Parts

Part Utilisations & Contributions

Name Type Short Description Length (bp) Original Team Contribution
BBa_B0010 Terminator T1 from E. coli rrnB 80 Antiquity 2003 As we expressed our parts in E. coli, we utilised it as a terminator in all our new composite parts.
BBa_R0010 Regulatory Promoter (lacl regualated) 200 Antiquity 2003 We extracted the final 64 bp into BBa_K4324201 and utilised it as an IPTG-inducible promoter for all our new composite parts.
BBa_K1602004 Coding Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c 984 TU_Darmstadt 2015 Although we didn’t utilise it due to its low xylose affinity, we added documentation for Usage and Biology.

New Basic Parts

Name Type Short Description Length (bp)
BBa_K4324100 Coding NAD(P)H-dependent D-xylose reductase from S. stipitis 957
BBa_K4324101 Coding Xylitol Dehydrogenase from S. cerevisiae 1071
BBa_K4324102 Coding Xylulose Kinase from E. coli 1455
BBa_K4324103 Coding Phosphoketolase from B. lactis 2481
BBa_K4324200 RBS RBS for E. coli 15
BBa_K4324201 Regulatory lacPO for E. coli 65

New Composite Parts

Name Short Description Length (bp) Constituting Parts
BBa_K4324000 lacPO-RBS-XR-T1 1117 BBa_K4324201
BBa_K4324200
BBa_K4324100
BBa_B0010
BBa_K4324001 lacPO-RBS-XDH-T1 1253 BBa_K4324201
BBa_K4324200
BBa_K4324101
BBa_B0010
BBa_K4324002 lacPO-RBS-XK-T1 1637 BBa_K4324201
BBa_K4324200
BBa_K4324102
BBa_B0010
BBa_K4324003 lacPO-RBS-PK-T1 2663 BBa_K4324201
BBa_K4324200
BBa_K4324103
BBa_B0010
BBa_K4324300 XR-XDH-XK-PK 6518 BBa_K4324000
BBa_K4324001
BBa_K4324002
BBa_K4324003