Safety was a critical part of our wet lab practices.
We underwent thorough safety training that included proper microbiological techniques, waste, and accident management.
We were introduced to the equipment we were going to be using, and the dangers of various commonly used chemicals were emphasized.
We were also taught how to manage possible accidents and spills. The laboratory dress code was strictly followed, which included wearing closed shoes ,
full-length trousers, gloves and wearing a lab coat when spectating. The lab coats were stored in a drawer in the lab to avoid contamination.
This minimized any risks of hazard to ourselves, and any accidental release of microorganisms in the environment.
We paid very close attention to waste management. This is often the way microbes and toxic chemicals are released into the environment by experiments.
The microbiological assay was very useful in our short stay in the lab. This is why we took such care in setting up these protocols.
An aseptic technique was always used in our experiments to ensure the reproducibility of our results and the prevention of microbial contamination.
All of our microbiological work was done in a biosafety cabinet and under flame to ensure no E.coli release.
We took special care when using ethidium bromide during the electrophoresis, because of it is cancerous action.
All the workstations were disinfected with 70% ethanol both before and after each experiment.
When working with microorganisms, nitrile gloves were worn to protect the user's hands.
In addition, we were particular about not unfairly disposing of living microorganisms.
Our used Petri dishes were autoclaved before being discarded, and all liquid culture were incubated overnight with a strong chlorine/soap solution,
before disposal.
The safety of our research was a top priority in our selection of chassis and organisms.
That ‘s why we chose E. coli DH5-Alpha, very common engineered laboratory microorganisms, with no risk of human pathogenesis.
Our final product will be a biological biocide, which major component will be the antimicrobial peptide, tachyplesin.
Natural tachyplesin has hemolytic action, so we will use its analog I11S, with very limited hemolytic action.
In any case, our product will be used by specialized preservers, who will apply the biocide wearing robes, masks and gloves.