Parts

Overview

Throughout the year, we have been trying to obtain antimicrobial peptides from eukaryotic organisms in bacteria. In addition, we have designed constructs to obtain double-stranded siRNAs that can help us reduce the pathogenicity of wilt-causing organisms. On this page you can see the parts we created, the parts we use and the expression cassettes we designed.

Accession number	Description	Purpose	Length (pb)	Status
Basic parts
BBa_K4147000	An antimicrobial peptide from Piper colubrinum (PcOSM)	To have the sequence of the antimicrobial peptide.	711	In progress
BBa_K4147001	An antimicrobial peptide from Phyllomedusa bicolor that contains a tandem repeat of a chitin binding domai (CBD) as an improvement. (CBD)2-DrsB1	To improve the antifungal efficiency of dermaseptin in the registry.	711	In progress
BBa_K4147016	siRNA designed to target RXLR gene of P. capsici.	To suppress the RXLR gene in P. capsici to decrease its pathogenicity.	18	In progress
BBa_K4147014	Reverse of BBa_K525998	To produce the double-stranded siRNAs.	32	In progress
BBa_K4147015	Reverse of BBa K864600	To produce the double-stranded siRNAs.	18	In progress
BBa_K4147006	Disulfide interchange protein (DsbA) from Pseudomonas aeruginosa	To enhance the formation of disulfide bridges of antimicrobial peptides by expressing them in E. coli BL21 (DE3).	636	In progress
BBa_K4147017	siRNA against ODC gene	To produce the double-stranded siRNAs.	18	In progress
BBa_K4147009	MsrA2 antimicrobial protein for purification	To produce MsrA2 fused with 6X-Hist tag for purification by metal affinity chromatography	117	In progress
Composite parts
BBa_K4147002	Expression cassette for (CBD)2-DrsB1 peptide	To produce the antimicrobial peptide (CBD)2-DrsB1 in E. coli BL21 (DE3).	1075	Ligated
BBa_K4147003	Expression cassette for PcOSM peptide	To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3).	1054	Ligated
BBa_K4147007	Expression cassette for PcOSM peptide with DsbA	To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3) while enhancing disulfide bridge formation.	1738	Ligated
BBa_K4147008	Expression cassette for (CBD)2-DrsB1 peptide with DsbA	To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3) while enhancing disulfide bridge formation.	1738	Ligated
BBa_K4147010	Expression cassette for mCherry with BBa_R0010 promoter	Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct.	1738	Ligated
BBa_K4147011	Expression cassette for mCherry with BBa_R0011 promoter	Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct.	1738	Ligated
BBa_K4147004	Expression cassette for DrsB1 construct with lacI promoter	To produce the antimicrobial peptide DrsB1 in E. coli BL21 (DE3).	481	Ligated
BBa_K4147012	Up part of cassette for siRNA production of RXLR gene for P. capsici	Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct.	118	In progress
BBa_K4147013	Down part of cassette for siRNA production	Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct.	92	In progress
Existing parts used
BBa_R0011	Hybrid region regulatory promoter. Consists of phage lambda promoter P(L) with cl binding sites replaced by lacO1. Can be repressed by LacI inhibitor and can be induced by IPTG.	Express antimicrobial peptides and test our model.	55	Works
BBa_R0010	LacI regulated promoter. It can be induced by IPTG.	Express antimicrobial peptides and test our model.	200	Works
BBa_B0032	Weak RBS based on Ron Weiss's thesis	Express antimicrobial peptides.	13	Works
BBa_B0015	Double terminator	Express antimicrobial peptides.	13	Works
BBa_K525998	T7 promoter and RBS	siRNA production.	20	Works
BBa K864600	Double terminator	Express antimicrobial peptides.	52	Works
BBa_K2577001	MsrA2 antimicrobial protein	To compare the fungal activity of this protein alone compared to that fused with CBD (BBa_R0001).	96	In progress