Code

Type

Name

Description

Tag

Internal c-Myc (Pichia pastoris codon optimized)

Polypeptide tag, which can be fused to either the C- or N- terminus of a protein. It is used for protein quantification through western blot.

Coding

U1-theraphotoxin Sp1a / OAIP-1 (Pichia pastoris codon optimized)

34 amino acid peptide, which forms an inhibitory knot with insecticidal activity by interacting with target insects' ion channels.

Coding

GNA Lectin (Pichia pastoris codon optimized)

Peptide carrier used in insecticidal proteins to cross the insect gut after oral ingestion. It will carry the peptide toxins to the insects' hemolymph.

Other

T2A Self Cleaving Peptide (Pichia pastoris codon optimized)

Used for polycistronic expression by cleavage during translation in eukaryotic cells due to a ribosomal skip.

Other

Xa Factor Cleavage Site (Pichia pastoris codon optimized)

Cleaving site target of the Factor Xa Protease. Allows the processing of recombinant proteins such as the cleavage and removal of His-tags.

Protein Domain

Short Flexible Linker Gly-Gly-Ser-Gly (Pichia pastoris codon optimized)

Linker, which connects two parts, adding additional space in between. This linker allows the movement of the parts.

Coding

Omega-hexatoxin-Hv2a / AcTx-Hv2a (Pichia pastoris codon optimized)

45 amino acid peptide, which contains a target="_blank" hydrophobic C-terminal tail that interacts with insect ion channels. Thus, this peptide has insecticidal activity.

Tag

8X His-tag

Polyhistidine tag that can be placed on either the N or C terminus to purify recombinant proteins using affinity chromatography.

Protein Domain

3X Ala Linker (Pichia pastoris codon optimized)

Linker, which connects two parts, adding 3 alanines in between. This linker is flexible, allowing the movements of the parts.