LZU-HS-China-C
The Degradation of Sulfadiazine Using Whole-cell Biocatalysts
Challenge
With the development of Animal Husbandry, the requirements of precautions against bacterial infection and the prevention of propagating pathogenic microorganisms have became crucial and necessary. Concerns of human’s health have stimulate austere restrictions in controlling the spread of pathogens in domestic animals. Intensive use of antibiotics have achieve partial success in preventing or eliminating plague on certain extent. Nevertheless, the inevitable results created by overuse of antibiotics have been ignored by humans.
Voluminous amount of antibiotics residue discharged with animal excrement directly leads to the formation of superbugs. Normal antibiotics treatment have less or no effect on patient, which result in severe public medical crisis.
Countermeasures
Preexisting physical and chemical methods have been overthrow since the existence factors of extremely high cost and unobtrusive decomposing efficiency, which also become the main obstacles when designing brand-new biological catalyst. Intensive research and experiment have narrow the range to Laccase, a high-efficient enzyme performed as effective biological catalyst. Among all descriptions of Laccase, Lac6 abstracted from Pleurotus ostreatus could be utilized in degrading antibiotics residue.
Design and Construction
Surface-display technology has been applied in order to achieve extracellular expression of Lac6, utilizing Ecoli Nissle 1917, a non-pathogenic bacterium as the chassis for further constructions of engineering strains, which possessed with various advantages including high efficiency, extended survival period and degradation degree.
Application
Ingestion of engineering strains by domestic animals would lead to severe environmental influences and biological concerns. Instead, lyophilized power of engineering strains could be utilized in degrading animal excretions. In combination with septic tank, advanced hardware designed by our team, antibiotics residue could be degraded effectively for the most part.
Social significance
Destroying the homeostasis and natural development of environment results in extreme negative influence to human society. Public health has been considered as one of the most significant and essential concerns since the tight connections between people’s physical health and development of society. Eliminating potential public health crisis and preventing the existence of superbacteria from all aspects have been our primary goal all along. Furthermore, reinforce the connections between society and biological science will also stimulates concerns of environmental issues and awareness of technological methods.