Attributions | Tuebingen

Our contributions:

We organized our team into different sub-teams to divide all the tasks that needed to be carried out into different groups. Following, you can see the different sub-teams each team members worked in, as well as the major contributions to the project of each individual.

Team member	Sub-Teams	Major Contribution
Elizaveta Shcherbinina	Public relations, human practices, wetlab, video, design	Animation for our video Human practice organisation Carrying out wetlab experiments Creation of instagram posts Team fotographer
Louis Funk	Video, Finance	Script Video filming and cutting Sponsor acquisition
Susan Tober	Finance,Human Practices, Wetlab, Video	Organisation of all payments Writing text for public outreach Carrying out wetlab experiments
Jakob Franz	Website, Video, Wiki, Design, Drylab	Creation of our website Implementation of our wiki Script and cutting for our video
Justina Jüttner	Wetlab, Wiki	Organisation of wiki content Carrying out wetlab experiments Experimental design for wetlab
Ferran Agulló i Manobens	Wetlab, Finance	Experimental design for wetlab Carrying out wetlab experiments
Lars Kötter	Wetlab, Drylab, Human Practices	Experimental design for wetlab Experimental design for drylab Carrying out wetlab experiments Twitter communication/li>
Émile Barbé	Drylab	Experimental design for drylab Performing drylab experiments
Laurentien Jungkamp	Design, Wiki, Wetlab	Design of logo, carrying out wetlab experiments
Jiajie Xiang	Wetlab, Finance	Experimental design for wetlab Carrying out wetlab experiments Sponsor acquistions
Rafaela Gamboa von Groll	Wetlab, Human Practices	Human practices organisation Carrying out wetlab experiments
Erva Keskin	Wetlab, Public Relations	Creation of instagram posts Carrying out wetlab experiments experimental design for wet lab
Garvit Sharma	Drylab, Wetlab	Help with wetlab experiments and drylab experiments

External Contributions:

Of course, participating in the iGEM competetion is not possible without lots of assistence.
Big thank you to:

Principal Investigator:

Dr. Libera Po Presti

For having our back and helping us with all official forms and regulations we needed to navigate. For constant advice regarding the design of our project. And for connecting us to other researchers to help us.

Mentors:

Inga Leske
Aarón Refisch
Adrián Vojtaššák
Erik Zimmer

Advisors from previous year's team:

Elias Ball
Teresa Müller

Lab space and general support:

Prof. Dr. Karl Forchhammer and his whole group (University of Tuebingen):

For accomodating us into their lab at the Interfaculty Institute of Microbiology and Infection Medicine at the University of Tuebingen

Dr. Iris Maldener (University of Tuebingen)

For giving us safety advice and making sure we comply to all safety regulations, including the use of anatoxin-a.

Dr. Khaled Selim (University of Tuebingen)

For introducing us to the lab and helping us with questions regarding equipment.

Design of a hybrid chemoreceptor:

Prof. Dr. Victor Sourjik and Dr. Wenhao Xu (MPI Marburg, Department of Systems and Synthetic Microbiology)

For providing us with reporter plasmids for EnvZ-based chimeric receptors, the expression plasmids for PctD expression in E. coli, plasmids carrying parts of PctD needed for chimeric receptor generation, and the background strain devoid of EnvZ and chemoreceptors for our biosesensor.

Dr. Wenhao Xu specifially for advice on the cloning for our project, starting from the provided plasmids.

Without their help, the project would have been much harder to realize.

Prof. Dr. Tino Krell (Experimental del Zaidín Institute, Granada, Spain)

For general advice on the PctD (PA4633) chemoreceptor and for advice on the expression of the PctD-ligand binding domain.

Prof. em. Dr. Joachim E. Schultz (University of Tuebingen)

For advice on the design of the chimeric PctD-EnvZ chemoreceptor.

Wetlab support:

Dr. Filipp Oesterhelt (University of Tuebingen)

For showing us how to plan, carry out and analysis isothermal titration calorimetrie (ITC) experiments.

Tim Orthwein (University of Tuebingen)

For introducing us into the Äkta FPLC machine the Forchhammer lab uses and providing us with an HisTrap column to purify the PctD-LBD protein with.

Drylab Support:

Dr. Philipp Thiel (University of Tuebingen)

For advising us on the programs to use and help with interpreting dry lab results.

iGEM Team Patras

For collaborating with us to help us getting started with GROMACS and performing the molecular dynamic simulation with us.

Advice on initial project choice:

Prof. Dr. Karl Forchhammer (University of Tuebingen)

Dr. Sebastian Molitor (University of Tuebingen)

Prof. Dr. Hannes Link (University of Tuebingen)

Human practices and public outreach:

Dr. Jens Fleischer, LGA Stuttgart and Dr. Gerhard Thielert, CVUA Sigmaringen

For showing us around in their respective labs and giving us insight into the testing of water for microbiological or chemical contaminants, as well as advice on the requirements for our biosensor and how the biosensor could be incorporated into the testing routine.