Attributions

Attributions

Many of our team had different roles which contributed to our project. In our attributions, we try to break down this contribution as closely as possible.


As a team, we would like to thank all our supporters who have contributed in any way to our project. First of all, we want to thank our supervisor Professor Dr. Boas Pucker. He provided us with laboratory space, helped us with the organization of finances and supported us during our iGEM journey.

General support

We thank:
Prof. Dr. Andre Fleißner and Ulrike Brandt for the support in the procurement of enzymes
Ute Kyrath and Martina Rülicke-Tunier for administration support
Schaghaiegh Monzavi for her support in administering contracts
Stabsstelle Presse und Kommunikation from TU Braunschweig for reading and improving articles about iGEM which were published in the TU Magazine
Prof. Dr. Stefan Dübel who gave us valuable tips throughout our iGEM experience

Scientific support

We thank:
Prof. Dr. Thorsten Seidel for the advice about suitable FRET couples and the donation of a plasmid containing mTurquoise and eYFP for our Reporter component
Carsten Hain and Lars Wollenschläger for the donation of the mRFP Plasmid
Prof. Dr. Theo Lange and Dr. Maria Pimenta Lange for the initial provision of a -80°C freezer and the joint use of a UV table for the visualization of our agarose gels
Prof. Dr. Michael Hust for information and advice about the use of scFv-Fragments in our detection component
Prof. Dr. Karsten Müller for information and advice about the use of scFv-Fragments in our detection component
PD Dr. Rebekka Biedendieck for the provision of a sonification device in order to be able to perform an ultrasound digestion of our cells

A special thanks goes to Martin Johann who provided us with a 3D printer to be able to build our detection box. He also advised us on many aspects of how best to proceed with the hardware design (Introduction in AutoCAD Inventor)

Supporters

Here we thank our supporters for providing us with lab equipment as well as donations:

  • BioRender
  • Jena Bioscience
  • DWK Life Sciences
  • Integra
  • New England Biolabs
  • SnapGene
  • Infors HT
  • Eurofins Genomics
  • BRAND
  • Julabo
  • Oxford Nanopore Technologies
  • Denios
  • abcr Chemie
  • Promega
  • Eppendorf
  • Macherey Nagel
  • Thermo Fisher Scientific
  • KGW Isotherm
  • IKA
  • Starlab
  • IDT-Biologika
  • SIGMA Laborzentrifugen GmbH
  • Kötterman
  • Xylem analytics
  • Sigma Aldrich
  • Sarstedt


Other mentionable support

We also thank:
Michael Burgis for the support in the creation of our wiki and for the preparation and for the support in the preparation for our presentation which we will present at the Grand Jamboree
Melina Meckoni for editing and cutting our project promotion video
Christian Köcher for the issuance of donation receipts and the mediation of contacts
Katharina Wolf for the support in the preparation for our presentation which we will present at the Grand Jamboree
Gabriel Zmed for the creation of our wiki design
Ben Oetken and Christoph Klaus for playing the superhero and the doctor in our project promotion video
Maximilian Edich for help in modeling a 3D protein structure
Dr. Jörg Meckoni for answering our questions about esophageal cancer detection

Team attributions


Melina Nowak

Social Media

Instagram and Facebook

  • Creating several posts for our Instagram Account, including MemeMonday, TechniqueThursday and ScienceSaturday, posting of updates, reels and stories
  • Head behind the idea of iGEM spreads awareness
  • Participation in the brainstorming of the team logo
  • Planning, production and editing of Instagram takeover from TU Braunschweig account and Promega
Twitter
  • Responsible for the iGEM TU Braunschweig Twitter account

Collaboration

  • Meetings for collaborations with several iGEM Teams
    • Andreu from iGEM Barcelona 2020 (Hormonic), iGEM Wageningen, iGEM ASIJ, iGEM CUHK-Hong Kong-SBS Atreus, iGEM Thessaloniki
  • Translation of an Article (iGEM chalmers organized with Uni Lausanne)

Education

  • Participation in the creation of the post for #IchBinHanna and the open source document
  • Participation in the partnership with iGEM ASIJ_TOKYO (Includes the constant exchange, planning and meetings)
  • Participation in the development of the idea for cancer awareness campaign as well as Leadership of the cancer awareness campaign (Includes writing to iGEM teams to ask about participation; meetings with potential teams as well as creating posts and managing the Instagram account)

Excursion

  • IDT Biologika
  • junior jam in Münster and presented our project
  • trade fair in Hamburg (Labsupply)
  • Frankfurt (Achema 2022)

Meeting with sponsors

  • Meetings with companies e.g., Promega, Infors HT, abcr, Starlab for social media
  • Planning, production and editing of Instagram takeover and stories for companies (Promega, Starlab, abcr, Infors HT)

Wiki

  • Organizational management of Wiki structure/content
  • Creator of Pages: Collaborations, Partnership, Attributions, Proposed Implementation, Social Media and the Description of Inteins

Wet lab

  • Amplification of the gene synthesis (pNpuN and pNpuC)
  • Gel electrophoresis (control for NpuC caged)
  • Resuspension Template (pNpuN insert)
  • PCR (NpuC insert)
  • Gelelectrophoresis (control for NpuN)
  • Plasmid resuspination from iGEM Distribution Kit
  • Chemical transformation of E.coli DH5alpha
  • Pre-cultivation and Plasmid preparation
  • Several Restriction digest of Insert and Vector
  • Colony PCR
  • PCR FTEV long and short
  • Preparation NpuC for sequencing

Others

  • Administration of the website
  • Elaboration of esophageal cancer application for our project
  • Responsible for the judging form
  • Part of the project promotion video
    • Casting of actors as well as starring in the video
    • Responsible for the upload
    • Planning the content and implementation of the video
  • Participation in the introduction to high performance liquid chromatography (HPLC)
  • Editing of the text for the report sent to Eurofins Genomics
  • Design of the presentation and biological illustrations
  • Taking pictures of all collaboration meetings and visits
  • Acquisition of energy drinks for the Team

Benjamin Harder

Organization of the visit of politicians, lecture by an expert on pH-value and a meeting with two representatives of Macherey Nagel

Excursion

  • IDT Biologika
  • trade fair in Hamburg (Labsupply)
  • Frankfurt (Achema 2022)

Sponsor acquisition

  • BioRender
  • Jena Bioscience
  • DWK Life Sciences
  • Integra
  • New England Biolabs
  • SnapGene
  • Infors HT
  • Eurofins Genomics
  • BRAND
  • Julabo
  • Oxford Nanopore Technologies
  • Denios
  • abcr Chemie
  • Promega
  • Eppendorf
  • Macherey Nagel
  • Thermo Fisher Scientific
  • KGW Isotherm
  • IKA
  • Starlab
  • IDT-Biologika
  • SIGMA Laborzentrifugen GmbH
  • Kötterman
  • Xylem analytics
  • Sigma Aldrich
  • Sarstedt
  • Filming project video for crowd funding

Wiki

  • Creator of pages: Communication, Description, Notebook and Integrated Human Practices

Wet lab

  • Cloning of the amplification component with PCR, transformation and following Gibson Assembly
  • Selecting the clone with colony PCR with following sequencing
  • Expression and purification of proteins for the reporter component
  • Measuring the FRET signal with and without TEX protease
  • participated in the production of competent cells for the

Others

  • Application for travel grant written and successfully obtained
  • from iGEM Barcelona 2020 (Hormonic)
  • Invitation of politicians for information exchange and project promotion
  • writing and preparing sponsorship contracts, Managing legal documents in close collaboration with legal department
  • Organization a seminar about pH measurement
  • Organization in a lecture by an expert about PH value
  • Meeting with two representatives of MACHEREY-NAGEL

Fabienne Spang

Wiki
  • Creator of pages: Proof of Concept, Parts collection, Parts and Improve

Education
  • Held a lecture in an advanced biology course in a school

Others

  • Maintained and updated the website
  • Maintained contact with the press office from our university
  • Translation of an article (iGEM Chalmers organized with Uni Lausanne)
  • Wrote a text for the awareness campaign

Frauke Drobek

Collaboration

  • Meeting for collaborations with iGEM Teams
    • Andreu from iGEM Barcelona 2020 (Hormonic)

Wiki

  • Creator of pages: Contribution and Safety

Wet lab

  • Worked on the reporter component
  • Preparation of chemically competent cells
  • Plasmid preparation and transformation of E. coli
  • colony PCR
  • Protein expression

Others

  • Maintained and updated the website
  • Helped with the project promotion video
  • Wrote a project information text for eurofins

Samuel Nestor Meckoni

Excursion

  • IDT Biologika
  • junior jam in Münster and presented our project
  • trade fair in Hamburg (Labsupply)
  • Frankfurt (Achema 2022)

Wiki

  • Creator of pages: Modeling, a big part of description, general correction of wiki pages (formating and speeling)

Others

  • Main head behind our project idea
  • Design of the wetlab project
  • Checking of primers and plasmids
  • Discussions with experts Nadja Henke, Thorsten Seidel
  • Participation in the introduction to high performance liquid chromatography (HPLC)
  • Moderation of the team meetings
  • iGEM project safety form
  • Creation of our poster for junior jam Münster
  • ides, design and implementation of our model
  • supporting the planning process and implementation of our hardware
  • Preparation and speaker of a presentation we presented in front of our professors at TU Braunschweig
  • supporting the acquisition of donations
  • Part of the project promotion video
    • Planning the content and implementation of the video

Simon Klute

Wiki

  • Creator of pages: Experiments

Dry lab

  • Designing Sequences for Genesynthesis:
    • NTEV_NpuN_caged
    • CTEV_NpuC_caged
  • Designing plasmid construct in silico:
    • NTEV_NpuN_his_caged
    • CTEV_NpuC_his_caged
    • FRET
  • Designing of Primers in silico:
    • PCR Amplification Primers for NTEV_NpuN_caged
    • PCR Amplification Primers for NTEV_NpuC_caged
    • Primers for adding his-tag and NEbuilder:
      • NTEV_NpuN_caged
      • >NTEV_NpuC_caged
    • Universal Sequencing Primers for all Constructs

Wet lab

  • Plasmid Preparation of pSB1C3
  • Linearisation through PCR and Purufication of pSB1C3
  • Construction of NTEV_NpuN_caged and NTEV_NpuC_caged with NEBuilder
    • Here amplification of inserts
    • Purification of inserts
    • Analytic gelelectrophoresis after Transformation E. coli with constructs
  • Construction of Full_TEV
    • PCR amplification of fragments

Others

  • iGEM project safety form
  • writing an article for BIOspektrum

Sina Huxhagen

Social Media

Instagram and Facebook

  • Creating several posts for our Instagram Account, including MemeMonday, TechniqueThursday and ScienceSaturday, posting of updates, reels and stories
  • Head behind the idea of iGEM spreads awareness
  • Participation in the brainstorming of the team logo as well as creating it
  • Planning, production and editing of Instagram takeover from TU Braunschweig account and Promega
  • Participation in the creation of the individual team member introduction posts

Collaboration

  • Meetings for collaborations with several iGEM teams
    • Andreu from iGEM Barcelona 2020 (Hormonic), iGEM Wageningen, iGEM ASIJ, iGEM CUHK-Hong Kong-SBS Atreus, iGEM Thessaloniki

Education

  • Preparation of the school presentation and presentation of it in the school
  • Creation of the safety kit, Participation in the creation of the post for #IchBinHanna and the open source document
  • Participation in the partnership with iGEM ASIJ_TOKYO (Includes the constant exchange, planning and meetings)
  • Participation in the development of the idea for cancer awareness campaign as well as leadership of the cancer awareness campaign (Includes writing to iGEM teams to ask about participation; meetings with potential teams as well as creating posts and managing the Instagram account)

Excursion

  • IDT Biologika
  • junior jam in Münster and presented our project

Meeting with sponsors

  • Participation in the brainstorming and filming of the video for Promega, as well as editing of the video

Wiki

  • Organizational management of Wiki structure/content
  • Creator of pages: Human Practices + Education + Hardware + Engineering

Dry lab

  • Introducing the idea of working with Inteines in the amplification component as well as inform about the possibilities
  • Participation in the creation of the plasmids of the amplification component

Wet lab

  • Participation in the laboratory work of the amplification component

Hardware

  • Part of the project promotion video
    • Searchin g for actors, as well as taking on an acting role
    • Planning the Content and implementation of the video
    • Editing of the video
  • Participation in the introduction to high performance liquid chromatography (HPLC)
  • Editing of the text for the report sent to Eurofins Genomics
  • Design of the team clothing
  • Design of biological illustrations

Dorothee-Sophie Börstling

Social Media

Instagram and Facebook

  • Participation in the creation and posting of updates, reels and stories
  • Planning, production and editing of Instagram takeover from TU Braunschweig account

Collaboration

  • Discussion with Thorsten Seidel about FRET and suitable pairs

Dry lab

  • With instruction designing the sequencing primer
  • With instruction designing the new plasmid with the FRET sequence
  • With instruction designing the primers for NEBuilder
  • With instruction designing the primer that appends the TEV sequence

Others

  • Writing an article for BIOspektrum