This transporter could contribute to raising the pH of the culture fluid. In our experiments, this part was assembled to E.Coli, BL21 strains with poxB, which catalyzes the decarboxylation of pyruvate to make acetate ion and CO2 and is induced by IPTG.(BBa_K4206001) the genetically modified E.coli are cultured and induced with 1mM IPTG. After 12 or more hours, we measured the pH of the culture fluid based on pH test paper. The measurement result was pH 8-9, both with and without IPTG induction. Therefore, this transporter could transport acetate ions. Considering growth limits of E.coli, ph increase needs attention.
0:IPTG0mM 1:IPTG1mM