2021
December
●Hold a biology contest to select team members for NUDT_CHINA.
2022
January
●NUDT_CHINA set-up. Team members split into 3 groups:Lab, Human Practice, Art&design.
February
●Conduct a questionnaire survey among more than 400 students.
●Brainstorm for 2022 team project.
●Learn the molecular biology and basic experimental technologies.
March
●Determine the direction of the 2022 iGEM project, consider to choose glococorticoids as our stress marker.
●Conduct preliminary Proof of Concept design and plasmid design.
April
●Collection and study of relevant literature on the application of our project.
●Hold online meeting with Fudan.
May
●Choose GRE as the module that respond to glucocorticoid stimulation.
June
●Offline meetings of the experimental group for literature reading, reporting the progress of the project and arranging the follow-up tasks.
July
●Hold online meeting with CSU_CHINA.
●Online meetings of the experimental group for literature reading and plasmid design.
●Tried 3-copy GRE sequence and 6-copy GRE sequence in order to increase the response to glucocorticoid stimulation.
●Team uniform&project logo design.
August
●Conducting relevant experimental basic learning, including software design and skill training offline.
●Use EGFP to characterized the subcellular lolcalization of GR.
●Combine the advice and the feedback from literature to redesign the Proof of Concept to the right direction of improvemnet in increasing the response to glococorticoid stimulation.
●Construct the expression plasmid of EGFP and LBD fusion protein.
●Replace the lost DBD domain by using the DNA binding function of tetR.
●Promotion video production.
September
●Conduct offline Communication meetings with CSU_CHINA and reach consensus on deep cooperation in collaboration, education and science communication.
●Establish partnership with CSU_CHINA.
●Interview experts and receive optimization suggestions.
●Introduce Synthetic Biology to teenagers on the National Science Popularization Day in Changsha.
●Hold a disscussion with a cell biologist.
●Successfully complete the Proof of Concept and carry out the experimental design of Demonstration.
●Construct tdTomato and TYR expression plasmids regulated by tetO promoter.
●Complete the Demonstration experiment, feed the results back to Model group, and carry out the optimization design of experiment.
●Conduct optimized experiment with VP64 combined with NES and different length of linkers we designed.
●The optimized Demonstration experiment obtain better effect.
October
●Taking family photos.
●Conduct doctoral student interviews, research literature on application form and interview with clinical psychologist.
●Wiki construction.
●Presentation Video Production.
●Participation of Giant Jamboree.