As a professor at The Ohio State University, Dr. Wood has a lab on campus. When we needed to start working in the lab, but hadn't found a new lab space to host us, Dr. Wood allowed us to work in his lab.
As a graduate student in Dr. Wood’s lab, Sophia was a huge help. Alongside her own personal research, Sophia helped guide us in the cloning process.
When we needed help, namely a PI for our organization, Dr. Shearer stepped up and said he would be our PI, despite his full schedule. As a PI he has been invaluable, helping us navigate fund transfers, performing required iGEM PI duties, and overall just being there for us.
Nathan is also a graduate student in Dr. Wood’s lab. He helped us plan and design our wet lab experiments.
After graduating in the spring of 2022, Lindsey stuck around with the team as an advisor. Her 2 years of iGEM experience proved valuable with her providing general support and advice through general body meetings. She also had many ideas on how to effectively manage time and she took time to help all of us edit our wiki writeups.
Yingyi also graduated in the spring of 2022 and was on the 2021 OhioState iGEM team. Throughout the spring she attended general body meetings, adding to our ideas, and raising important questions.
Dr. Lenski provided us with a pathway to creating an unsteady state model in Matlab by suggesting we try solving the differential equation using the Runge-Kutta method.
Dr. Harsch helped us choose experiment ideas for both Boonshoft and COSI while giving advice on aspects that would make the experiments ideal for younger generations.
Dr. Lynes helped educate the team on what it is like to go through and survive sepsis. He brought to light how sepsis doesn’t just ravage the body but also the mind, with many sepsis patients experiencing ICU psychosis.
They were our main contacts with COSI. Laurie helped decide on a date for our display along with a location while Amy gave us a tour of our presenting space and was there for the the actual event. They worked with others at COSI to ensure the community knew of our event.
Dr. Ahmer provided wet lab advice on our cloning process. He suggested we save time and resources by cloning genes into our bacteria before we progress and attempt to clone into phage.
Dr. Coopersmith further elaborated on ICU psychosis and life after sepsis. He spoke to us about challenges after sepsis and how sepsis knowledge is key to its treatment. Even one person who understands what sepsis looks like can save someone’s life.
Dr. Levy was able to provide us with general sepsis information. He also pointed out some interesting knowledge about how some patients actually develop immunosuppression throughout the course of sepsis.
Jordan was our main contact with Boonshoft. He helped us decide where to conduct our experiments and put our displays.
We worked closely with the 2022 Wright State team and their advising team when constructing and figuring out what projects we wanted to do for our exhibit at Boonshoft and COSI. This led to a successful turn out at both facilities and an increase in awareness of synthetic biology.
The 2022 OhioState iGEM team continued a derivative project design based on last year’s team, the 2021 OhioState iGEM team. We continued the idea of phage therapy to treat sepsis; however, we expanded this phage approach to a phage reporter assay to help detect sepsis. In this way we are both finding and fighting sepsis! Some wiki styles and designs were also adapted from last year as Ryan Burrows, the primary wiki coder, remained on the team.
As a part of wet lab, he specifically began by looking into the biosynthesis of Lipid A. He continued by working with wet lab throughout the summer, devoting most of his time there.
He was the president for this year after being on the team last year. He oversaw general meetings and took part in dry lab, coding the phage cocktail model. He also took part in human practices where he helped organize the Boonshoft and COSI events. Ryan was also the primary wiki coder, creating and designing the team’s wiki. Overall he was the project administrator, meaning he managed and coordinated project activities, planned out the year, and made sure all of those plans were executed.
Satvik headed the wet lab committee. He was in charge of planning and everything that had to be done for wet lab. Satvik was our go to science brain and was heavily focused on the intricate details and happenings of the wet lab, completing the majority of lab work himself.
Angela headed the human practices committee. She led the outreach portion of the project at the Boonshoft and Center of Science and Industry (COSI) science museums in Ohio. She also took part in interviews with past sepsis patients and doctors who have had extensive experience with sepsis. Angela was in charge of the visualizations for Instagram where she posted updates on the project and allowed viewers to learn more about the team. Beyond Instagram, Angela also helped communicate our project by being one of the main wiki content writers and editors.
Joey was involved with dry lab and helped with project ideation.
Elijah was involved with the wet lab committee. He designed strong promoter sequences with help from Satvik, and tested 5 strong promoters to optimize the reporter phage. Elijah also was a huge help in drafting content to be put onto the wiki.
Fesume headed the dry lab committee. He led the development of the phage-bacteria population model and the bioreactor design. Fesume helped with the preliminary research for the sepsis detection device. He was involved with HP, with his main contribution being the creation of the phage puzzle. He also provided some assistance developing the wiki.
Kaya was involved with wet lab, created the pricing list for materials, and helped a bit with planning some meetings. He helped organize the Notebook, read through, and ultimately edited some wiki pages.
Emily was involved with dry lab where she worked on designing the sepsis detection device as well as expanding the phage database for different bacteria. She also took part in human practices by reaching out and meeting with various physicians and patients.
We would like to thank Integrated DNA Technologies, Ohio State's Infectious Diseases Institute, Ohio State's College of Engineering, and Ohio State's College of Food, Agricultural, and Environmental Sciences for their generosity in helping finance our team.