wiggly membrane

Parts

wiggly membrane

Table

Registry Part Name Name of Part Type Description
BBa_K4514001 IGEM002 Primer Used in the tail fiber plasmid
BBa_K4514002 IGEM003 Primer Forward primer: ori
BBa_K4514003 IGEM003b Primer Used in the Truncated tail fiber and Tail fiber plasmids
BBa_K4514004 IGEM004 Primer Used in the Truncated tail fiber and Tail fiber plasmids
BBa_K4514005 IGEM008 Primer Used in the Truncated tail fiber plasmid
BBa_K4514006 IGEM009 Primer Used in the nanobody Gblock plasmid
BBa_K4514007 IGEM009b Primer Used in the nanobody Gblock plasmid
BBa_K4514008 IGEM010 Primer Forward primer for Nanobody and His tag
BBa_K4514009 IGEM011 Primer Used in the nanobody Gblock plasmid
BBa_K4514010 IGEM012 Primer Used in the nanobody Gblock plasmid
BBa_K4514011 IGEM013 Primer Used in the nanobody Gblock plasmid
BBa_K4514012 IGEM014 Primer Used in the nanobody Gblock plasmid
BBa_K4514013 IGEM015 Primer Used in the nanobody Gblock plasmid
BBa_K4514014 IGEM016 Primer Reverse primer for nanobody
BBa_K4514015 IGEM018 Primer Forward primer for nanobody
BBa_K4514016 IGEM024 Primer Reverse primer for polyglycine
BBa_K4514017 IGEM025 Primer Reverse primer for nanobody
BBa_K4514018 IGEM026 Primer Forward primer for nanobody
BBa_K4514019 IGEM027 Primer Reverse primer for gp17 with tag
BBa_K4514020 IGEM028 Primer Forward primer for gp17 with tag
BBa_K4514021 IGEM029 Primer Forward primer for nanobody
BBa_K4514022 IGEM030 Primer Reverse primer for nanobody
BBa_K4514023 Sortase forward Primer Forward primer for pET29 template
BBa_K4514024 Sortase ori forward Primer Reverse primer
BBa_K4514025 Sortase ori reverse Primer Forward primer
BBa_K4514026 Sortase reverse Primer Reverse primer for pET29 template
BBa_K4514027 gp12 coding T7 phage tail protein
BBa_K4514028 gp11 coding T7 phage tail protein
BBa_K4514029 gp17 coding T7 phage tail protein
BBa_K4514030 AmpR coding Antibiotic resistance cassette
BBa_K4514031 SP6 promoter promoter Bacteriophage promoter
BBa_K4514032 M13 rev other Recognition site for sequencing
BBa_K4514033 lac operator regulatory Repressor recognition site
BBa_K4514034 lac promoter promoter Bacteria promoter
BBa_K4514035 CAP binding site regulatory Activator binding site
BBa_K4514036 ori other Origin of replication
BBa_K4514037 AmpR promoter promoter Antibiotic resistance cassette promoter
BBa_K4514038 f1 ori other Bacteriophage origin of replication
BBa_K4514039 Kozak sequence regulatory Translation inititation site
BBa_K4514040 M13 fwd other Recognition site for sequencing
BBa_K4514041 Sortase Tag tag Peptide tag
BBa_K4514042 T7 promoter promoter Bacteriophage promoter
BBa_K4514043 RBS(KanR) RBS Antibiotic resistance cassette ribosomal binding site
BBa_K4514044 Nanobody 2 (Nbdy2) in pET29 Plasmid Nanobody on a sortase plasmid backbone
BBa_K4514045 pGEM3RCF-T7 LEPTGG tail fiber truncated Plasmid Truncated T7 tail fiber with sortase tag
BBa_K4514046 pGEM3RCF-T7-LPETGG Tail fiber Plasmid Regular T7 tail fiber with sortase tag
BBa_K4514047 T7 Packaging Signal regulatory Gene in Cas13a that packages it into the T7 capsid
BBa_K4514048 pGEM3RCF - T7 TailFiber plasmid This is a backbone plasmid with an ampicillin resistance gene, T7 promoters, and a lac regulator.
BBa_K4514049 pDSG289-nanobody 2 intimin Plasmid Backbone plasmid for nanobody(fusion)
BBa_K4514050 pGEM3RCF-T7 LPETGG-GGG-Nanobody Fusion Plasmid Truncated T7 with Nanobody
BBa_K4514051 gp17_truncated coding Truncated bacteriophage tail fiber
BBa_K4514052 CRISPR-Cas13a expression vector Plasmid This is an expression vector for CRISPR-Cas13a expression in E. coli. The plasmid contains the type VI CRISPR effector from Leptotrichia shahii with RNA-guided RNAse activity (Abudayyeh et al., 2016), a T7 promoter, a T7 insert, a T7 packaging signal, ori ColE1, a kanamycin resistance cassette, and the f1 origin of the M13 phage (Kiga et al., 2020).
BBa_K4514053 Expresses the antigen that pDSG289 binds to. Plasmid This plasmid codes for antigens that the nanobody from pDSG289 attacks. The newly made nanobody from either the fusion plasmid or the sortase method will still attack this antigen.
BBa_K4514054 Plasmid backbone for T3 Plasmid This plasmid codes for the gp17 tail fiber gene for the T3 bacteriophage. It also codes for Kanamycin resistance, gp11, and gp12.
BBa_K4514055 Added the sortase tag for T3 Plasmid This plasmid codes for the gp17 tail fiber gene for the T3 bacteriophage. It also codes for Kanamycin resistance, gp11, gp12, and the sortase tag. This was transformed into DH5-alpha E. coli.
BBa_K4514056 Nanobody g-block gene added to sortase backbone Plasmid A plasmid containing the evolved version of sortase A (eSrtA) backbone. eSrtA has been improved to exhibit, up to an 140-fold, increase in activity and kinetics (Chen et al., 2011). eSrtA is cloned, transformed, and expressed in E. coli BL21.
BBa_K4514057 Reverse primer for T3 gp17 primer This primer was to add the sortase LPETGG tag onto the T3 gp17 phage tail fiber
BBa_K4514058 Reverse primer for T3 truncated gp17 primer This primer was to add the sortase LPETGG tag onto the T3 truncated gp17 phage tail fiber
wiggly membrane