Results

acetate production

acetate concentration with 1.5mM IPTG (1).png
As shown in the graph, acetic acid was synthesized when 1.5 mM IPTG was added.

OD600 time.png
OD600 has a higher growth rate at higher IPTG concentrations.

cellulose degradation

20221011.png
In insert, there is a trend toward increased glucose levels up to 6 hours, while there is no significant trend in CONTROL After one night of incubation, glucose levels in both cases have decreased significantly. This is assumed that the rate of cellulose degradation exceeded the rate of glucose consumption, as a result of E. coli growth and more glucose being used as a nutrient source, while cellulose was precipitated and was not degraded as much.

Measuring the pH of the culture

figure.1.jpg
ph increased regardless of whether iptg was added.

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