Safety is always the most important during the experiment. Since doing the experiments, we have strictly followed the security policies required by iGEM. So in the whole experiment, safety has been completely guaranteed.
Our experiments are conducted in the ATLATL laboratory, and the entire laboratory is strictly equipped with safety equipment to protect our safety. The laboratory is equipped with cabinets for storing experimental reagents, as well as a fire extinguisher and a laboratory eyewash.
Before the experiment started, our entire team took part in an online course on biosafety. We learned how to operate these experimental devices safely during experiments, and what to do when an accident occurs.
Our experiments were performed in the BSL-1 laboratory. Biosafety Level 1 (BSL-1), is suitable for laboratory environments where personnel dealing with low-risk microorganisms pose little threat of infection to the human body. During the experiment, we use various experimental instruments as standards and strictly abide by the safety standards. For example, when we use the centrifuge to centrifuge the reagents, we will put them into the same reagent tubes for balancing to ensure the safe operation of the centrifuge. At the same time, we also strictly abide by the following safety instructions when entering the laboratory:
We comply with the above safety requirements throughout the experiment and strictly abide by the iGEM laboratory safety standards.
During the experiment, the enzymes we used are all safe, active, and stable, including KOD-plus-neo, NcoI, and BamHI. Other materials, such as peptone, yeast powder, 1*TAE buffer, agarose, etc., are uncontaminated and pose little risk to our health. During the experiment, we were all under the supervision of the instructor, and all equipment, such as petri dishes, beakers, and measuring cylinders, were cleaned or sterilized before use.
