1. Generation of DNA fragments
2. Construction of plasmids by in vivo recombination of DNA fragments with overlapping ends.
3. Colony and sequence Analysis
4. Yeast cell construction
5. Functional anlysis of candidates genes
6. Purification of dihydroniloticin from two strains
7. Gas chromatography - mass spectrometry analysis

Successfully constructed plasmids containing 2,3 oxidosqualene cylase and cytochrome oxidases (Azadirachta indica and Melia azedarach)