Name | Type | Short Description | Length (bp) | Original Team | Contribution |
BBa_B0010 | Terminator | T1 from E. coli rrnB | 80 | Antiquity 2003 | As we expressed our parts in E. coli, we utilised it as a terminator in all our new composite parts. |
BBa_R0010 | Regulatory | Promoter (lacl regualated) | 200 | Antiquity 2003 | We extracted the final 64 bp into BBa_K4324201 and utilised it as an IPTG-inducible promoter for all our new composite parts. |
---|---|---|---|---|---|
BBa_K1602004 | Coding | Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c | 984 | TU_Darmstadt 2015 | Although we didn’t utilise it due to its low xylose affinity, we added documentation for Usage and Biology. |
Name | Type | Short Description | Length (bp) |
BBa_K4324100 | Coding | NAD(P)H-dependent D-xylose reductase from S. stipitis | 957 |
BBa_K4324101 | Coding | Xylitol Dehydrogenase from S. cerevisiae | 1071 |
BBa_K4324102 | Coding | Xylulose Kinase from E. coli | 1455 |
BBa_K4324103 | Coding | Phosphoketolase from B. lactis | 2481 |
BBa_K4324200 | RBS | RBS for E. coli | 15 |
BBa_K4324201 | Regulatory | lacPO for E. coli | 65 |
Name | Short Description | Length (bp) | Constituting Parts |
BBa_K4324000 | lacPO-RBS-XR-T1 | 1117 | BBa_K4324201 BBa_K4324200 BBa_K4324100 BBa_B0010 |
BBa_K4324001 | lacPO-RBS-XDH-T1 | 1253 | BBa_K4324201 BBa_K4324200 BBa_K4324101 BBa_B0010 |
BBa_K4324002 | lacPO-RBS-XK-T1 | 1637 | BBa_K4324201 BBa_K4324200 BBa_K4324102 BBa_B0010 |
BBa_K4324003 | lacPO-RBS-PK-T1 | 2663 | BBa_K4324201 BBa_K4324200 BBa_K4324103 BBa_B0010 |
BBa_K4324300 | XR-XDH-XK-PK | 6518 | BBa_K4324000 BBa_K4324001 BBa_K4324002 BBa_K4324003 |