Parts

Overview

Our part consists of constitutive promoters for screening signal peptides, inducible promoters for overexpression of enzymes, signal peptides for secreting enzymes, and genes encoding cellulose degrading enzymes. Plasmid construction was performed using a classic molecular cloning technique. Promoters are cut with BglII and NdeI and inserted into the vector, the secretion signal is cut with NdeI and NcoI, and the enzyme with NdeI and XhoI can be used for cloning. All DNA fragments for the parts we used were synthesized from IDTDNA.