RBS measurement
This year, we tested and measured an RBS part. We constructed the sequences of T7-RBS-GFP, replacing the default rbs sequence on pETDuet vector into this part BBa_J61117. We first constructed and transformed the sequence into E. coli BL21(DE3) and induced the expression with IPTG overnight. SDS-page results prove the RBS can lead to successful expression of GFP protein. Green color and green florescence can be observed from the bacteria culture.
T7 series promoter
This year, we also detected the promoters of T7 series. We constructed the sequences of C4-GFP, T7-GFP and H9-GFP on pSB1C3 expression vector, and used different promoters to induce the expression of GFP gene. The effect of the promoter was detected by fluorescence intensity. We first constructed and transformed the sequence into E. coli BL21(DE3) and induced the expression with IPTG for 18 h. After that, we could see that the bacterial solution presented different colors, and the bacterial precipitate also presented different colors after centrifugation. According to our results, the effect of T7 promoter is similar to that of H9 promoter, but significantly better than that of C4 promoter, which is consistent with the experimental results from the previous team.
Biology Art Exhibition
In order to promote the popularization of biology, in order to let more people pay attention to the beautiful phenomenon in biology, iBowu_China established a biology art exhibition this year which was held on October 6 to 10, and everyone interested in biology and art was welcomed. We divided the exhibition into two parts, one is biology and aesthetics, and the other is related to AD. We got great feedback from people visited our exhibition, we are looking forward to our biology art exhibition going to other cities, other countries, to show more people the charm of biology.
