Improved Parts for 2022

This year, we improved upon the design of our Lbu crRNA from our 2021 project; this year’s crRNA was designed with the help of RNAfold, which allowed us to locate a target sequence that met the ideal design criteria of being close to the 5' end of the sequence, being a relatively unfolded region in the mRNA secondary structure, and having a high probability of remaining single-stranded.