Notebook

7/13

Practicing experimental techniques

8/4

Team meeting

8/5

Collected data on the enzymes used for the experiment

8/8

Collected data on the enzymes used for the experiment

8/9

Collected data on the enzymes used for the experiment

Formed parts used in the experiment via snapgene.

8/10

Formed parts used in the experiment via snapgene.

8/11

Study session on the theme with the members

8/12

Sorted out practical difficulties with the help of the advisors.

8/19

Preparation of all solution that will be used during the experiment.

8/22

Brainstormed ideas for promotion video.

8/23

Protocol printouts and preparation of instruments.

8/24

Wrote down script for promotion video.

8/25

Re-writing the script for the promotion video.

8/29

Making slides for video

8/30

Making slides for video

8/31

Plasmid extraction

9/1

Electrophoresis.

Nanodrop.

Preparation to upload promotion video

9/2

Fixing technical errors of promotion video

9/5

A lesson on Fusion

A lesson on Snapgene

9/6

Plasmid extraction

Electrophoresis

Fusion

nanodrop

9/8

Restriction enzyme treatment

9/9

Electrophoresis

9/14

Calculation of bp via ImageJ

9/19

Gel extraction

Electrophoresis

9/30

SLiCE

Transformation of HMG-CoA reductase front

10/3

Restriction enzyme treatment of synthase and pTAC via Ecor1 and pst1

Colony PCR

Making of master plate

10/4

Restriction enzyme treatment of synthase and pTAC via Ecor1 and pst1

Plasmid extraction of Reductase front in pMD20 in E. coli

10/6

Electrophoresis

PCR and iPCR

Gel extraction

PCR product purification

10/7

Disposal of waste

Electrophoresis

ligation

10/10

PCR and iPCR

10/11

Electrophoresis of PCR and iPCR

Transformation (synthase in pTAC)

Wiki creation.