CRISPR-Cas9 is the third-generation gene editing technology after the introduction of ZFN, TALENs and other gene editing technologies. In just a few years, it has swept the world and become the most mainstream gene editing system today. As research progresses, CRISPR gene-editing technology carries an implied risk of off-target, the accidental editing of other genes in the genome with unpredictable consequences. In this project, the common tool plasmids in prokaryotic expression systems are transformed, and TSD and TIR elements that can be recognized by Casposons are added to achieve site-directed insertion, which is verified by sequencing. This technology can not only play a role in the study of disease treatment methods, but also become an efficient tool for upstream gene cloning.