designed by Melike Sabry

Parts

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Parts by iGEM Munich

brought to you by Till & Rosa
Please note for people with a leading * they might not necessarily be the original creator, but the sequene was taken from the given literature.

Basic Parts

Name Type Description Designers Length
BBa_K4497000 CDS pH-Linker Wu, Y. J., Fan, C. Y., & Li, Y. K. (2009). Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877(31), 4015–4021. https://doi.org/10.1016/J.JCHROMB.2009.10.009 75 bp
BBa_K4497001 CDS Kappa CL Nicolas Blömeke 318 bp
BBa_K4497002 CDS Anti-GFP nanobody Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7 342 bp
BBa_K4497003 CDS Interleukin-11 receptor alpha chain signaling sequence *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 69 bp
BBa_K4497004 CDS CD28 transmembrane domain *Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g 63 bp
BBa_K4497005 CDS TEV Protease Kapust, R. B., Tözsér, J., Fox, J. D., Anderson, D. E., Cherry, S., Copeland, T. D., & Waugh, D. S. (2001). Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Engineering, 14(12), 993–1000. https://doi.org/10.1093/protein/14.12.993 711 bp
BBa_K4497006 CDS TEV recognition sequence Kapust, R. B., Tözsér, J., Fox, J. D., Anderson, D. E., Cherry, S., Copeland, T. D., & Waugh, D. S. (2001). Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Engineering, 14(12), 993–1000. https://doi.org/10.1093/protein/14.12.993 21 bp
BBa_K4497007 CDS Tetracycline-Induced Transactivator tTA Kafri, T., van Praag, H., Gage, F. H., & Verma, I. M. (2000). Lentiviral vectors: Regulated gene expression. Molecular Therapy, 1(6), 516–521. https://doi.org/10.1006/mthe.2000.0083 1005 bp
BBa_K4497009 CDS IL27alpha Secretion Sequence Nicolas Blömeke 84 bp
BBa_K4497012 Tag Flag-Tag *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 21 bp
BBa_K4497013 CDS Anti-mCherry Nanobody Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., Oeffinger, M., Nussenzweig, M. C., Fenyö, D., Chait, B. T., & Rout, M. P. (2014). A robust pipeline for rapid production of versatile nanobody repertoires. Nature Methods 2014 11:12, 11(12), 1253–1260. https://doi.org/10.1038/nmeth.3170 375 bp
BBa_K4497015 CDS Linker 1 MESA Till Gundlach 21 bp
BBa_K4497016 CDS Linker 2 MESA Till Gundlach 90 bp
BBa_K4497026 Tag 6xHis-Tag Nicolas Blömeke 33 bp
BBa_K4497027 Regulatory Bidirectional TRE-CMVmin Promoter Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g 495 bp
BBa_K4497029 Reporter miRFP680 Matlashov, M. E., Shcherbakova, D. M., Alvelid, J., Baloban, M., Pennacchietti, F., Shemetov, A. A., Testa, I., & Verkhusha, V. v. (2020). A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Nature Communications, 11(1), 1–12. https://doi.org/10.1038/s41467-019-13897-6 951 bp
BBa_K4497031 Reporter mCherry *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 696 bp
BBa_K4497032 Domain T2A Self Cleaving Peptide Wang, Y., Wang, F., Wang, R., Zhao, P., & Xia, Q. (2015). 2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyx mori. Scientific Reports, 5(1), 1–10. https://doi.org/10.1038/srep16273 54 bp
BBa_K4497034 Reporter mEGFP *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 717 bp
BBa_K4497035 CDS TEV recognition sequence (S) *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 21 bp
BBa_K4497036 CDS Fc Domain *Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 699 bp

Composite Parts

Name Type Description Designers Length
BBa_K4497008 Coding GFP Nanobody Receptor Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 450 bp
BBa_K4497010 Coding tTA Signaling MESA Part Schwarz, K. A., Daringer, N. M., Dolberg, T. B., & Leonard, J. N. (2017). Rewiring human cellular input-output using modular extracellular sensors. Nature Chemical Biology, 13(2), 202–209. https://doi.org/10.1038/nchembio.2253 1845 bp
BBa_K4497011 Coding TEV MESA Part Schwarz, K. A., Daringer, N. M., Dolberg, T. B., & Leonard, J. N. (2017). Rewiring human cellular input-output using modular extracellular sensors. Nature Chemical Biology, 13(2), 202–209. https://doi.org/10.1038/nchembio.2253 777 bp
BBa_K4497014 Coding mCherry Nanobody Receptor Engelowski, E., Schneider, A., Franke, M., Xu, H., Clemen, R., Lang, A., Baran, P., Binsch, C., Knebel, B., Al-Hasani, H., Moll, J. M., Floß, D. M., Lang, P. A., & Scheller, J. (2018). Synthetic cytokine receptors transmit biological signals using artificial ligands. Nature Communications, 9(1). https://doi.org/10.1038/S41467-018-04454-8 507 bp
BBa_K4497017 Coding MESA GFPnb-L1-tTA Till Gundlach 2316 bp
BBa_K4497018 Coding MESA GFPnb-L1-TEV Till Gundlach 1264 bp
BBa_K4497019 Coding MESA mCherrynb-L1-tTA Till Gundlach 2373 bp
BBa_K4497020 Coding MESA mCherrynb-L1-TEV Till Gundlach 1305 bp
BBa_K4497021 Coding MESA GFPnb-L2-tTA Till Gundlach 2385 bp
BBa_K4497022 Coding MESA GFPnb-L2-TEV Till Gundlach 1317 bp
BBa_K4497023 Coding MESA mCherrynb-L2-tTA Till Gundlach 2442 bp
BBa_K4497024 Coding MESA mCherrynb-L2-TEV Till Gundlach 1374 bp
BBa_K4497025 Coding Kappa CL Tetramer Rosa Reithmeir, Nicolas Blömeke 1464 bp
BBa_K4497028 Reporter tTA Induceable EYFP Reporter Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g 1212 bp
BBa_K4497030 Reporter tTA Induceable miRFP Reporter Rosa Reithmeir, Till Gundlach 1446 bp
BBa_K4497033 Composite tTA Induceable 2xmCherry T2A EYFP Till Gundlach 2814 bp
BBa_K4497037 Coding GFP-Fc Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804. https://doi.org/10.1371/JOURNAL.PONE.0230804 1575 bp
BBa_K4497038 Coding mCherry-Fc Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804. https://doi.org/10.1371/JOURNAL.PONE.0230804 2415 bp
BBa_K4497039 Coding GFP-mCherry-Fc Mossner, S., Phan, H. T., Triller, S., Moll, J. M., Conrad, U., & Scheller, J. (2020). Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands. PLOS ONE, 15(4), e0230804. https://doi.org/10.1371/JOURNAL.PONE.0230804 2283 bp
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