31 Jan 2022 - 10 Feb 2022: Bibliography research of relevant emerging contaminants in our country, focusing on water bodies in the Jalisco region.
11 Feb 2022 - 28/03/2022: Bioinformatic research of enzymes that can degrade selected contaminants into something we can measure.
06 May 2022 - 01 Oct 2022: Cloning EryK (BBa_K4447001) and FRET system (BBa_K4447004) into pBAD/Myc-HisB vector for protein overexpression in Escherichia coli BL21.
02 Oct 2022 - 10 Oct 2022: Analysis of protein expression through SDS-PAGE and EryK (BBa_K4447001) enzymatic activity analysis.
10 Oct 2022: Bacterial expression system (BBa_K4447005) synthesized with Twist arrived.
18 – 24 February 2022: Bibliography research of FRET systems similar to the project, including enhanced cyan fluorescent protein (ECFP) used as the donor, and Venus, used as a yellow fluorescent protein (YFP).
25 – 28 February 2022: Selection of two systems between the literature to use as a comparison.
01 – 07 August 2022: Transfer of data from the selected papers into Matlab and search of models that best fit the data.
08 – 14 August 2022: Interpretation of results and comparison of parameters.
15 – 21 August 2022: Written explication of results.