Parts

The parts we used.

Overview

In the Anatoxic project we have added 3 new basic parts and 4 new composite parts to the registry.

The basic parts are:

Name Type Description Designers Length
BBa_K4208000 Ligand Binding Domain PctD-LBD 6xHisTag Ferran Agulló 1074 bp
BBa_K4208001 Hybrid Receptor PctD EnvZ HAMP Hybrid Ferran Agulló 1956 bp
BBa_K4208002 Hybrid Receptor PctD HAMP EnvZ Ferran Agulló 1992 bp

The composite parts are:

Name Type Description Designers Length
BBa_K4208003 PctD-LBD Expression Cassete Ferran Agulló 2354 bp
BBa_K4208004 PctD EnvZ HAMP Hybrid receptor inducible Expression with nahR promoter Ferran Agulló 3074 bp
BBa_K4208005 PctD HAMP EnvZ Hybrid receptor inducible Expression with nahR promoter Ferran Agulló 3110 bp
BBa_K4208006 OmpC GFP Inducible Expression Ferran Agulló 955 bp

From previous iGEM teams we have used some parts in our constructs including regulatory elements and a fluorescent protein:

Name Type Description Designers Length
BBa_K864600 Terminator T0 transcriptional terminator Erik Lundin, Team Upsala 2012 52 bp
BBa_J61048 Terminator T1 terminator John Anderson,Arkin Lab(2007) 113 bp
BBa_K228004 Regulatory NahR promoter Lin Min 1052 bp
BBa_C0012 Coding lacl lacI repressor Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton . . .
BBa_R0082 Regulatory OmpR OmpR promoter Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) . . .
BBa_E0040 Coding Coding GFP jcbraff . . .

For the assembly, the plasmids were kindly provided by Victor Sourjik’s lab from the Max Planck Institute of Terrestrial Microbiology in Marburg except for the Basic Parts (BBa_K4208001, BBa_K4208002) and Composite Parts (BBa_K4208004, BBa_K4208005) which were cloned via Gibson Assembly to create the hybrid receptors with the backbone pKG116 initially coding for the PctD-Tar Hybrid.