Code

Type

Name

Description

BBa_K4422017

Composite

Removable and Flexible 8X-His tag (8X His-tag / Short Flexible Linker Gly-Gly-Ser-Gly / Xa Factor Cleavage Site)

Flexible polyhistidine tag, which is flexible preventing steric hindrance. In case the tag has an effect in the protein, it can be removed after purification. This is an ideal tag for peptides.

BBa_K4422014

Composite

eGFP coexpressed

Allows the coexpression of eGFP due to a T2A cleaving peptide.

BBa_K4422020

Composite

Bioactive AcTx-Hv2a toxin (AcTx-Hv2a / 3X Ala Linker /Lectin)

Bioactive pesticide toxin for insect ingestion

BBa_K4422021

Composite

Bioactive OAIP-1 toxin (OAIP-1 / 3X Ala Linker /Lectin)

Bioactive pesticide toxin for insect ingestion

BBa_K4422022

Composite

Purification and Reporter Device (Promoter AOX1/ Kozak/ 8X His-tag / Short Flexible Linker Gly-Gly-Ser-Gly / Xa Factor Cleavage Site/ Restriction Enzyme KpnI / Restriction Enzyme XhoI / T2A / eGFP)

This part allows the purification and detection of recombinant proteins, by using the restriction enzymes KpnI and XhoI