagaaaaaggcttttatgccaaatatggcat gcccgatgtggaagtgttgaaacaagcctcctggggcaccacc cgggataatttggtgttgggctccgcctccggcggcattgatg gcgcccatattttgacccccatgccctatttgattaccatggg caccgtgaccgatggcaaacccacccccatgtatattttggcc cggttgaatgtgaatggccaaggcattcaattgggcaataatt ataaagatttgaaagtgggcaccgatgccgcccccttgaaaga agcctttgccaaagtgaccgatcccaaagtggccatgaccttt cccggcggcacccatgatatgtggattcggtattggttggccg ccggcggcatggaacccggcaaagatttttccaccattgtggt gccccccgcccaaatggtggccaatgtgaaagtgaatgccatg gaatccttttgtgtgggcgaaccctggcccttgcaaaccgtga atcaaggcgtgggctatcaagccttgaccaccggccaattgtg gaaagatcatcccgaaaaagcctttggcatgcgggccgattgg gtggatcaaaatcccaaagccgccaaagccttgttgatggccg tgatggaagcccaacaatggtgtgatcaagccgaaaataaaga agaaatgtgtcaaattttgtccaaacgggaatggtttaaagtg ccctttgaagatattattgatcggtccaaaggcatttataatt ttggcaatggccaagaaacctttgaagatcaagaaattatgca aaaatattgggtggataatgcctcctatccctataaatcccat gatcaatggtttttgaccgaaaatattcggtggggctatttgc ccgcctccaccgataccaaagccattgtggataaagtgaatcg ggaagatttgtggcgggaagccgcccaagccttggaagtgccc gccgatcaaattccctcctccccctcccggggcattgaaacct tttttgatggcattacctttgatcccgaaaatccccaagccta tttggattccttgaaaattaaatccattaaagcccatcaccatcaccatcactagtccggcaaaaaaacgggc aaggtgtcaccaccctgccctttttctttaaaaccgaaaagattact tcgcgttattagcagaaagtcaaaagcctccgaccggaggc ttttgactaaaacttcccttggggttatcattggg |
Red = narG CC promoter (nitrate-sensitive) Orange = RBS Yellow = SpyCatcher Blue = GS linker Cyan = nrtA Magenta = 6x His Tag Dark Blue = Terminator #1: His operon terminator Gray = Terminator #2 |
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