Heavy metal tolerance analysis of PtoMYB46 in yeast cells

1. The correct yeast transformants ycf1-pYES2-PtMYB46, ycf1-pYES2, BY474-pYES2-PtMYB46 and BY474-pYES2 were transferred into liquid SD/Ura.

2. 100 uL yeast liquid was inoculated into SD/Ura (2% galactose + (CH3COO)2Pb 20mmol·L-1) medium containing different concentrations of heavy metals in 50mL conical flask, and OD was adjusted to 1.0.

3. 30℃, 250r·min-1, foster the yeast cell.

4. Samples were taken at different time points (0,6, 12, 24, 30 h), and the OD600 value was measured to make the growth curve.