Nowadays, many people face the problem of incomplete digestion of fats in food, which can lead to health problems such as malnutrition. In our project, we used genetic engineering techniques to create bacteria that overexpress lipase. Lipase is an enzyme that helps digest fats in the body’s small intestine, which means that it may provide some relief from this health problem. We performed experiments to test enzyme activity under different conditions, helping to confirm the possibility of incorporating this technology into human practice. In the future, our project could be a dietary supplement used in a probiotic drink to benefit people who help digest fat.