Parts
All of the sequences encoding proteins presented by our team on the iGEM registry are optimized for an expression in E.coli. Due to high GC content in biobricks optimized for an expression in Streptomyces, we could not put their sequences on the registry (forbidden assembly sites). However, we have also cloned them in Streptomyces specific vectors, in addition to the ones optimized for E.coli.
| Accession number | Biobrick name | Purpose | Link | Status |
|---|---|---|---|---|
| RuBisCO large subunit of S. bottropensis under the control of an RBS | Introduction of the Calvin Cycle | Link | Cloned for an expression in E. coli or in Streptomyces - preliminary data in the native Streptomyces context | |
| RuBisCO small subunit of S. bottropensis under the control of an RBS | Introduction of the Calvin Cycle | Link | Cloned for an expression in E. coli or in Streptomyces | |
| Phosphoribulokinase (PRK) of S. bottropensis under the control of an RBS | Introduction of the Calvin Cycle | Link | Cloned for an expression in E. coli or in Streptomyces - characterized in the native E. coli chassis | |
| KasOP promoter + strong Streptomyces RBS + E. coli promoter + RBS + mRFP | Cloning module, an intermediate construct, mrfp later to be replaced by a Streptomyces gene of interest | Link | Cloned and characterized - shuttle plasmid E. coli / Streptomyces | |
| single guide RNA targeting one of the lsr2 paralogs | Silencing the specialized metabolite biosynthetic gene clusters repressor => increasing antibiotic production | Link | Cloned and introduced by conjugation into Streptomyces | |
| single guide RNA targeting the gpm gene | silencing the glycerophosphate mutase gene to force the Calvin cycle | Link | Cloned, introduced by conjugation and characterized into Streptomyces ambofaciens ATCC 23877 |