Enteroviruses and rotaviruses are the main gastrointestinal pathogens affecting the health of infants and young children. This project intends to target two viruses, explore dual oral vaccines, and use the bifidobacteria expression system to obtain recombinant proteins expressed by fusion of two antibodies, so as to achieve immunity from two viruses by eating a sugar pill, and protect the health of infants and young children. The principle is to construct a VR-vp7 and EV71-vp1 fusion expression vector, import it into Escherichia coli BL21 (DE3), and then transfer it into Bifidobacterium after expression verification to verify the possibility of constructing a rotavirus/EV71 combined antibody. The oral vaccine has broad clinical application prospects.
