We have proof of concept demonstration in measurement, mathematic modeling, home-built photoacoustic device, software, computer programs and engineering bacteria.

A chromatographic technique was established for the separation and quantification of glucose and lactate from complex culture medium (Figure 1). Please visit our Measurement and Results page for more information.

Figure 1. Chromatograms of glucose (converted to 5-hydroxymethylfurfural) and lactate.

A mass spectrometric imaging technique was established for the visualization of changes in metabolome, proteome and enzyme activities (for example, Figure 2). Changes in proteome, metabolome, the network evolvement and enzyme activities in normal and cancerous tissues are visualized with mass spectrometric imaging. With mass spectrometric imaging, biomarkers in the metastatic spread of precancerous lesions can be visualized. Please visit our Measurement and Results page for more information.

Figure 2. Mass spectrometric images that can clearly reveal the differences in normal and cancerous tissues.

Mathematic models were established for epidemiologic survey and monitoring of dynamic metabolism of glucose and lactate. Our established mathematic model has been demonstrated in epidemiological survey of breast cancer and dynamic metabolism of glucose and lactate. A detailed report can be found in our Models page.

A home-built photoacoustic device, software and computer programs have been established for the fundamental testing. In this year, the theoretical principle has been investigated. A home-built device was set up for the fundamental testing. One software with detailed user manual and animation show, two computer programs for fast Fourier transformation and oscilloscopic wave detection and data storage are provided on the our iGEM wiki for free download. Environmental noises can be detected with current device. About three years later, the portable and software may be completed and made as a product available to those people who need it. For detailed information, please visit our Hardware and Software pages. We hope our project are helpful to other people.

The construction of recombinant plasmid containing 3 designed partswas conducted. We have completed the extraction of full-length RNA from breast cancer tumor tissues, reverse transcriptase-polymerase chain reaction (RT-PCR), generation of cDNA for gene cloning, construction of recombinant plasmid and transformation in E. coli. Nissle 1917. Their full functions will be characterized in 2023. The implementation of engineered bacteria may take very long time more than 10 years. You can visit our Project page to see more details.