The parts we used.
In the Anatoxic project we have added 3 new basic parts and 4 new composite parts to the registry.
Name | Type | Description | Designers | Length |
---|---|---|---|---|
BBa_K4208000 | Ligand Binding Domain | PctD-LBD 6xHisTag | Ferran Agulló | 1074 bp |
BBa_K4208001 | Hybrid Receptor | PctD EnvZ HAMP Hybrid | Ferran Agulló | 1956 bp |
BBa_K4208002 | Hybrid Receptor | PctD HAMP EnvZ | Ferran Agulló | 1992 bp |
Name | Type | Description | Designers | Length |
---|---|---|---|---|
BBa_K4208003 | PctD-LBD | Expression Cassete | Ferran Agulló | 2354 bp |
BBa_K4208004 | PctD EnvZ HAMP | Hybrid receptor inducible Expression with nahR promoter | Ferran Agulló | 3074 bp |
BBa_K4208005 | PctD HAMP EnvZ | Hybrid receptor inducible Expression with nahR promoter | Ferran Agulló | 3110 bp |
BBa_K4208006 | OmpC GFP | Inducible Expression | Ferran Agulló | 955 bp |
Name | Type | Description | Designers | Length |
---|---|---|---|---|
BBa_K864600 | Terminator | T0 transcriptional terminator | Erik Lundin, Team Upsala 2012 | 52 bp |
BBa_J61048 | Terminator | T1 terminator | John Anderson,Arkin Lab(2007) | 113 bp |
BBa_K228004 | Regulatory | NahR promoter | Lin Min | 1052 bp |
BBa_C0012 | Coding lacl | lacI repressor | Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton | . . . |
BBa_R0082 | Regulatory OmpR | OmpR promoter | Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) | . . . |
BBa_E0040 | Coding | Coding GFP | jcbraff | . . . |
For the assembly, the plasmids were kindly provided by Victor Sourjik’s lab from the Max Planck Institute of Terrestrial Microbiology in Marburg except for the Basic Parts (BBa_K4208001, BBa_K4208002) and Composite Parts (BBa_K4208004, BBa_K4208005) which were cloned via Gibson Assembly to create the hybrid receptors with the backbone pKG116 initially coding for the PctD-Tar Hybrid.