Oral Vaccines
·Non-proprietary Name: Combined vaccine (a combination of Enterovirus Type 71 Vaccines and Rotavirus Vaccine)
·Suitable age: 3 to 5 years
·Dosage: 50ml
·Dosage form: Lactobacillus
·Optimum shelf life: 12 months-18months at 35.6~46.4℉(2~8℃)
Take orally
(If you need more information,read the instruction book)
It can relieve neurological disease and HFMD in children. It can also relieve diarrhoeal disease and gastroenteritis.
·Ask the doctor before taking the vaccine
·Make sure child is in good health
·Don’t eat&drink within half an hour before&after taking the vaccine
·Avoid food that is easy to be allergic to before and after vaccination
·Eat a bland date and avoid spicy food
·Stay in the hospital for half an hour to make sure the child isn’t unwell
Fever
Diarrhea
Dizzy
Headache
Nausea
Vomit
Lassitude
Cough
Firstly, the expression of EV71 vp1-RV VP7 fusion protein and vaccine adjuvant IL-12 was confirmed by the E. coli expression system; Then, the Bifidobacterium expression engineering bacteria were constructed, and the secondary vaccine was prepared. The fluorescence imaging was used to confirm that vp7-vp1 was negatively located on the envelope, and the expression level of EV71 vp1-vr VP7 fusion protein was confirmed. Finally, the specific antibody against vp7-vp1 was induced in the body of the user at the same time to effectively protect them from the infection of these two types of viruses.
The first column is the marker, which can help us measure the length of protein when we compare those bands to it. The second to seventh columns are unpurified proteins. Because they have not been purified, there are many hetero bonds. The six columns on the right are the purified proteins, and basically, only the content we need is left. In the unpurified protein, even if there are many hetero bonds, the protein can still be expressed successfully, but in the purified protein, we can see the protein expression more intuitively.
Bifidobacterium in Lactobacillus is not only an important probiotic to maintain the stability of human intestinal flora, but also an ideal expression vector for the oral live vaccine. Relevant studies have found that it has a more significant preventive effect on gastrointestinal pathogens. Lactobacillus is safe and endotoxin free. The expressed foreign protein can be taken directly with the bacteria without purification and can be used to prepare the oral vaccine. We can take the ptrc99a vector as the skeleton again, construct the recombinant expression plasmid, transform it into a Bifidobacterium system, detect its expression, and evaluate the feasibility of preparing an oral vaccine reagent.
