We aim is to use synthetic biology technology to absorb lead ions in soil to repair the land while letting the poplar more robust growth. This will help to solve the problem of environmental pollution in the soil. To better implement our project, we made the following analysis to better implement in the product in the real world.

This project will be beneficial in improving the ecological environment and restoring the soil. For this purpose, our project's ultimate beneficiaries are those who live in the area around the mine, and those who live in the lead-contaminated area. At the same time, we also need to communicate and cooperate with relevant government departments in planting vegetation so that the project can be put into the real world more smoothly. We plan to establish pilot projects by communicating with environmental protection companies in areas where the disaster is more severe. After planting poplars, we will regularly share with relevant partner companies, collect soil samples every month, and analyze the content of lead ions in the soil to calculate the actual absorption efficiency. At the same time, we will also send personnel to observe the growth of the poplars to ensure the absorption of heavy metal ions while monitoring the growth status of poplars. This will help improve the appearance of the local natural environment and vegetation coverage rate.

The safe use of synthetic biology is essential. Therefore, safety is also our top priority. First, we strictly follow the experimental biological requirements during the experiments and when preparing the safety forms. We also selected harmless strains for humans and the environment for experiments and implementation. When launching the project, we also need to consider some safety issues. For example, whether the modified poplar genes will harm the growth of poplar trees. We have not found such problems in the current experiment, but we will further ensure the safety of the investigation and delivery by regular observation of poplar genes and cells. At the same time, considering the public's fear of lead ion heavy metal pollution, we will send special personnel to plant the poplar and train the personnel to maintain the poplar. At the same time, synthetic biology is a new discipline, and the public is not aware of the overall implementation safety of synthetic biology. Therefore, we attach great importance to further public publicity and education on the safety of synthetic biology. We will also continue to hold online and offline lectures to popularize the safety of synthetic biology.

We may encounter other challenges as we implement the project. For example, heavy metal ions can enter the plant after it adsorbs heavy metals. Therefore, if poplar leaves fall to the ground and flutter in the wind, it will increase the pollution area. At present, we take two methods to avoid it. First, we test the areas where poplar planting is more polluted and recycle the leaves in time to reduce secondary pollution. Second, we are further developing related technologies to inhibit the entry of heavy metal ions into the leaves, so there is no need to worry about the secondary pollution caused by falling leaves.

In addition, since gene editing technology is still in its infancy, people may be conservative about using gene editing technology for soil remediation. Therefore, in the implementation, we should pay attention to and record data promptly and actively communicate with the government and the public. While popularizing knowledge, we should let stakeholders see that the advantages outweigh the disadvantages.