We focused on collaboration with other iGEM teams. During the project, we participated in four online meets up: CCiC table conference ：hardware(held by BIT), iGEM Meeting Up (presented by CPU_CHINA, HiZJU-China, ZJUintl-China, ZJUT-China , ZJU-China), iGEM North-South Forum Conference(present by Sustech-EMB), 2022 iGEM Beijing offline meeting (presented by iBowu-China). We also participated in the offline gathering of iGEM team in Beijing hosted by iBowu-China, where we had a rare face-to-face communication with other teams during the pandemic. We also participated in the CCiC held in August and obtained the Best Presentation, Best Software and Best Genetic Element Design.

Apart from the conference, we collaborated with many teams, including BNU-China, Peking, Tongji_China, BIT, CPU_CHINA, hainanU, OUC, etc. Through the collaborations, we have also carried out a series of cooperation with CAFA_China, FAFU, HUST-China and other teams to achieve continuous collaboration.

From August 18 to August 22, the 9th Conference of China iGEMer Community (CCiC 9th), hosted by SUSTech_Shenzhen and organized with the help of CCiC Executive Committee, attracted more than 1,000 iGEMers in an online and offline collaborative format. The theme of CCiC is "Life Meets Everything", which aims to encourage people to think and explore the role of synthetic biology in different fields of research and industry, so that synthetic biology can have the power and opportunity to participate in life and change life. This is also the opportunity and challenge of synthetic biology, which embodies the vision of "Life Meets Everything".

The conference invited guests from the academic, industrial and social science fields of synthetic biology to present and exchange their achievements, views, experiences and knowledge in their respective fields with students and teachers from all over China. 77 iGEM teams presented their projects, and the iGEM Foundation and CCiC Executive Committee held a number of workshops full of useful information. Thousands of audiences participated in the event through Tencent Conference and Bilibili Live.

The conference was unprecedented in scale, with 1,224 attendees, including 85 teams and 45 individuals, covering high school and undergraduate students, who took turns to present and exchange ideas during the five-day conference. The conference also received recognition and support from iGEM headquarters. 3 iGEM guests from different departments hosted and participated in 3 lecture events, among which the Dual-use Research Workshop (Dual-use Analysis and Evaluation Workshop) at this year's conference was the first of its kind in China, leading the way.

In this conference, through the active participation of our team members, our team won three awards: Best Presentation, Best Genetic Element Design and Best Software. We also made improvements based on the shortcomings pointed out in the conference.

We participated in four online iGEM team meetups: CCiC table conference ：hardware(held by BIT), iGEM Meeting Up (presented by CPU_CHINA, HiZJU-China, ZJUintl-China, ZJUT-China , ZJU-China), iGEM North-South Forum Conference(present by Sustech-EMB), 2022 iGEM Beijing offline meeting (presented by iBowu-China).. During these meetups, we presented our project and listed the problems we encountered at that time, and discussed them with the professors and other teams. We also built up connection with BNU-China, FAFU, Tongji_China, BIT, CPU_CHINA, hainanU, OUC and other igem teams.

This was the first (and only) offline gathering of iGEMers that we attended. It was a great experience to meet face-to-face with other teams to exchange projects and explore potential collaboration opportunities.

According to the meeting process, each of iGEM teams presented their projects. Although most of the teams had met in previous online meetings, we listened to each other very carefully during the offline conference, and we had a lot of communication during discussion period. For example, we rarely had the opportunity to interact with high school teams previously because the logic of the project design was not very similar, or simply because of the working schedule. In this meeting, on the other hand, we discussed with SUSBNU_Chian about the genetic circuit they designed to produce Delphinidin, and learned about the different experiences of high school iGEM teams.

During lunch, we exchanged information with Peking and UCAS-China about the progress of their projects, the obstacles they encountered in, and became more familiar with each other. It was nice to make so many new friends in one meeting.

At the end of the meeting, iBowu-China, one of the organizers of the meeting, introduced us to the work they have done in HP and we also saw their coloring book. iBowu-China also introduced us to their idea of a mini-jamboree in October and we are looking forward to the next offline meeting.

We are actively engaged in collaboration with other teams. In the process of discussion, we also had partnership with CAFA_China, Peking, HUST-China.

With CAFA_China

Our collaboration with CAFA_China basically revolved around the topic of bio-art. We collaborated with them on the science pop-up book, as well as the TAP Synbio-art Workshop in which we both participated. Through the communication, our understanding of bio-philosophy and bio-art has been greatly improved.

With FAFU

In June, FAFU sought contact information for NEU2020 iGEMers in the iGEM China exchange WeChat group, and Tsinghua recommended the contact information to FAFU to help them advance their project.

In July, FAFU joined the Online Charity Classroom held by Tsinghua.

With HUST-China

In April, HUST-China provided plasmid that carried laccase gene for our LccDye projet.

On June 20^th, Tsinghua contacted HUST-China2021 and asked for information about the parts (cro) of HUST-China2016 team.

On August 11^th, during the communication, Tsinghua learned that HUST-China is also using the Pmr two-component system this year. In order to share our experience in the construction of the two-component system, we established a partnership with HUST-China and introduced our projects to each other in an online meeting, exchanging some experiences in wet lab, and continued to communicate in subsequent experiments.

On August 15^th, as we encountered poor stability in the construction of the Pmr two-component system, we communicated with HUST-China about experiments designed in the strength validation of the two-component system, and asked HUST-China a map of their plasmid and compare it with our plasmid to find if there was a problem in the design of the primary sequence of the receptor.

On September 5^th, in order to mutually verify the stability of each genetic components, we and HUST-China exchanged the protocols of the engineered bacteria and experiments with each other and carried out a two-week wet experiment swap.

On September 15^th, we discussed the results of the exchange wet lab.

With Peking

On July 9^th, 2022, we had a dinner meeting with the Peking team. Also, we, Peking and CAFA_China launched the TAP Synbio-art Workshop to discuss bio-philosophy and bio-art together.

With Shanghai_Tech

In February, we learned about the Shanghai_Tech 2021 project during the research of previous outstanding projects.

In April, we carried out project exchange in pre-experimental stage.

On June 3^rd, because the captain of Shanghai_Tech (Qi Xin) has participated in iGEM2021, we communicate with him to gain some team building experience.

On July 2^nd, Shanghai_Tech proposed to cooperate education and co-organize an online lecture. After communication, Shanghai_Tech participated in the Online Charity Classroom hosted by Tsinghua.

With CPU_CHINA

In April, CPU_CHINA invited us to participate in iGEM Meeting Up on May 1st, which was co-hosted by CPU_CHINA and two other universities; Tsinghua's two teams that were still in pre-experimental stage at the time. We presented our two project and received feedback from other teams.

On June 20^th, Tsinghua looked for teams in the direction of diagnosis, and received a response from CPU_CHINA, and started discussing collaboration in education.

In July, CPU_CHINA joined the Online Charity Classroom held by Tsinghua.

With Tongji

In June, we built connection at the networking session in iGEM Meeting Up, focusing on issues related to directed evolution.

With BIT

On May 28^th, because BIT is a very experienced team in hardware, we participated in their hardware roundtable activity, as our hardware was just started. We raised several issues of concern at the meeting, such as the cost of microfluidic chips, and received answers from the guest, Taiyan Zhou, who gave us a direction for our hardware design.

On July 11^th, We invited BIT to join our Online Charity Classroom, and they presented the session "Introduction to Synthetic Biology" to the audience on July 23^th, which was widely welcomed.

On July 29^th, as our hardware encountered problems in the design and iteration process of material selection and minimizing cost, we contacted Taiyan Zhou of the BIT team again and received detailed solutions, as detailed in the HP section of the exchange.

With hainanU_china

On July 21^st, since we belong to the same track, we exchanged our projects and had a meeting between the captains to explore the direction of further collaboration.

On July 22^nd, in the meeting, we discussed our understanding of each other's projects in depth. HainanU_china provided some advice on wet lab for our project. Since their project is related to Cas protein, we provided HainanU_china with the contact information of PhD students doing Cas protein modification direction in our university.

With OUC-China

On August 20^th, in the 2022 iGEM Beijing offline meeting, we learned that OUC-China's project shared similarities with ours in the dry lab, and we also hope to complete the protein-protein molecular docking to improve our model.

On August 22^nd, in the meeting, OUC-China provided the software and courses that needed to learn molecular docking, and we completed the molecular docking in our project in the follow-up work. Thanks to OUC-China ~

With BNU-China

On July 16^th, BNU was one of the teams that joined our Online Charity Classroom, where they introduced the application of biosensors, which was well received by many listeners and we had a great time working with them.

On August 24^th, BNU's wet lab design also used a two-component system, but since they had not returned to the campus to conduct wet experiments, we did not have an offline exchange program with them until near the end of the summer. During this exchange, we shared our experience in modifying the Pmr system, and they also raised some questions about the wet lab process (e.g., they found that the strip position was sometimes unstable when running glue, and we provided them with a possible explanation that the different concentrations of DNA in the samples could also cause the strips to run too slowly or too fast). The exchange with BNU was very pleasant and we are happy to have some more friends.

With SHSBNU_China

The SHSBNU_China team had some problems with modeling and wanted to seek help from the Tsinghua University team. SHSBNU_China told Tsinghua that they wanted to use modeling to speculate on the response of the sensor and its fluorescence concentration, and sent Tsinghua the equations they wanted to model. He helped SHSBNU_China to organize and analyze the equations and pointed out a more efficient way for SHSBNU_China to successfully complete the modeling. Our discussions included.

1. Helped SHSBNU_China sort out what can be used as variables and how to input them into the equations. And taught SHSBNU_China some matlab code, although the final equations were actually not reflected in the results without using matlab.
2. When the graph modeled by SHSBNU_China did not meet expectations, SHSBNU_China was instructed to change the form of the equation so that the drawn image would be more in line with SHSBNU_China's expectations and easier to understand.
3. Take SHSBNU_China through the first-order linear non-simultaneous differential equation to calculate the optimal measurement time of the receptor