1.Break the ice

Get to know each other by asking questions and introduce oneself to the others

2.Run for the captain of the team

People who wished to be the captain each made a speech in front of all the members

3.Learn the basic knowledge

4.Design the name and icon of the team

1.Learning all the instruments in the lab

2.Extract DNA from poplar leaves

3.Clone target genes by PCR

4.Run agarose gel electrophoresis to check the gene

5.Recycle PCR products in the gel

1.Learn the knowledge about the vector construction

2.Construct the clone vector

3.Escherichia coli genetic transformation

4.Foster the cells in solid medium

1.Use the colony PCR and run agarose gel electrophoresis to check whether the vector construction is successful

2.Foster the cells in LB liquid after checking

1.Visit Beijing Zhongguancun Biology Bank

2.Run gel with the gene of interest and combine it with the expression vector

3.Foster the expression vector in the LB liquid

1.Prepare the PCR system

2.Construct the gene of interest MYB-46 with expression vector

3.Foster the vector

1.Extract plasmid

2.Transform the yeast receptive state.

3.Prepare the poplar leaves

4.Infect the poplar leaves with the expression vector

1.Run pcr for the vectors

2.Run agarose gel electrophoresis to check the useful parts

3.Recycle them and use lead to check its ability of bearing the lead

1.Learn how to get the gene sequence of the gene

2.Prepare for the online speech about our programme

3.Vote to choose the captain of the whole team and the captain of the wet lab and dry lab